{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:36:06Z","timestamp":1786649766820,"version":"build-2736575974"},"reference-count":38,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>The aim of the present study was to evaluate bone maturation in a standardized bone defect model after the application of a treated tooth graft (TTG) formed from demineralized dentin loaded with statin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>In this study, 30 adult male rabbits (weight 2 kg to 2.5 kg) were used, and they were divided equally into two groups: control and TTG groups. In each rabbit, two standardized round bone defects were created (one in each tibia), where the defects were left without grafting in the control group and were packed with TTG slices in the study group. TTG is formed of demineralized dentin matrix slices loaded with statin. The fifteen rabbits in each group were separated into three subgroups based on postoperative evaluation intervals (2, 4, and 6 weeks). The animals were euthanized at 2, 4 and 6 weeks postoperatively, and half of the bone specimens were processed for histological examination using Masson\u2019s trichrome special stain, while the other half was prepared for analysis using energy-dispersive X-ray spectroscopy (EDX).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The histomorphometry analysis results showed a significantly greater mean of red-staining bone matrix area for the TTG group, suggesting that the new bone matrix was more mature. Enhanced collagen maturation appeared histologically coupled with an increase in mineral contents, as indicated by SEM-EDX results, where the concentrations of Ca and P increased significantly with time in TTG group (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2264 0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>TTG group consistently showed higher levels of Ca and P and lower C\/(Ca+P) ratios compared to the Control group. TTG was able to fasten the maturation of bone formation during bone defect healing.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/njms.njms_169_24","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T09:00:18Z","timestamp":1766739618000},"page":"440-446","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of bone maturation following application of treated tooth bio-graft in bone defects: Experimental animal study"],"prefix":"10.4103","volume":"16","author":[{"given":"Mena F.","family":"Abd-Allah","sequence":"first","affiliation":[{"name":"Department of Oral Biology, Al-Azhar University, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abeer S.","family":"Gawish","sequence":"additional","affiliation":[{"name":"Dean of Graduate Studies Sinai University ELARISH and Department of Oral Medicine, Periodontology Oral Diagnosis and Radiology, Al-Azhar University, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abd el Rahman O.","family":"El Mekkawi","sequence":"additional","affiliation":[{"name":"Department of Endodontics, Faculty of Dentistry, Sini University, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nouralhouda F.","family":"Abd-Allah","sequence":"additional","affiliation":[{"name":"Department of Pedodontics and Oral Health, Al-Azhar University, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia A.","family":"Radi","sequence":"additional","affiliation":[{"name":"Department of Oral and Dental Pathology, Al-Azhar University, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma Mahmoud","family":"Abdel-Rahman","sequence":"additional","affiliation":[{"name":"Department of Dental Biomaterials Department, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah","family":"Saeidi","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diagnostic Sciences, Taibah University, College of Dentistry, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shadia A.","family":"Elsayed","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Al-Azhar University, Cairo, Egypt"},{"name":"Department of Oral and Maxillofacial Diagnostic Sciences, Taibah University, College of Dentistry, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inas M.","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Dental Biomaterials, Dental Biomaterials Department, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,12,25]]},"reference":[{"key":"R1-20260420","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1097\/00003086-198604000-00036","article-title":"The critical size defect as an experimental model for craniomandibulofacial nonunions","author":"Schmitz","year":"1986","journal-title":"Clin Orthop Relat Res"},{"key":"R2-20260420","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1186\/s13287-021-02642-w","article-title":"Bone regeneration in rat calvarial defects using dissociated or spheroid mesenchymal stromal cells in scaffold-hydrogel constructs","volume":"12","author":"Shanbhag","year":"2021","journal-title":"Stem Cell Res Ther"},{"key":"R3-20260420","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1111\/jcpe.13816","article-title":"Clinical efficacy of soft-tissue augmentation on tissue preservation at immediate implant sites: A randomized controlled trial","volume":"50","author":"Lee","year":"2023","journal-title":"J Clin Periodontol"},{"key":"R4-20260420","first-page":"66","article-title":"the sinus lift procedure applied in cases where the thickness of the alveolar bone is insufficient using double Prf as well as in the case of an intrasinus mucocele","volume":"15","author":"Mitrea","year":"2023","journal-title":"Rom J Oral Rehabil"},{"key":"R5-20260420","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.joms.2022.10.008","article-title":"Comparative effects of different materials on alveolar preservation","volume":"81","author":"Rodrigues","year":"2023","journal-title":"J Oral Maxillofac Surg Off J Am Assoc Oral Maxillofac Surg"},{"key":"R6-20260420","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1016\/j.joen.2008.05.004","article-title":"Metgud S. 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