{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:38:49Z","timestamp":1786649929384,"version":"build-2736575974"},"reference-count":14,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>A key player in the development of colorectal carcinoma is p63, a protein belonging to the p53 family. Tumorigenesis, invasion and metastasis are linked to its elevated expression in certain malignancies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim:<\/jats:title>\n                    <jats:p>In this study, we aimed to investigate the immunohistochemical expression of p63 in colorectal carcinoma along with its correlation to clinicopathological parameters and its precursor lesion colorectal adenoma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>This study used 49 formalin-fixed paraffin-embedded tissue sections: 16 surgically resected (14 carcinomas and 2 adenomas) and 33 colonoscopy biopsies (28 carcinomas and 5 adenomas). Tumour characteristics (size and location) and demographic data (age and sex) were obtained from the archive system. Haematoxylin- and eosin-stained sections were reassessed for histological grade, subtype, lymphovascular invasion, invasion depth, lymph nodes and metastasis. Statistical analysis was performed with Fisher\u2019s exact test, Microsoft Excel and SPSS Version 21. H-Score was used for immunohistochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      P63 expression was absent in normal mucosa, while P63 immunohistochemistry was positive in 43 (88%) cases. Forty-two (86%) out of 49 cases showed cytoplasmic expression of p63, of which 35 cases (83.3%) were carcinomas. P63 expression revealed a significant correlation with histological subtype (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001), histological grade (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001), distant metastasis (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.033), tumour, node and metastasis\/American Joint Committee on Cancer (TNM\/AJCC) stage (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.049) and between colorectal carcinoma and adenoma (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Moderate-to-strong cytoplasmic p63 expression was seen only in malignancy, suggesting its role in carcinogenesis. Increased p63 staining intensity from low- to high-grade tumours indicates p63 as a marker of poor differentiation. The correlation between metastasis and stronger p63 expression with higher TNM\/AJCC stages confirms elevated p63 in aggressive tumours.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/npmj.npmj_297_24","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T04:00:35Z","timestamp":1742184035000},"page":"53-60","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["A Study of Immunohistochemical Expression of p63 in Colorectal Carcinoma"],"prefix":"10.4103","volume":"32","author":[{"given":"Aishwarya Prasad","family":"Nair","sequence":"first","affiliation":[{"name":"Department of Pathology, Sree Balaji Medical College and Hospital, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanka","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Pathology, Sree Balaji Medical College and Hospital, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sai Sudha","family":"Muddha","sequence":"additional","affiliation":[{"name":"Department of Pathology, Sree Balaji Medical College and Hospital, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,3,17]]},"reference":[{"key":"R1-20260423","first-page":"394","article-title":"Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"68","author":"Bray","year":"2018","journal-title":"CA Cancer J Clin"},{"key":"R2-20260423","doi-asserted-by":"crossref","first-page":"852927.","DOI":"10.3389\/fonc.2022.852927","article-title":"Molecular network of colorectal cancer and current therapeutic options","volume":"12","author":"Huang","year":"2022","journal-title":"Front Oncol"},{"key":"R3-20260423","doi-asserted-by":"crossref","first-page":"432","DOI":"10.15537\/smj.2019.5.24162","article-title":"The prognostic significance of p63 cytoplasmic expression in colorectal cancer. An immunohistochemical study","volume":"40","author":"Albasri","year":"2019","journal-title":"Saudi Med J"},{"key":"R4-20260423","doi-asserted-by":"crossref","first-page":"e212","DOI":"10.5301\/JBM.2012.9581","article-title":"p63 expression is a prognostic factor in colorectal cancer","volume":"27","author":"Guo","year":"2012","journal-title":"Int J Biol Markers"},{"key":"R5-20260423","doi-asserted-by":"crossref","first-page":"773","DOI":"10.15537\/smj.2018.8.22837","article-title":"Impact of cyclooxygenase-2 over-expression on the prognosis of colorectal cancer patients. An experience from Western Saudi Arabia","volume":"39","author":"Albasri","year":"2018","journal-title":"Saudi Med J"},{"key":"R7-20260423","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0022-5347(05)67975-X","article-title":"Multivariate analysis of the prognostic factors of primary superficial bladder cancer","volume":"163","author":"Mill\u00e1n-Rodr\u00edguez","year":"2000","journal-title":"J Urol"},{"key":"R9-20260423","doi-asserted-by":"crossref","first-page":"2458","DOI":"10.1038\/sj.emboj.7600708","article-title":"TAp63alpha induces apoptosis by activating signaling via death receptors and mitochondria","volume":"24","author":"Gressner","year":"2005","journal-title":"EMBO J"},{"key":"R10-20260423","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.bbrc.2010.04.093","article-title":"Dominant negative (DeltaN) p63alpha induces drug resistance in hepatocellular carcinoma by interference with apoptosis signaling pathways","volume":"396","author":"Mundt","year":"2010","journal-title":"Biochem Biophys Res Commun"},{"key":"R12-20260423","doi-asserted-by":"crossref","first-page":"6206","DOI":"10.3748\/wjg.v21.i20.6206","article-title":"Expression of COX-2 and HER-2 in colorectal cancer and their correlation","volume":"21","author":"Wu","year":"2015","journal-title":"World J Gastroenterol"},{"key":"R13-20260423","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.prp.2005.04.006","article-title":"Prognostic importance of COX-2 expression in patients with colorectal cancer","volume":"201","author":"Yamac","year":"2005","journal-title":"Pathol Res Pract"},{"key":"R14-20260423","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1038\/cdd.2015.53","article-title":"TP53: An oncogene in disguise","volume":"22","author":"Soussi","year":"2015","journal-title":"Cell Death Differ"},{"key":"R15-20260423","first-page":"M31","article-title":"Diverse nuclear transport pathways regulate cell proliferation and oncogenesis","volume":"1471","author":"Hood","year":"2000","journal-title":"Biochim Biophys Acta"},{"key":"R16-20260423","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1111\/j.1365-2559.2006.02507.x","article-title":"Cytoplasmic localization of p63 is associated with poor patient survival in lung adenocarcinoma","volume":"49","author":"Narahashi","year":"2006","journal-title":"Histopathology"},{"key":"R17-20260423","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1158\/1055-9965.EPI-08-0785","article-title":"Aberrant cytoplasmic expression of p63 and prostate cancer mortality","volume":"18","author":"Dhillon","year":"2009","journal-title":"Cancer Epidemiol Biomarkers Prev"}],"container-title":["Nigerian Postgraduate Medical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/npmj.npmj_297_24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:00:05Z","timestamp":1786647605000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/npmj.npmj_297_24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.4103\/npmj.npmj_297_24","relation":{},"ISSN":["1117-1936","2468-6875"],"issn-type":[{"value":"1117-1936","type":"print"},{"value":"2468-6875","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-12-02","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-05","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}