{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T07:19:03Z","timestamp":1778915943055,"version":"3.51.4"},"reference-count":29,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biomark\ufffdInsights"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To measure levels of the collagen V formation marker CO5-1230 during liver fibrosis progression and regression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Monoclonal antibodies were raised against the sequence TAALGDIMGH located at the start of the C-terminal propeptide between amino acid position 1230\u2032 and 1239\u2032 (CO5-1230). An assay developed using the biotin-streptavidin system was evaluated in a rat reversible model of fibrosis. Animals were treated for duration of 4, 6 and 8 weeks. Animals that were treated for 8 weeks were left to regress for a period of 14, 20 and 26 weeks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Mean CO5-1230 level for control animals was found to be 8.7 ng\/mL. CO5-1230 marker levels, at termination points, for CCl\n                      <jats:sub>4<\/jats:sub>\n                      treated animals was be 8.7 ng\/mL at 4 weeks ( P &lt; 0.05, ROC: 0.83), 11.4 ng\/mL at 6 weeks ( P &lt; 0.001, ROC: 0.93) and 10.8 ng\/mL at 8 weeks ( P &lt; 0.05, ROC: 0.82). During regression phase, marker levels were statistically significantly decreased when compared with the marker levels at 8 weeks of treatment. Marker levels were found to be 5.9 ng\/mL ( P &lt; 0.001, ROC: 0.8) after 14 weeks of regression, 3.9 ng\/mL ( P &lt; 0.001, ROC: 0.95) after 20 weeks and 4.5 ng\/mL ( P &lt; 0.001, ROC: 0.97) after 26 weeks of regression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The data indicates that CO5-1230 levels are statistically significantly increased when CCl\n                      <jats:sub>4<\/jats:sub>\n                      intoxication stimulus is applied in all treatment time points. CO5-1230 levels return back to control levels when the stimulus is removed. The above finding adds to our previous evaluation of the marker and suggests that CO5-1230 may be a promising potential marker for liver fibrosis staging and monitoring in both disease progression and regression.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4137\/bmi.s10975","type":"journal-article","created":{"date-parts":[[2012,12,17]],"date-time":"2012-12-17T18:14:47Z","timestamp":1355768087000},"page":"BMI.S10975","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Circulating Levels of a Collagen Type V Propeptide Fragment in a Carbon Tetrachloride Reversible Model of Liver Fibrosis"],"prefix":"10.4137","volume":"7","author":[{"given":"E.","family":"Vassiliadis","sequence":"first","affiliation":[{"name":"Nordic Bioscience A\/S, Herlev, Denmark."},{"name":"University of Southern Denmark, Odense, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.S.","family":"Veidal","sequence":"additional","affiliation":[{"name":"Nordic Bioscience A\/S, Herlev, Denmark."},{"name":"University of Southern Denmark, Odense, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Nordic Bioscience A\/S, Herlev, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.A.","family":"Karsdal","sequence":"additional","affiliation":[{"name":"Nordic Bioscience A\/S, Herlev, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.J.","family":"Leeming","sequence":"additional","affiliation":[{"name":"Nordic Bioscience A\/S, Herlev, 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