{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:51:16Z","timestamp":1791917476619,"version":"4.3.4"},"reference-count":43,"publisher":"Baishideng Publishing Group Inc.","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World J Stem Cells"],"abstract":"BACKGROUND<\/jats:p>\n Pulmonary fibrosis is one of the main reasons for the high mortality rate among acute respiratory distress syndrome (ARDS) patients. Mesenchymal stromal cell-derived microvesicles (MSC-MVs) have been shown to exert antifibrotic effects in lung diseases.<\/jats:p>\n AIM<\/jats:p>\n To investigate the effects and mechanisms of MSC-MVs on pulmonary fibrosis in ARDS mouse models.<\/jats:p>\n METHODS<\/jats:p>\n MSC-MVs with low hepatocyte growth factor (HGF) expression (siHGF-MSC-MVs) were obtained via lentivirus transfection and used to establish the ARDS pulmonary fibrosis mouse model. Following intubation, respiratory mechanics-related indicators were measured via an experimental small animal lung function tester. Homing of MSC-MVs in lung tissues was investigated by near-infrared live imaging. Immunohistochemical, western blotting, ELISA and other methods were used to detect expression of pulmonary fibrosis-related proteins and to compare effects on pulmonary fibrosis and fibrosis-related indicators.<\/jats:p>\n RESULTS<\/jats:p>\n The MSC-MVs gradually migrated and homed to damaged lung tissues in the ARDS model mice. Treatment with MSC-MVs significantly reduced lung injury and pulmonary fibrosis scores. However, low expression of HGF (siHGF-MSC-MVs) significantly inhibited the effects of MSC-MVs (P < 0.05). Compared with the ARDS pulmonary fibrosis group, the MSC-MVs group exhibited suppressed expression of type I collagen antigen, type III collagen antigen, and the proteins transforming growth factor-\u03b2 and \u03b1-smooth muscle actin, whereas the siHGF-MVs group exhibited significantly increased expression of these proteins. In addition, pulmonary compliance and the pressure of oxygen\/oxygen inhalation ratio were significantly lower in the MSC-MVs group, and the effects of the MSC-MVs were significantly inhibited by low HGF expression (all P < 0.05).<\/jats:p>\n CONCLUSION<\/jats:p>\n MSC-MVs improved lung ventilation functions and inhibited pulmonary fibrosis in ARDS mice partly via HGF mRNA transfer.<\/jats:p>","DOI":"10.4252\/wjsc.v16.i8.811","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T02:13:30Z","timestamp":1724638410000},"page":"811-823","source":"Crossref","is-referenced-by-count":6,"title":["Microvesicles derived from mesenchymal stem cells inhibit acute respiratory distress syndrome-related pulmonary fibrosis in mouse partly through hepatocyte growth factor"],"prefix":"10.4252","volume":"16","author":[{"given":"Qi-Hong","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng-Lei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Na","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Mei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2060","published-online":{"date-parts":[[2024,8,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)01439-8"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10040756"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-020-06299-6"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000002046"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202202-0399ST"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2023.114537"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-022-02822-2"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2022.0327"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2020.1748940"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1007\/s13577-021-00525-z"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2022.1039121"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21239143"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.600711"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S344309"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.808855"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22041790"},{"key":"B17","unstructured":"Yunus J, Salman M, Lintin GBR, Muchtar M, Sari DCR, Arfian N, Romi MM. 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