{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T12:27:54Z","timestamp":1786969674005,"version":"build-2736575974"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Periodontitis is a chronic multifactorial disease influenced by genetic susceptibility. Identifying genetic markers may facilitate early detection and improved risk assessment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim:<\/jats:title>\n                    <jats:p>To evaluate the association between tumour necrosis factor-alpha (TNF-\u03b1) gene polymorphisms and periodontitis in a North Indian population.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Subjects and Methods:<\/jats:title>\n                    <jats:p>\n                      A total of 364 participants were recruited and divided into two groups: Group A (healthy;\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 182) and Group B (periodontitis;\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 182). Detailed case histories were recorded, and buccal swab samples were collected and stored at 4\u00b0C in a sterile medium. Genomic DNA was extracted and analysed using tetraprimer amplification refractory mutation system polymerase chain reaction and Sanger sequencing. Allele frequencies were calculated using Hardy\u2013Weinberg equilibrium and Chi-square tests (GenAlEx software). Linkage disequilibrium and haplotype analyses were performed using SHEsis. Correlation analysis was conducted to assess the association between TNF-\u03b1 polymorphisms and disease prevalence.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>A high frequency of TNF-\u03b1 polymorphisms was observed in the North Indian population. Linkage analysis revealed that single-nucleotide polymorphisms (SNPs) loci \u2212278A\/C and \u2212378C\/T were significantly associated with both healthy and periodontitis groups compared to other SNPs. The \u2212308G\/A polymorphism was not detected in the study population.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>TNF-\u03b1 gene polymorphisms are prevalent in the North Indian population. SNP loci \u2212278A\/C and \u2212378C\/T demonstrate significant association with periodontal status and may serve as potential genetic markers for susceptibility to periodontitis. Further studies are warranted to validate these findings in larger and diverse populations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/npmj.npmj_567_25","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T04:00:25Z","timestamp":1786939225000},"page":"630-636","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Association of Tumour Necrosis Factor-Alpha with Periodontitis Patients in North Indian Population"],"prefix":"10.4103","volume":"33","author":[{"given":"Ruchi","family":"Pandey","sequence":"first","affiliation":[{"name":"Department of Periodontology, Manav Rachna Dental College, SDS, MRIIRS, Faridabad, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumit","family":"Bhateja","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Radiology, Manav Rachna Dental College, SDS, MRIIRS, Faridabad, Haryana, 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