{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:05:41Z","timestamp":1787169941644,"version":"build-2736575974"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Malaria rapid diagnostic tests (mRDTs) are the preferred option for programmatic deployment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>There are numerous mRDTs on the Nigerian market and there is a need to guide practitioners on the relative performance of the commonly used brands of mRDT in Nigeria.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Subjects and Methods:<\/jats:title>\n                    <jats:p>The performance of three commonly used Histidine-Rich-Protein-2-based mRDTs (SD-Bioline\u2122, Carestart\u2122 and Paracheck-Pf\u2122) against microscopy of Giemsa stained blood and polymerase chain reaction (PCR) was evaluated among 190 febrile under-5 children in Ibadan, Nigeria. We calculated the sensitivity, specificity, predictive values, accuracy, and agreements.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      There were 53.2% males. The prevalence of malaria parasite by microscopy was 46.8% and 57.9% by PCR. Malaria parasite detection by SD-Bioline\u2122 was 60.5%, Carestart\u2122: 60.0% and Paracheck-\n                      <jats:italic toggle=\"yes\">Pf<\/jats:italic>\n                      \u2122 60.0%. Using microscopy as the gold standard, the sensitivities of SD-Bioline\u2122, Carestart\u2122 and Paracheck-\n                      <jats:italic toggle=\"yes\">Pf<\/jats:italic>\n                      \u2122 mRDT were 97.8%, 96.7% and 97.8% respectively while the specificities were 73.0%, 72.0% and 74.0% respectively. Using PCR as the gold standard, the sensitivity for both SD-Bioline\u2122 and Paracheck-\n                      <jats:italic toggle=\"yes\">Pf<\/jats:italic>\n                      \u2122 was 85.5% and for CareStart was 84.6% while the specificity of SD-Bioline\u2122, Carestart\u2122, and Paracheck-\n                      <jats:italic toggle=\"yes\">Pf<\/jats:italic>\n                      \u2122 was 73.8%, 72.4%, and 75.0% respectively. The test accuracy was 81.0% for both SD-Bioline\u2122 and Paracheck-\n                      <jats:italic toggle=\"yes\">Pf<\/jats:italic>\n                      \u2122 and 80.0% for Caresatrt\u2122. The kappa coefficient of agreement between PCR and each of SD-Bioline\u2122, Carestart, ParaCheck\u2122 and microscopy was 0.597, 0.578, 0.609 and 0.739 respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The performance of the three mRDTs is a proof that any of the three is suitable for use in the diagnosis of malaria in the southwest of Nigeria.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/njcp.njcp_491_20","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T03:28:40Z","timestamp":1692588520000},"page":"496-504","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":8,"title":["Comparative Evaluation of Three Histidine-Rich Protein-2 Based Rapid Diagnostic Tests, Microscopy and PCR for Guiding Malaria Treatment in Ibadan, Southwest 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