{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:29:30Z","timestamp":1786649370441,"version":"build-2736575974"},"reference-count":36,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background and purpose:<\/jats:title>\n                    <jats:p>\n                      To evaluate the effects of\n                      <jats:italic toggle=\"yes\">Vitex agnus-castus<\/jats:italic>\n                      supplementation on serum levels of inflammatory cytokines (primary outcomes: tumor necrosis factor-alpha (TNF-\u03b1), interleukin-6 (IL-6); secondary outcome: anti-Mullerian hormone (AMH)) in women with polycystic ovary syndrome (PCOS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental approach:<\/jats:title>\n                    <jats:p>\n                      This secondary analysis included 40 serum samples (20\n                      <jats:italic toggle=\"yes\">Vitex<\/jats:italic>\n                      and 20 placebo) randomly selected from a previously registered randomized, double-blind, placebo-controlled trial in women with PCOS. Participants received either\n                      <jats:italic toggle=\"yes\">Vitex<\/jats:italic>\n                      (5.8 mg\/day, standardized to 0.42-0.82 mg aucubin) or a placebo for 12 weeks. Serum TNF-\u03b1, IL-6, and AMH were measured at baseline and week 12 using ELISA. Within- group changes were assessed with paired t-tests, and between-group differences were analyzed using independent t-tests and ANCOVA using SPSS v23.0 software.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Findings\/Results:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Vitex<\/jats:italic>\n                      supplementation significantly reduced serum TNF-\u03b1 (141.27 \u00b1 24.27 to 91.28 \u00b1 19.57 pg\/mL), IL-6 (71.72 \u00b1 13.63 to 48.91 \u00b1 24.19 pg\/mL), and AMH (11.21 \u00b1 3.89 to 7.88 \u00b1 4.87 ng\/mL) levels, whereas the placebo group showed no significant changes. The between-group differences were significant for all markers. These findings suggest potential benefits in reducing inflammation and improving ovarian function, pending confirmation in future clinical outcome studies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and implications:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Vitex<\/jats:italic>\n                      supplementation may offer a complementary approach to managing inflammation and hormonal disturbances in women with PCOS.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/rps.rps_152_25","type":"journal-article","created":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T04:00:08Z","timestamp":1777176008000},"page":"206-214","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["The effects of Vitex agnus-castus supplementation on inflammatory markers in women with PCOS: a randomized, double-blind, placebocontrolled trial"],"prefix":"10.4103","volume":"21","author":[{"given":"Aniseh","family":"Hatami","sequence":"first","affiliation":[{"name":"Student Research Committee, Arak University of Medical Sciences, Arak, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanieh","family":"Taheri","sequence":"additional","affiliation":[{"name":"Student Research Committee, Arak University of Medical Sciences, Arak, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Khosrowbeygi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Genetics, School of Medicine, Arak University of Medical Sciences, Arak, Iran"},{"name":"Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fateme","family":"Seidi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, School of Medicine, Arak University of Medical Sciences, Arak, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohadeseh","family":"Azimi","sequence":"additional","affiliation":[{"name":"Student Research Committee, Arak University of Medical Sciences, Arak, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farideh","family":"Jalali-Mashayekhi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Genetics, School of Medicine, Arak University of Medical Sciences, Arak, Iran"},{"name":"Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, 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