{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T09:07:34Z","timestamp":1783933654940,"version":"3.55.0"},"reference-count":42,"publisher":"Korean Cancer Association","issue":"3","license":[{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"name":"Technology Development Program","award":["S2638360"],"award-info":[{"award-number":["S2638360"]}]},{"DOI":"10.13039\/501100013129","name":"Ministry of SMEs and Startups","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS-2024-00337984"],"award-info":[{"award-number":["RS-2024-00337984"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["e-crt.org"],"crossmark-restriction":true},"short-container-title":["Cancer Res Treat"],"published-print":{"date-parts":[[2026,7,15]]},"abstract":"<jats:p>Purpose Recent studies have revealed a diverse gastric microbiota beyond Helicobacter pylori, suggesting a role in gastric cancer (GC). We aimed to investigate the composition and characteristics of the microbiota in GC and non-cancerous gastric mucosa (NC), with a particular focus on their relationship to molecular subtypes.Materials and Methods We conducted 16S rRNA sequencing and whole transcriptomic analysis on fresh-frozen GC and NC tissue samples from 192 GC patients, as well as saliva samples from 12 GC patients and 18 healthy individuals. Microsatellite instability (MSI), Epstein-Barr virus (EBV) &lt;i&gt;in situ&lt;\/i&gt; hybridization, and immunohistochemistry for p53 and E-cadherin were used to define molecular subtypes.Results GC tissues exhibited significantly higher diversity compared to matched NC tissues, with microbial profiles marked by decreased &lt;i&gt;Helicobacter&lt;\/i&gt; and increased &lt;i&gt;Streptococcus, Prevotella&lt;\/i&gt;, and &lt;i&gt;Lactobacillus&lt;\/i&gt;. Saliva samples predominantly contained oral bacteria and exhibited distinct microbial profiles from gastric tissues. In GC tissue, &lt;i&gt;Helicobacter&lt;\/i&gt; abundance was negatively correlated with key immune checkpoint genes (&lt;i&gt;CTLA-4, PDCD1, CD274&lt;\/i&gt;, and &lt;i&gt;LAG3&lt;\/i&gt;), whereas &lt;i&gt;Prevotella, Streptococcus&lt;\/i&gt;, and &lt;i&gt;Fusobacterium&lt;\/i&gt; were positively correlated. MSI-high and EBV-positive subtypes showed lower levels of &lt;i&gt;Helicobacter&lt;\/i&gt; but higher levels of &lt;i&gt;Lactobacillus, Prevotella&lt;\/i&gt;, and &lt;i&gt;Streptococcus&lt;\/i&gt; compared to the epithelial-mesenchymal transition\u2013like subtype. Notably, within MSI-high GC, a subgroup characterized by &lt;i&gt;Lactobacillus&lt;\/i&gt;-enriched and otherwise microbiota-depleted profiles was significantly associated with poorer overall and disease-free survival.Conclusion These findings underscore distinct microbial patterns across GC molecular subtypes, suggesting potential biomarkers for GC diagnosis and treatment.<\/jats:p>","DOI":"10.4143\/crt.2025.449","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T06:32:08Z","timestamp":1765780328000},"page":"824-845","update-policy":"https:\/\/doi.org\/10.4143\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microbial Dynamics across Molecular Subtypes and Prognostic Significance of Lactobacillus in Gastric Cancer"],"prefix":"10.4143","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4372-1516","authenticated-orcid":true,"given":"Soo Kyung","family":"Nam","sequence":"first","affiliation":[{"name":"Interdisciplinary Program in Cancer Biology, Seoul National University College of Medicine, Seoul, Korea"},{"name":"Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juhyeong","family":"Park","sequence":"additional","affiliation":[{"name":"Seoul National University College of Medicine, Seoul, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujin","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoonjin","family":"Kwak","sequence":"additional","affiliation":[{"name":"Department of Pathology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheol Min","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoung Un","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nak-Jung","family":"Kwon","sequence":"additional","affiliation":[{"name":"Macrogen Inc., Seoul, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong-Ho","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Surgery, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do Joong","family":"Park","sequence":"additional","affiliation":[{"name":"Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea"},{"name":"Department of Surgery, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyuk-Joon","family":"Lee","sequence":"additional","affiliation":[{"name":"Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea"},{"name":"Department of Surgery, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Kwang","family":"Yang","sequence":"additional","affiliation":[{"name":"Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea"},{"name":"Department of Surgery, Seoul National 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