{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T06:56:27Z","timestamp":1788332187741,"version":"build-2803163510"},"reference-count":37,"publisher":"Baishideng Publishing Group Inc.","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World J Stem Cells"],"abstract":"<jats:p>BACKGROUND<\/jats:p>\n          <jats:p>Non-small cell lung cancer (NSCLC) is the most prevalent subtype of lung cancer, accounting for approximately 85% of all lung cancer cases and remaining a major cause of cancer-related mortality worldwide. Despite advances in diagnostic and therapeutic approaches, the incidence and mortality rates of NSCLC continue to rise, especially in low-income and middle-income countries.<\/jats:p>\n          <jats:p>AIM<\/jats:p>\n          <jats:p>To investigate the expression of cancer stem cell (CSC) markers and their relationship with the prognosis and survival of patients with stage IIIA NSCLC.<\/jats:p>\n          <jats:p>METHODS<\/jats:p>\n          <jats:p>A retrospective analysis was conducted on the clinical data and survival follow-up information of 61 patients with stage IIIA NSCLC treated at our hospital from February 2020 to June 2022, and all cases were confirmed as primary (non-recurrent) diagnoses based on clinical and pathological records. All patients were followed up through outpatient visits or telephone interviews. The follow-up duration ranged from 6 to 51 months with a median follow-up time of 36 months. Overall survival (OS) was defined as the time from the date of pathological diagnosis to death or the last follow-up. Univariate and multivariate Cox regression analyses were performed to examine the relationship between clinical characteristics and OS. Immunohistochemistry was used to detect the expression of CSC markers [octamer-binding transcription factor 4 (OCT4), trophoblast cell surface antigen-2 (TROP-2), ATP-binding cassette subfamily G member 2 (ABCG2), p75 neurotrophin receptor (p75NTR)] in NSCLC, followed by immunohistochemical scoring. The high H-scores of CSC markers, age, and micropapillary components were combined to generate a tumor stemness index (TSI). The Kaplan-Meier method was used to analyze the relationship between CSC markers, TSI, and OS in patients with NSCLC.<\/jats:p>\n          <jats:p>RESULTS<\/jats:p>\n          <jats:p>Multivariate Cox regression analysis showed that age [hazard ratio (HR) = 1.952, 95% confidence interval (CI): 1.087-2.481, P  = 0.029] and micropapillary components (HR = 2.716, 95%CI: 1.259-5.837, P  = 0.013) were significantly associated with OS. In NSCLC there were 21 cases with high OCT4 H-scores, 27 cases with high TROP-2 H-scores, 44 cases with high ABCG2 H-scores, and 44 cases with high p75NTR H-scores. In the survival analysis the high OCT4 expression group had a poorer prognosis (P  = 0.006). Further subtype analysis revealed no statistically significant difference in OS between high and low OCT4 H-score groups in patients with lung squamous cell carcinoma (P  = 0.457). However, in patients with lung adenocarcinoma high OCT4 expression had significantly poorer OS compared with those with low OCT4 expression (P  = 0.005). TROP-2, ABCG2, and p75NTR did not significantly affect the prognosis. TSI was significantly associated with OS in patients with NSCLC (HR = 2.209, 95%CI: 1.238-3.681, P  = 0.027).<\/jats:p>\n          <jats:p>CONCLUSION<\/jats:p>\n          <jats:p>Age and micropapillary components were related to OS in patients with stage IIIA NSCLC. High expression of OCT4 and high TSI were associated with poor prognosis.<\/jats:p>","DOI":"10.4252\/wjsc.v17.i9.106381","type":"journal-article","created":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:35:43Z","timestamp":1758674143000},"source":"Crossref","is-referenced-by-count":2,"title":["Expression of cancer stem cell markers and their prognostic significance in stage IIIA non-small cell lung cancer"],"prefix":"10.4252","volume":"17","author":[{"given":"Tao","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si-Cong","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian-Ming","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Zhen","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cai-Jin","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You-Dan","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2060","published-online":{"date-parts":[[2025,9,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.cn112152-20231024-00243"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.36740\/WLek202209109"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1007\/s11547-023-01659-w"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2020.61.7.572"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.33160\/yam.2021.02.002"},{"key":"B6","unstructured":"Dai L, Zhao X, Ma L, Li X, Zuo D, Li Y, Wei B, Sui Y, Xu F. 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