{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T03:20:51Z","timestamp":1761621651317,"version":"3.41.0"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:sec>\n            <jats:title>Background:<\/jats:title>\n            <jats:p>Conventional acrylic resin is prone to microbial colonization and may cause inflammatory and allergic response.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Aims:<\/jats:title>\n            <jats:p>This study aims to research the initial microbial adhesion <jats:italic toggle=\"yes\">in situ<\/jats:italic> and tissue response to an acrylic resin used in prosthodontics.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Materials and Methods:<\/jats:title>\n            <jats:p>Disks of a commercial acrylic resin were prepared and included on the surface of individual intraoral splints fabricated for 50 participants. The splints were used for 4 h, under clinical conditions. Beforehand, each participant was swabbed to provide a control for microbiological comparison. A cytological control sample was also taken from the palate. After the time elapsed, each splint was removed and growth of anaerobes, aerobes, <jats:italic toggle=\"yes\">Pseudomonas<\/jats:italic>, oral streptococci, staphylococci, yeasts, and <jats:italic toggle=\"yes\">Streptococcus mutans<\/jats:italic> was determined by plate counts and compared to the oral microbiome. A cytological sample was taken from the contact zone, stained using the Papanicolaou technique, analyzed in light microscopy, and classified accordingly. Means and standard deviations were calculated, and a nonparametric Wilcoxon test was employed to compare experimental groups. The significance level was set at 0.05 (95% confidence interval, and statistical analysis was performed using Statistical Package for the Social Sciences (SPSS) version 20.0.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>Nuclear-cytoplasm ratio increase was found in 84% of the smears retrieved from the contact zone. Over 60% showed nuclear alterations. With exception to yeasts and <jats:italic toggle=\"yes\">Pseudomonas<\/jats:italic>, all microbial groups colonized the resin. No statistically significant differences were found between the oral microbiome and the acrylic resin's colonization except regarding yeasts (<jats:italic toggle=\"yes\">P<\/jats:italic> &gt; 0.05).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>Cellular alterations were found but a diagnosis of inflammation is inconclusive. Microbial adhesion to the acrylic resin was substantial, with multiple species adhering.<\/jats:p>\n          <\/jats:sec>","DOI":"10.4103\/ccd.ccd_141_18","type":"journal-article","created":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T10:37:57Z","timestamp":1675939077000},"page":"400-405","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":8,"title":["In situ Multispecies Colonization of An Acrylic Resin: Comparison to Oral Microbiome and Potential for Inflammatory Response"],"prefix":"10.4103","volume":"9","author":[{"given":"Ant\u00f3nio H. S.","family":"Delgado","sequence":"first","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o Interdisciplinar Egas Moniz, Caparica, Portugal"}]},{"given":"Joana","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o Interdisciplinar Egas Moniz, Caparica, Portugal"}]},{"given":"Gon\u00e7alo","family":"Borrecho","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o Interdisciplinar Egas Moniz, Caparica, Portugal"}]},{"given":"Teresa","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o Interdisciplinar Egas Moniz, Caparica, Portugal"}]},{"given":"Maria Eduarda","family":"Silva","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o Interdisciplinar Egas Moniz, Caparica, Portugal"}]},{"given":"S\u00e9rgio A.","family":"F\u00e9lix","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o Interdisciplinar Egas 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