{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:36:59Z","timestamp":1787071019891,"version":"build-2736575974"},"reference-count":14,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Varicose veins are a common condition affecting millions of people worldwide. While they are often considered primarily a cosmetic issue, varicose veins can also lead to pain, swelling, and discomfort. Histopathological studies are valuable for examining the structure and composition of varicose veins. These studies provide important insights into the underlying causes of the condition and help inform treatment options for patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>To evaluate the distribution of collagen and elastin in the venous walls using Masson's Trichrome and Verhoeff\u2019s Van Gieson staining.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>This study examines histological changes in varicose veins, focusing on the distribution of collagen and elastin through Masson's Trichrome and Verhoeff's Van Gieson staining methods. This study examined histological samples of varicose veins from 30 individuals. The tissue samples were fixed in formalin and stained with Hematoxylin &amp; Eosin, Masson's Trichrome, and Verhoeff's Van Gieson stains, followed by analysis using light microscopy. Statistical analysis was performed with IBM SPSS version 23.0.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Histological evaluation revealed significant changes in the tunica media, including a loss of smooth muscle (86.67%) and increased collagen deposition (76.67%). The intima exhibited prevalent intimal thickening (96.67%) and fibrosis (93.33%), while the adventitia showed notable adventitial fibrosis (86.67%) and vascular remodeling (53.33%). Elastic fiber impairment was observed in 13.33% of individuals, with collagen as the main structural component in all venous layers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Histopathological studies offer valuable insights into the structural changes that occur in varicose veins through the use of special stains. These studies play a crucial role in the diagnosis and management of varicose veins by providing detailed information about tissue structure and pathology. This information helps healthcare providers deliver personalized care to patients affected by this condition. Continued research in this field will further enhance our understanding of varicose veins and improve patient outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/npmj.npmj_42_25","type":"journal-article","created":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T06:00:18Z","timestamp":1754028018000},"page":"187-191","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Histopathological Study of Varicose Veins with Special Emphasis on Masson Trichrome and Verhoeff\u2019s van Gieson Stains"],"prefix":"10.4103","volume":"32","author":[{"given":"P. Roshan","family":"Parveen","sequence":"first","affiliation":[{"name":"Department of Pathology, ACS Medical College and Hospital, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Pavithra","sequence":"additional","affiliation":[{"name":"Department of Pathology, ACS Medical College and Hospital, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Sudha","sequence":"additional","affiliation":[{"name":"Department of Pathology, ACS Medical College and Hospital, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Vimal","family":"Chander","sequence":"additional","affiliation":[{"name":"Department of Pathology, ACS Medical College and Hospital, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Prakashiny","sequence":"additional","affiliation":[{"name":"Department of Pathology, ACS Medical College and Hospital, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,8,1]]},"reference":[{"key":"R1-20260421","doi-asserted-by":"crossref","first-page":"d3865","DOI":"10.1136\/bmj.d3865","article-title":"The association between venous thromboembolism and physical inactivity in everyday life","volume":"343","author":"Douketis","year":"2011","journal-title":"BMJ"},{"key":"R3-20260421","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.3390\/healthcare11243183","article-title":"Prevalence of varicose veins and its risk factors among nurses working at king Khalid 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