{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T20:50:19Z","timestamp":1789505419748,"version":"build-2803163510"},"reference-count":30,"publisher":"Korean Cancer Association","issue":"2","license":[{"start":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T00:00:00Z","timestamp":1665705600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2021R1A2C4002158"],"award-info":[{"award-number":["2021R1A2C4002158"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2022R1A2C2006322"],"award-info":[{"award-number":["2022R1A2C2006322"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003621","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["e-crt.org"],"crossmark-restriction":true},"short-container-title":["Cancer Res Treat"],"published-print":{"date-parts":[[2023,4,15]]},"abstract":"<jats:p>Purpose NUT carcinoma (NC) is a solid tumor caused by the rearrangement of &lt;i&gt;NUTM1&lt;\/i&gt; that usually develops in midline structures, such as the thorax. No standard treatment has been established despite high lethality. Thus, we investigated whether targeting the junction region of &lt;i&gt;NUTM1&lt;\/i&gt; fusion breakpoints could serve as a potential treatment option for NC. Materials and Methods We designed and evaluated a series of small interfering RNAs (siRNAs) targeting the junction region of &lt;i&gt;BRD4-NUTM1&lt;\/i&gt; fusion (&lt;i&gt;B4N&lt;\/i&gt;), the most common form of &lt;i&gt;NUTM1&lt;\/i&gt; fusion. Droplet digital polymerase chain reaction using the blood of patients was also tested to evaluate the treatment responses by the junction sequence of the &lt;i&gt;B4N&lt;\/i&gt; fusion transcripts.Results As expected, the majority of NC fusion types were &lt;i&gt;B4N&lt;\/i&gt; (12 of 18, 67%). &lt;i&gt;B4N&lt;i&gt; fusion-specific siRNA treatment on NC cells showed specific inhibitory effects on the &lt;i&gt;B4N&lt;\/i&gt; fusion transcript and fusion protein without affecting the endogenous expression of the parent genes, resulting in decreased relative cell growth and attenuation of tumor size. In addition, the fusion transcript levels in platelet-rich-plasma samples of the NC patients with systemic metastasis showed a negative correlation with therapeutic effect, suggesting its potential as a measure of treatment responsiveness.Conclusion This study suggests that tumor-specific sequences could be used to treat patients with fusion genes as part of precision medicine for a rare but deadly disease.<\/jats:p>","DOI":"10.4143\/crt.2022.910","type":"journal-article","created":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T06:23:10Z","timestamp":1665728590000},"page":"452-467","update-policy":"https:\/\/doi.org\/10.4143\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Cancer-Specific Sequences in the Diagnosis and Treatment of NUT Carcinoma"],"prefix":"10.4143","volume":"55","author":[{"given":"Mi-Sook","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sungbin","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Young","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minjung","family":"Sung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyungsoo","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Sol","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juyoung","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doo-Yi","family":"Oh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon Kyung","family":"Jeon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hobin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaithanya","family":"Lakshmi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sehhoon","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joungho","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se-Hoon","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon-La","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2954","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.12727"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2018-0477"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1097\/jto.0b013e3182a00f41"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1093\/jncics\/pkz094"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.30242"},{"key":"ref6","first-page":"1243","volume-title":"The 2015 World Health Organization classification of lung tumors: impact of genetic, clinical and radiologic advances since the 2004 classification","author":"Travis","year":"2015"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1080\/15384047.2018.1523852"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-018-0915-9"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.08.017"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.126"},{"key":"ref11","first-page":"304","volume-title":"BRD4-NUT fusion oncogene: a novel mechanism in aggressive carcinoma","author":"French","year":"2003"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.08.069"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-020-01625-0"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.can-10-3513"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1702086114"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-020-01077-z"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/bs.acr.2016.04.001"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.cd-15-1335"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1200\/jco.2018.78.2292"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1093\/jncics\/pkz093"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2019.07.006"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-0207-x"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1517039112"},{"key":"ref24","first-page":"vdaa132.","volume-title":"RNAi technology targeting the FGFR3-TACC3 fusion breakpoint: an opportunity for precision medicine","author":"Parker Kerrigan","year":"2020"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdw686"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1111\/1759-7714.13648"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800761"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-020-00484-3"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000001187"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000001021"}],"container-title":["Cancer Research and Treatment"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/e-crt.org\/upload\/pdf\/crt-2022-910.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/e-crt.org\/journal\/view.php?doi=10.4143\/crt.2022.910","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/e-crt.org\/upload\/pdf\/crt-2022-910.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T23:46:34Z","timestamp":1681343194000},"score":1,"resource":{"primary":{"URL":"http:\/\/e-crt.org\/journal\/view.php?doi=10.4143\/crt.2022.910"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,15]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,4]]}},"URL":"https:\/\/doi.org\/10.4143\/crt.2022.910","relation":{},"ISSN":["1598-2998","2005-9256"],"issn-type":[{"value":"1598-2998","type":"print"},{"value":"2005-9256","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,15]]},"assertion":[{"value":"2022-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-14","order":2,"name":"published","label":"Published online","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-15","order":3,"name":"published","label":"Print publication","group":{"name":"publication_history","label":"Publication History"}}]}}