{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:27:10Z","timestamp":1780493230233,"version":"3.54.1"},"reference-count":0,"publisher":"Stichting Nase","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rhin"],"published-print":{"date-parts":[[2010,12,1]]},"abstract":"<jats:p>BACKGROUND: Human nasal epithelial cells (hNECs) are the first line of immune defense and are able to produce mediators that recruit, activate and prolong survival of immune cells, among which IL-8 takes an important place. Studies on IL-8 and effects of dexamethasone on hNECs have been hampered by methodological shortcomings. The purpose of the study is to investigate the contribution of freshly isolated hNECs to IL-8 production in chronic rhinosinusitis with nasal polyps (CRSwithNP). Secondly, the effects of dexamethasone treatment on hNEC apoptosis and IL-8 production are investigated. METHODOLOGY: hNECs were freshly isolated from nasal polyp tissue and healthy inferior turbinate of NP patients (n=12) and from inferior turbinates of healthy donors (n=19) by protease treatment and two negative selection procedures. hNECs were incubated with IL-1\u03b2 (10ng\/ml), TNF\u03b1 (10ng\/ml) or dexamethasone (10, 100 and 1000 Amicrog\/ml). After 24h, IL-8 levels were determined in the supernatants by ELISA. Finally, hNECs were incubated with increasing doses of dexamethasone and stained with trypan-blue and annexin-FITC\/PI to study apoptosis. RESULTS: hNECs isolated from nasal turbinates of healthy and NP patients and polyp tissue from NP patients produced similar levels of IL-8. IL-1\u03b2 induced higher levels of IL-8 production in all types of hNECs without differences between control and NP tissue. Dexamethasone induced apoptosis of hNECs concomitant with abrogation of IL-8 production by hNECs. CONCLUSIONS: IL-8 production by human nasal epithelial cells does not differ between NP and healthy tissue under baseline nor stimulatory conditions. Dexamethasone induces apoptosis of hNECs and abrogates IL-8 production.<\/jats:p>","DOI":"10.4193\/rhino10.033","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T08:45:32Z","timestamp":1621932332000},"page":"401-407","source":"Crossref","is-referenced-by-count":15,"title":["Dexamethasone-induced apoptosis of freshly isolated human nasal epithelial cells concomitant with abrogation of IL-8 production"],"prefix":"10.4193","volume":"48","author":[{"given":"S.","family":"Bobic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.M.","family":"van Drunen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Callebaut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Hox","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Jorissen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.J.","family":"Fokkens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.W.","family":"Hellings","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2708","container-title":["Rhinology journal"],"original-title":[],"deposited":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T08:45:33Z","timestamp":1621932333000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.rhinologyjournal.com\/Abstract.php?id=915"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,12,1]]}},"URL":"https:\/\/doi.org\/10.4193\/rhino10.033","relation":{},"ISSN":["0300-0729"],"issn-type":[{"value":"0300-0729","type":"print"}],"subject":[],"published":{"date-parts":[[2010,12,1]]}}}