{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:22:24Z","timestamp":1787775744223,"version":"build-2784847793"},"reference-count":60,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T00:00:00Z","timestamp":1720569600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:p>JOURNAL\/nrgr\/04.03\/01300535-202602000-00040\/figure1\/v\/2026-04-22T115157Z\/r\/image-tiff<\/jats:p>\n                  <jats:p>\n                    AAV-PHP.eB is an artificial adeno-associated virus (AAV) that crosses the blood\u2013brain barrier and targets neurons more efficiently than other AAVs when administered systematically. While AAV-PHP.eB has been used in various disease models, its cellular tropism in cerebrovascular diseases remains unclear. In the present study, we aimed to elucidate the tropism of AAV-PHP.eB for different cell types in the brain in a mouse model of ischemic stroke and evaluate its effectiveness in mediating basic fibroblast growth factor (\n                    <jats:italic toggle=\"yes\">bFGF<\/jats:italic>\n                    ) gene therapy. Mice were injected intravenously with AAV-PHP.eB either 14 days prior to (pre-stroke) or 1 day following (post-stroke) transient middle cerebral artery occlusion. Notably, we observed a shift in tropism from neurons to endothelial cells with post-stroke administration of AAV-PHP.eB-mNeonGreen (mNG). This endothelial cell tropism correlated strongly with expression of the endothelial membrane receptor lymphocyte antigen 6 family member A (Ly6A). Furthermore, AAV-PHP.eB-mediated overexpression of bFGF markedly improved neurobehavioral outcomes and promoted long-term neurogenesis and angiogenesis post\u2013ischemic stroke. Our findings underscore the significance of considering potential tropism shifts when utilizing AAV-PHP.eB-mediated gene therapy in neurological diseases and suggest a promising new strategy for\n                    <jats:italic toggle=\"yes\">bFGF<\/jats:italic>\n                    gene therapy in stroke treatment.\n                  <\/jats:p>","DOI":"10.4103\/nrr.nrr-d-23-01802","type":"journal-article","created":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T04:12:30Z","timestamp":1720757550000},"page":"704-714","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":5,"title":["Tropism-shifted AAV-PHP.eB-mediated bFGF gene therapy promotes varied neurorestoration after ischemic stroke in mice"],"prefix":"10.4103","volume":"21","author":[{"given":"Rubing","family":"Shi","sequence":"first","affiliation":[{"name":"Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 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