{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:38:50Z","timestamp":1786649930275,"version":"build-2736575974"},"reference-count":17,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,1]]},"abstract":"\n Background:<\/jats:title>\n \n The presence of\n BCR<\/jats:italic>\n -\n ABL1<\/jats:italic>\n fusion gene resulting from a t(9; 22) reciprocal chromosome translocation is the molecular hallmark of chronic myeloid leukemia (CML). In the diagnosis and treatment of CML, peripheral blood or bone marrow samples are usually taken for analysis. However, both methods are invasive sample collection methods, thus a noninvasive saliva sample method for the detection of the fusion gene transcripts (\n BCR<\/jats:italic>\n -\n ABL<\/jats:italic>\n ) was investigated in some Nigerians with CML.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n Real-time (RT)-polymerase chain reaction (PCR) analysis was used to detect\n BCR<\/jats:italic>\n -\n ABL1<\/jats:italic>\n fusion gene in the saliva and blood of 42 Nigerian CML patients. RNA was extracted using RNeasy kit and reverse transcribed by random hexamer priming using murine Moloney reverse transcriptase.\n BCR<\/jats:italic>\n -\n ABL1<\/jats:italic>\n transcript types were first detected by multiplex PCR and then quantified by a duplex RT-PCR-TaqMan chemistry with MGB probe and Black Hole Quencher.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Of the 42 subjects, transcript types were detected in 36 (85.7%) samples, e13a2 fusion transcript sub-type was detected in 9 (21.4%), whereas e14a2 subtype was found in 27 (67.3%); six (14.3%) of the samples did not reveal any of the fusion transcript subtypes. The median\n BCR<\/jats:italic>\n -\n ABL1<\/jats:italic>\n messenger RNA values were 9.38 \u00d7 10\n 2<\/jats:sup>\n in saliva and 10.29 \u00d7 10\n 4<\/jats:sup>\n in blood (\n P<\/jats:italic>\n < 0.05). Similarly, the median\n ABL1<\/jats:italic>\n value in saliva (3.11 \u00d7 10\n 3<\/jats:sup>\n ) was significantly lower (\n P<\/jats:italic>\n < 0.01) than in blood (4.22 \u00d7 10\n 3<\/jats:sup>\n ). However, the median\n BCR<\/jats:italic>\n -\n ABL1<\/jats:italic>\n ratio in saliva (14.5%) was not significantly different (\n P<\/jats:italic>\n = 0.8) from that of blood (12.0%).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n Saliva may offer an alternative easy-to-collect, readily available, and noninvasive sample for the diagnosis and treatment of CML.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/njcp.njcp_225_18","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T06:21:14Z","timestamp":1692598874000},"page":"51-55","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":3,"title":["Detection of BCR-ABL1 Fusion Gene Transcripts in the Saliva of Nigerian Patients with Chronic Myeloid Leukemia"],"prefix":"10.4103","volume":"22","author":[{"given":"IC","family":"Uzoma","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Enugu, Nigeria"},{"name":"Department of Cell Biology and Genetics, Genetics Laboratory, Faculty of Science, University of Lagos, Lagos, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"IA","family":"Taiwo","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics, Genetics Laboratory, Faculty of Science, University of Lagos, Lagos, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"EO","family":"Nna","sequence":"additional","affiliation":[{"name":"The Molecular Pathology Institute, Safety Molecular Pathology Laboratory, Enugu, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MA","family":"Durosinmi","sequence":"additional","affiliation":[{"name":"Department of Haematology, Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"EO","family":"Ukaejiofo","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Enugu, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-20251006","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1182\/blood-2008-03-144790","article-title":"Molecular biology of bcr-abl1-positive chronic myeloid leukemia","volume":"113","author":"Quintas-Cardama","year":"2009","journal-title":"Blood"},{"key":"R2-20251006","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/978-1-61779-074-4_4","article-title":"Chronic myeloid leukemia: Cytogenetic methods and applications for diagnosis and treatment","volume":"730","author":"Morris","year":"2011","journal-title":"Methods Mol Biol"},{"key":"R3-20251006","first-page":"430","article-title":"New insights into the 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