{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T13:37:23Z","timestamp":1777556243912,"version":"3.51.4"},"reference-count":33,"publisher":"Genetics and Molecular Research","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genet. Mol. Res."],"DOI":"10.4238\/vol10-3gmr1334","type":"journal-article","created":{"date-parts":[[2011,7,19]],"date-time":"2011-07-19T14:06:51Z","timestamp":1311084411000},"page":"1445-1454","source":"Crossref","is-referenced-by-count":6,"title":["Cloning of the nptII gene of Escherichia coli and construction of a recombinant strain harboring functional recA and nptII antibiotic resistance"],"prefix":"10.4238","volume":"10","author":[{"given":"S.","family":"Ghanem","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2758","published-online":{"date-parts":[[2011]]},"reference":[{"issue":"Pt 1","key":"ref1","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1101\/SQB.1981.045.01.019","article-title":"Structural analysis of Tn5.","volume":"45","author":"Auerswald","year":"1981","journal-title":"Cold Spring Harb. Symp. Quant. Biol.","ISSN":"https:\/\/id.crossref.org\/issn\/0091-7451","issn-type":"print"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(82)90023-3"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.9.3628"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1574-6941.1996.tb00199.x","article-title":"Spread and evolution of natural plasmids harboring transposon Tn5.","volume":"19","author":"Bl\u00e1zquez","year":"2006","journal-title":"FEMS Microbiol. Ecol.","ISSN":"https:\/\/id.crossref.org\/issn\/0168-6496","issn-type":"print"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/B978-0-409-90042-2.50016-7","article-title":"Expression Vectors Employing Lambda-, trp-, lac- and lpp-Derived Promoters.","volume-title":"Vectors: A Survey of Molecular Cloning Vectors and Their Uses","author":"Brosius","year":"1988"},{"key":"ref6","doi-asserted-by":"crossref","DOI":"10.5962\/bhl.title.84653","volume-title":"Manual of Tropical Medicine.","author":"Castellani","year":"1919","edition":"3rd edn."},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.20.9677"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1128\/JB.151.2.924-931.1982","article-title":"Isolation of the kanamycin resistance region (Tn2350) of plasmid R1drd-19 as an autonomous replicon.","volume":"151","author":"Clerget","year":"1982","journal-title":"J. Bacteriol.","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9193","issn-type":"print"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(81)90321-1"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1006\/mben.1999.0131"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-008-9900-6"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1128\/jb.170.3.1275-1278.1988","article-title":"A clinical isolate of transposon Tn5 expressing streptomycin resistance in Escherichia coli.","volume":"170","author":"Genilloud","year":"1988","journal-title":"J. Bacteriol.","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9193","issn-type":"print"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007\/BF00270890"},{"key":"ref14","first-page":"4635","article-title":"Plasmid profile analysis of multidrug resistant E. coli isolated from UTI patients of Nagpur City, India.","volume":"14","author":"Jan","year":"2009","journal-title":"Romanian Biotechnol. Lett."},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038\/287869a0"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1086\/653932"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/165637"},{"key":"ref18","article-title":"Purification of PCR Products.","volume-title":"PCR Technology: Current. Innovations","volume":"21","author":"Mezei","year":"1994"},{"key":"ref19","article-title":"Introduction to Biotechniques.","volume-title":"PCR","volume":"13","author":"Newton","year":"1994"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(79)90044-1"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"3317","DOI":"10.1002\/j.1460-2075.1984.tb02297.x","article-title":"Protein fusions with the kanamycin resistance gene from transposon Tn5.","volume":"3","author":"Reiss","year":"1984","journal-title":"EMBO J.","ISSN":"https:\/\/id.crossref.org\/issn\/0261-4189","issn-type":"print"},{"key":"ref22","first-page":"19","article-title":"pGEM\u00ae-T Vector Systems Troubleshooting Guide. Technical Manual.","volume":"45","author":"Robles","year":"1994","journal-title":"Promega Notes"},{"issue":"Pt 1","key":"ref23","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1101\/SQB.1981.045.01.018","article-title":"Genetic organization of Tn5.","volume":"45","author":"Rothstein","year":"1981","journal-title":"Cold Spring Harb. Symp. Quant. Biol.","ISSN":"https:\/\/id.crossref.org\/issn\/0091-7451","issn-type":"print"},{"key":"ref24","volume-title":"Molecular Cloning: A Laboratory Manual.","author":"Sambrook","year":"1989"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200202143460717"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1042\/bj2680671","article-title":"Cloning of aminoglycoside phosphotransferase (APH) gene from antibiotic-producing strain of Bacillus circulans into a high-expression vector, pKK223-3. Purification, properties and location of the enzyme.","volume":"268","author":"Sarwar","year":"1990","journal-title":"Biochem. J.","ISSN":"https:\/\/id.crossref.org\/issn\/0264-6021","issn-type":"print"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.1128\/AAC.39.8.1790","article-title":"Cloning and characterization of a 3-N-aminoglycoside acetyltransferase gene, aac(3)-Ib, from Pseudomonas aeruginosa.","volume":"39","author":"Schwocho","year":"1995","journal-title":"Antimicrob. Agents Chemother.","ISSN":"https:\/\/id.crossref.org\/issn\/0066-4804","issn-type":"print"},{"key":"ref28","first-page":"915","article-title":"Cloning of aminoglycoside-resistance determinants from Streptomyces tenebrurius and comparison with related genes from other actinomycetes.","volume":"133","author":"Skeggs","year":"1987","journal-title":"J. Gen. Microbiol.","ISSN":"https:\/\/id.crossref.org\/issn\/0022-1287","issn-type":"print"},{"key":"ref29","first-page":"327","article-title":"Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.","volume":"1","author":"Southern","year":"1982","journal-title":"J. Mol. Appl. Genet.","ISSN":"https:\/\/id.crossref.org\/issn\/0271-6801","issn-type":"print"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1128\/AAC.32.8.1170","article-title":"Detection of two different kanamycin resistance genes in naturally occurring isolates of Campylobacter jejuni and Campylobacter coli.","volume":"32","author":"Tenover","year":"1988","journal-title":"Antimicrob. Agents Chemother.","ISSN":"https:\/\/id.crossref.org\/issn\/0066-4804","issn-type":"print"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(90)90463-2"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.9.3469"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"2439","DOI":"10.1128\/AAC.41.11.2439","article-title":"Cloning and characterization of an aminoglycoside 6\u2019-N-acetyltransferase gene from Citrobacter freundii which confers an altered resistance profile.","volume":"41","author":"Wu","year":"1997","journal-title":"Antimicrob. Agents Chemother.","ISSN":"https:\/\/id.crossref.org\/issn\/0066-4804","issn-type":"print"}],"container-title":["Genetics and Molecular Research"],"original-title":[],"link":[{"URL":"http:\/\/www.funpecrp.com.br\/gmr\/year2011\/vol10-3\/pdf\/gmr1334.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,21]],"date-time":"2020-06-21T12:11:20Z","timestamp":1592741480000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.funpecrp.com.br\/gmr\/year2011\/vol10-3\/pdf\/gmr1334.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011]]},"references-count":33,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2011]]}},"URL":"https:\/\/doi.org\/10.4238\/vol10-3gmr1334","relation":{},"ISSN":["1676-5680"],"issn-type":[{"value":"1676-5680","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011]]}}}