{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:01:26Z","timestamp":1787169686472,"version":"build-2736575974"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,5]]},"abstract":"\n Background:<\/jats:title>\n Intestinal inflammation caused by sleep restriction (SR) threatens human health. However, radical cure of intestinal inflammatory conditions is considerably difficult. This study focuses on the effect of melatonin on SR-induced intestinal inflammation and microbiota imbalance in mice.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n We successfully established a water platform to induce long-term SR in mice for 28 days with or without melatonin supplementation. The SR-induced oxidative stress and inflammatory changes were evaluated in plasma and jejunum tissue samples using\n in vitro<\/jats:italic>\n assays. Additionally, changes in the intestinal microbiota were explored using high-throughput sequencing of the 16S rRNA gene.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n After 20 h of chronic sleep restriction for 28 consecutive days, plasma melatonin was significantly reduced by 48.91% (\n P<\/jats:italic>\n < 0.05), while GLU, NE, and CORT were significantly increased (34.32%\u201390.28%,\n P<\/jats:italic>\n < 0.05). The activities of antioxidant enzymes (SOD, GSH-Px, and CAT) and T-AOC in intestinal tissues of SR mice were decreased (17.02%\u201340.92%,\n P<\/jats:italic>\n < 0.05), while the content of MDA was increased (15.12%,\n P<\/jats:italic>\n = 0.0089). The levels of pro-inflammatory cytokines (IL-6 and TNF-\u03b1) ware increased (65.27%\u2013123.26%,\n P<\/jats:italic>\n < 0.05), while the levels of anti-inflammatory cytokines (IL-10 and IFN-\u03b3) were decreased (26.53%\u201360.41%,\n P<\/jats:italic>\n < 0.05). High-throughput pyrosequencing of 16S rRNA from jejunum samples demonstrated an overall increase in the number of OTUs (30.68%,\n P<\/jats:italic>\n = 0.015). The \u03b1-diversity (Shannon, ACE and Chao1) of jejunum was increased (28.18%\u201348.95%,\n P<\/jats:italic>\n < 0.05), and the \u03b2-diversity (PCoA and NMDS) was significantly different from that of the control group (\n P<\/jats:italic>\n = 0.001). Furthermore, the prevalences of Helicobacter and Clostridium were higher, whereas that of Bacteroidetes and Lactobacillus were lower in SR mice than in controls (\n P<\/jats:italic>\n < 0.05). However, melatonin supplementation reversed the SR-induced changes and improved oxidative stress, inflammatory response, and microbiota dysbiosis in the jejunum, and there was not significant difference compared with the control group (\n P<\/jats:italic>\n > 0.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n Melatonin prevents the dysbiosis of intestinal microbiota in SR mice by improving oxidative stress and inhibiting inflammation. Our results may provide a theoretical basis for conducting clinical research on insufficient sleep leading to intestinal health in humans and hence facilitate a better understanding of the role of melatonin.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/sjg.sjg_110_21","type":"journal-article","created":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T05:12:47Z","timestamp":1646284367000},"page":"209-217","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":28,"title":["Melatonin prevents the dysbiosis of intestinal microbiota in sleep-restricted mice by improving oxidative stress and inhibiting 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