{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:28:53Z","timestamp":1786649333403,"version":"build-2736575974"},"reference-count":11,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Context:<\/jats:title>\n                    <jats:p>Laboratory diagnosis of strongyloidiasis is most often based on the detection of antibodies against antigens in an enzyme-linked immunosorbent assay (ELISA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>\n                      The aim of the study was to validate the efficacy of the B-cell epitope peptide antigens of fatty acid and retinal binding protein in using antibodies against\n                      <jats:italic toggle=\"yes\">Strongyloides stercoralis<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Settings and Design:<\/jats:title>\n                    <jats:p>Patient samples were identified by an anonymous code and collected from volunteers at Suranaree University of Technology Hospital, Thailand.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Subjects and Methods:<\/jats:title>\n                    <jats:p>\n                      The synthesized peptides and cell lysate of\n                      <jats:italic toggle=\"yes\">Strongyloides ratti<\/jats:italic>\n                      were used as antigens in an in-house ELISA format. A total of 78 serum samples were collected from patients infected with\n                      <jats:italic toggle=\"yes\">S. stercoralis<\/jats:italic>\n                      , other parasites, and healthy persons.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Statistical Analysis Used:<\/jats:title>\n                    <jats:p>The receiver operating characteristic curve was used to assess the diagnostic performance of peptide antigens and analysis of variance was utilized to examine differences in peptide reactivity between groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The peptide-1 and cell lysate of\n                      <jats:italic toggle=\"yes\">S. ratti<\/jats:italic>\n                      could clearly differentiate serums among infected and noninfected groups. The peptide-1, peptide-2 of\n                      <jats:italic toggle=\"yes\">S. stercoralis,<\/jats:italic>\n                      and lysate of\n                      <jats:italic toggle=\"yes\">S. ratti<\/jats:italic>\n                      antigen had been measured to reveal sensitivities at about 75.00%, 50.00%, and 75.00% with 75.86%, 86.21%, and 89.66% specificities, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>These results could be useful for routine clinical laboratory diagnostic testing and possibly apply for surveillance investigations in the future.<\/jats:p>\n                  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