{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:27:57Z","timestamp":1787776077881,"version":"build-2784847793"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,10]]},"abstract":"\n Background & objectives:<\/jats:title>\n \n Cutaneous leishmaniasis (CL) is a vector-borne parasitic disease caused by several species of the protozoan parasite\n Leishmania.<\/jats:italic>\n The need for new anti-leishmanial drugs for the treatment of CL is complicated by factors such as high cost, toxicity, potential for resistance and limited long-term use of existing anti-leishmanial drugs. The aim of this study was to investigate the therapeutic effect of\n Tarantula cubensis<\/jats:italic>\n alcoholic extract (TCE), which has been shown to have wound-healing, anti-inflammatory, regenerative, resolving and epithelialising effects, on\n Leishmania major<\/jats:italic>\n promastigotes\n in vitro<\/jats:italic>\n and\n in vivo<\/jats:italic>\n in an experimental mouse model of CL.\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n The effect of TCE on\n L. major<\/jats:italic>\n promastigotes\n in vitro<\/jats:italic>\n was investigated after determining non-cytotoxic concentrations of TCE using the XTT method. To establish a CL model,\n L. major<\/jats:italic>\n amastigotes were injected into the paws of BALB\/c mice. Lesion size and histopathological evaluation were used to assess the effect of treatment.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n TCE was found to be effective against\n L. major<\/jats:italic>\n promastigotes at 24 h and 48 h at concentrations 250 \u03bcg\/ mL, 125 \u03bcg\/mL and 62.5 \u03bcg\/mL (\n P<\/jats:italic>\n <0.05). TCE was found to be more effective than meglumine antimonate in treating CL in the experimentally induced CL model in BALB\/c mice.\n <\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusion:<\/jats:title>\n The results suggest that TCE holds promising potential as a therapeutic agent for the treatment of CL. However, further extensive investigations are required to substantiate and expand our understanding in this area.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jvbd.jvbd_55_24","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T11:01:08Z","timestamp":1717671668000},"page":"594-600","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Investigation of in vitro and in vivo therapeutic activity of Tarantula cubensis extract (Theranekron\u00ae) on Leishmania major"],"prefix":"10.4103","volume":"61","author":[{"given":"Elif","family":"Akyol","sequence":"first","affiliation":[{"name":"Departments of Medical Parasitology, Cumhuriyet University School of Medicine, Sivas, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z\u00fcbeyda Ak\u0131n","family":"Polat","sequence":"additional","affiliation":[{"name":"Departments of Medical Parasitology, Cumhuriyet University School 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