{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T17:08:34Z","timestamp":1769101714377,"version":"3.49.0"},"reference-count":16,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T00:00:00Z","timestamp":1314835200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Anim. Sci."],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:p> Habibi, A., Hosseini, A., Farrokhi, N., Amidi, F., Carvalhais, I., Chaveiro, A. and Moreira da Silva, F. 2011. Short Communication: Successful vitrification of mouse oocytes using the cryotop method with moderate cryoprotectant concentrations. Can. J. Anim. Sci. 91: 385\u2013388. The response of vitrified mouse M<jats:sub>II<\/jats:sub> oocytes in the presence of two concentrations of cryoprotectants [vit<jats:sub>1<\/jats:sub> (15%: 7.5% dimethyl sulfoxide (DMSO)+7.5% ethylene glycol (EG) and vit<jats:sub>2<\/jats:sub> (30%: 15% DMSO+15% EG)] was analyzed to investigate whether reducing cryoprotectant concentrations can affect oocyte survival after cryopreservation by the cryotop method. After thawing the survival, fertilization, cleavage and blastocyst rates were compared with unfrozen oocytes. It can be concluded that 15% cryoprotectant (7.5% DMSO+7.5% EG), instead of the commonly used 30% (15% DMSO+15% EG), could be helpful by moderating the probable toxic effects of vitrification solution in mouse oocyte during vitrification by cryotop. <\/jats:p>","DOI":"10.4141\/cjas10042","type":"journal-article","created":{"date-parts":[[2011,8,16]],"date-time":"2011-08-16T10:04:21Z","timestamp":1313489061000},"page":"385-388","source":"Crossref","is-referenced-by-count":1,"title":["Short Communication: Successful vitrification of mouse oocytes using the cryotop method with moderate cryoprotectant concentrations"],"prefix":"10.1139","volume":"91","author":[{"given":"Afrooz","family":"Habibi","sequence":"first","affiliation":[{"name":"Department of Anatomical Sciences, International branch of Medical School of Shiraz University of Medical Sciences and Health Services, Kish Island, Iran"},{"name":"Animal Reproduction, Department of Agrarian Sciences, University of the Azores, Angra do Hero\u00edsmo, Portugal"}]},{"given":"Ahmad","family":"Hosseini","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Medical School of Tehran University of Medical Sciences and Health Services, Tehran, Iran"}]},{"given":"Naser","family":"Farrokhi","sequence":"additional","affiliation":[{"name":"Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran"}]},{"given":"Fardin","family":"Amidi","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Medical School of Tehran University of Medical Sciences and Health Services, Tehran, Iran"}]},{"given":"Isabel","family":"Carvalhais","sequence":"additional","affiliation":[{"name":"Animal Reproduction, Department of Agrarian Sciences, University of the Azores, Angra do Hero\u00edsmo, Portugal"}]},{"given":"Ant\u00f3nio","family":"Chaveiro","sequence":"additional","affiliation":[{"name":"Animal Reproduction, Department of Agrarian Sciences, University of the Azores, Angra do Hero\u00edsmo, Portugal"}]},{"given":"Fernando","family":"Moreira da Silva","sequence":"additional","affiliation":[{"name":"Animal Reproduction, Department of Agrarian Sciences, University of the Azores, Angra do Hero\u00edsmo, Portugal"}]}],"member":"155","reference":[{"key":"CIT0001\/CIT0001_1","doi-asserted-by":"crossref","first-page":"2884","DOI":"10.1093\/humrep\/deh504","volume":"19","author":"Hiraoka K.","year":"2004","journal-title":"Hum. 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