{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T19:19:37Z","timestamp":1793215177646,"version":"4.5.0"},"reference-count":28,"publisher":"Baishideng Publishing Group Inc.","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World J Gastrointest Oncol"],"abstract":"<jats:p>BACKGROUND<\/jats:p>\n          <jats:p>RAS , BRAF , and mismatch repair (MMR)\/microsatellite instability (MSI) are crucial biomarkers recommended by clinical practice guidelines for colorectal cancer (CRC). However, their characteristics and influencing factors in Chinese patients have not been thoroughly described.<\/jats:p>\n          <jats:p>AIM<\/jats:p>\n          <jats:p>To analyze the clinicopathological features of KRAS , NRAS , BRAF , and PIK3CA  mutations and the DNA MMR status in CRC.<\/jats:p>\n          <jats:p>METHODS<\/jats:p>\n          <jats:p>We enrolled 2271 Chinese CRC patients at the China-Japan Friendship Hospital. MMR proteins were tested using immunohistochemical analysis, and the KRAS\/NRAS\/BRAF\/PIK3CA  mutations were determined using quantitative polymerase chain reaction. Microsatellite status was determined using an MSI detection kit. Statistical analyses were conducted using SPSS software and logistic regression.<\/jats:p>\n          <jats:p>RESULTS<\/jats:p>\n          <jats:p>The KRAS , NRAS , BRAF , and PIK3CA  mutations were detected in 44.6%, 3.4%, 3.7%, and 3.9% of CRC patients, respectively. KRAS  mutations were more likely to occur in patients with moderate-to-high differentiation. BRAF  mutations were more likely to occur in patients with right-sided CRC, poorly differentiated, or no perineural invasion. Deficient MMR (dMMR) was detected in 7.9% of all patients and 16.8% of those with mucinous adenocarcinomas. KRAS , NRAS , BRAF , and PIK3CA  mutations were detected in 29.6%, 1.1%, 8.1%, and 22.3% of patients with dMMR, respectively. The dMMR was more likely to occur in patients with a family history of CRC, aged &lt; 50 years, right-sided CRC, poorly differentiated histology, no perineural invasion, and with carcinoma in situ , stage I, or stage II tumors.<\/jats:p>\n          <jats:p>CONCLUSION<\/jats:p>\n          <jats:p>This study analyzed the molecular profiles of KRAS , NRAS , BRAF , PIK3CA , and MMR\/MSI in CRC, identifying key influencing factors, with implications for clinical management of CRC.<\/jats:p>","DOI":"10.4251\/wjgo.v16.i6.2673","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T07:43:34Z","timestamp":1718351014000},"page":"2673-2682","source":"Crossref","is-referenced-by-count":6,"title":["Comprehensive analysis of gene mutations and mismatch repair in Chinese colorectal cancer patients"],"prefix":"10.4251","volume":"16","author":[{"given":"Huang","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-Ying","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhan","family":"Hua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Li","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian-Qian","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Ning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Fen","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da-Kui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Zheng","family":"Jie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ding-Rong","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2060","published-online":{"date-parts":[[2024,6,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-019-0209-8"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.cn112152-20220922-00647"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1200\/JOP.2017.022152"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdx052"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.29855"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdx738"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2011.645805"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1207756"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2016.10.005"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00396"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-019-0126-x"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.27.1825"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.00096"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.suronc.2018.05.012"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.12998\/wjcc.v6.i15.869"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.2217\/fon-2020-1055"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1007\/s10350-004-0932-1"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1186\/s40880-019-0361-0"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-13-135"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdq258"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-015-1276-z"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.23750\/abm.v89i9-S.7960"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-017-1376-4"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet-2011-100714"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1111\/apm.13091"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.1007"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.32489"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1007\/s11864-015-0348-2"}],"container-title":["World Journal of Gastrointestinal Oncology"],"original-title":[],"link":[{"URL":"https:\/\/www.wjgnet.com\/1948-5204\/full\/v16\/i6\/2673.htm","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.wjgnet.com\/1948-5204\/full\/v16\/i6\/2673.htm","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T07:46:13Z","timestamp":1718351173000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.wjgnet.com\/1948-5204\/full\/v16\/i6\/2673.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,15]]},"references-count":28,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.4251\/wjgo.v16.i6.2673","relation":{},"ISSN":["1948-5204"],"issn-type":[{"value":"1948-5204","type":"print"}],"subject":[],"published":{"date-parts":[[2024,6,15]]}}}