{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:25:30Z","timestamp":1762251930969},"reference-count":39,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,5,1]]},"abstract":"<jats:p><jats:italic>Plasmodium knowlesi<\/jats:italic> is now known as the fifth <jats:italic>Plasmodium<\/jats:italic> species that can cause human malaria. The <jats:italic>Plasmodium<\/jats:italic> merozoite surface protein (MSP) has been reported to be potential target for vaccination and diagnosis of malaria. MSP-1<jats:sub>33<\/jats:sub> has been shown to be immunogenic and its T cell epitopes could mediate cellular immune protection. However, limited studies have focused on <jats:italic>P. knowlesi<\/jats:italic> MSP-1<jats:sub>33<\/jats:sub>. In this study, an approximately 28-kDa recombinant <jats:italic>P. knowlesi<\/jats:italic> MSP-1<jats:sub>33<\/jats:sub> (pkMSP-1<jats:sub>33<\/jats:sub>) was expressed by using an <jats:italic>Escherichia coli<\/jats:italic> system. The purified pkMSP-1<jats:sub>33<\/jats:sub> reacted with serum samples of patients infected with <jats:italic>P. knowlesi<\/jats:italic> (31 of 31, 100%) and non-<jats:italic>P. knowlesi<\/jats:italic> malaria (27 of 28, 96.43%) by Western blotting. The pkMSP-1<jats:sub>33<\/jats:sub> also reacted with <jats:italic>P. knowlesi<\/jats:italic> (25 of 31, 80.65%) and non-<jats:italic>P. knowlesi<\/jats:italic> malaria sera (20 of 28, 71.43%) in an enzyme-linked immunosorbent assay (ELISA). Most of the non-malarial infection (49 of 52 in by Western blotting and 46 of 52 in the ELISA) and healthy donor serum samples (65 of 65 by Western blotting and ELISA) did not react with recombinant pkMSP-1<jats:sub>33<\/jats:sub>.<\/jats:p>","DOI":"10.4269\/ajtmh.12-0250","type":"journal-article","created":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T07:15:38Z","timestamp":1363677338000},"page":"835-840","source":"Crossref","is-referenced-by-count":11,"title":["Evaluation of Recombinant Plasmodium knowlesi Merozoite Surface Protein-133 for Detection of Human Malaria"],"prefix":"10.4269","volume":"88","author":[{"given":"Fei Wen","family":"Cheong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yee Ling","family":"Lau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mun Yik","family":"Fong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohela","family":"Mahmud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Immunol Cell Biol","first-page":"377","article-title":"The future for blood-stage vaccines against malaria","volume":"87","author":"Richards","year":"2009"},{"key":"R2","series-title":"Nature","first-page":"214","article-title":"The global distribution of clinical episodes of Plasmodium falciparum malaria","volume":"434","author":"Snow","year":"2005"},{"key":"R3","series-title":"Malar J","first-page":"15","article-title":"Swedish traveller with Plasmodium knowlesi malaria after visiting Malaysian Borneo","volume":"8","author":"Bronner","year":"2009"},{"key":"R4","series-title":"Lancet","first-page":"1017","article-title":"A large focus of naturally acquired Plasmodium knowlesi infections in human beings","volume":"363","author":"Singh","year":"2004"},{"key":"R5","series-title":"Emerg Infect Dis","first-page":"1476","article-title":"Co-infections with Plasmodium knowlesi and other malaria parasites, Myanmar","volume":"16","author":"Jiang","year":"2010"},{"key":"R6","series-title":"Emerg Infect Dis","first-page":"2211","article-title":"Naturally acquired Plasmodium knowlesi malaria in human, Thailand","volume":"10","author":"Jongwutiwes","year":"2004"},{"key":"R7","series-title":"Emerg Infect Dis","first-page":"811","article-title":"Human infections with Plasmodium knowlesi, the Philippines","volume":"14","author":"Luchavez","year":"2008"},{"key":"R8","series-title":"Emerg Infect Dis","first-page":"814","article-title":"Naturally acquired human Plasmodium knowlesi infection, Singapore","volume":"14","author":"Ng","year":"2008"},{"key":"R9","series-title":"Malar J","first-page":"269","article-title":"Knowlesi malaria in Vietnam","volume":"8","author":"Cox-Singh","year":"2009"},{"key":"R10","series-title":"Malar J","first-page":"249","article-title":"Human Plasmodium knowlesi infections in young children in central Vietnam","volume":"8","author":"Van den Eede","year":"2009"},{"key":"R11","series-title":"Emerg Infect Dis","first-page":"814","article-title":"Plasmodium knowlesi malaria in children","volume":"17","author":"Barber","year":"2011"},{"key":"R12","series-title":"Clin Infect Dis","first-page":"165","article-title":"Plasmodium knowlesi malaria in humans is widely distributed and potentially life threatening","volume":"46","author":"Cox-Singh","year":"2008"},{"key":"R13","series-title":"Clin Infect Dis","first-page":"852","article-title":"Clinical and laboratory features of human Plasmodium knowlesi infection","volume":"49","author":"Daneshvar","year":"2009"},{"key":"R14","series-title":"Malar J","first-page":"73","article-title":"Morphological features and differential counts of Plasmodium knowlesi parasites in naturally acquired human infections","volume":"8","author":"Lee","year":"2009"},{"issue":"(Suppl 3)","key":"R15","series-title":"Mem Inst Oswaldo Cruz","first-page":"37","article-title":"A malaria merozoite surface protein (MSP1)-structure, processing and function","volume":"87","author":"Holder","year":"1992"},{"key":"R16","series-title":"Nat Rev Immunol","first-page":"117","article-title":"Towards a blood-stage vaccine for malaria: are we following all the leads?","volume":"1","author":"Good","year":"2001"},{"key":"R17","series-title":"J Exp Med","first-page":"1528","article-title":"Biosynthesis and processing of a Plasmodium falciparum schizont antigen recognized by immune serum and a monoclonal antibody","volume":"156","author":"Holder","year":"1982"},{"key":"R18","series-title":"Mol Biochem Parasitol","first-page":"71","article-title":"Fragments of the polymorphic Mr 185,000 glycoprotein from the surface of isolated Plasmodium falciparum merozoites form an antigenic complex","volume":"23","author":"McBride","year":"1987"},{"key":"R19","series-title":"Mol Biochem Parasitol","first-page":"35","article-title":"Processing of the Plasmodium falciparum major merozoite surface protein-1: identification of a 33-kilodalton secondary processing product which is shed prior to erythrocyte invasion","volume":"49","author":"Blackman","year":"1991"},{"key":"R20","series-title":"Methods Cell Biol","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0091-679X(08)61853-1","article-title":"Purification of Plasmodium falciparum merozoites for analysis of the processing of merozoite surface protein-1","volume":"45","author":"Blackman","year":"1994"},{"key":"R21","series-title":"Mol Biochem Parasitol","first-page":"29","article-title":"Proteolytic processing of the Plasmodium falciparum merozoite surface protein-1 produces a membrane-bound fragment containing two epidermal growth factor-like domains","volume":"49","author":"Blackman","year":"1991"},{"key":"R22","series-title":"Infect Immun","first-page":"2245","article-title":"Different regions of the malaria merozoite surface protein 1 of Plasmodium chabaudi elicit distinct T-cell and antibody isotype responses","volume":"69","author":"Quin","year":"2001"},{"key":"R23","series-title":"J Immunol","first-page":"944","article-title":"Identification of T cell epitopes on the 33-kDa fragment of Plasmodium yoelii merozoite surface protein 1 and their antibody-independent protective role in immunity to blood stage malaria","volume":"169","author":"Wipasa","year":"2002"},{"key":"R24","series-title":"Cell Host Microbe","first-page":"95","article-title":"Recombinant viral vaccines expressing merozoite surface protein-1 induce antibody- and T cell-mediated multistage protection against malaria","volume":"5","author":"Draper","year":"2009"},{"key":"R25","series-title":"Infect Immun","first-page":"6075","article-title":"Merozoite surface protein 1-specific immune response is protective against exoerythrocytic forms of Plasmodium yoelii","volume":"70","author":"Kawabata","year":"2002"},{"key":"R26","series-title":"Infect Immun","first-page":"3024","article-title":"Characterization of human T- and B-cell epitopes in the C terminus of Plasmodium falciparum merozoite surface protein 1: evidence for poor T-cell recognition of polypeptides with numerous disulfide bonds","volume":"65","author":"Egan","year":"1997"},{"key":"R27","series-title":"PLoS Clin Trials","first-page":"e12","article-title":"Phase 1 study of two merozoite surface protein 1 (MSP1(42)) vaccines for Plasmodium falciparum malaria","volume":"2","author":"Malkin","year":"2007"},{"key":"R28","series-title":"Mol Ther","first-page":"2269","article-title":"Phase Ia clinical evaluation of the Plasmodium falciparum blood-stage antigen MSP1 in ChAd63 and MVA vaccine vectors","volume":"19","author":"Sheehy","year":"2011"},{"key":"R29","series-title":"J Immunol","first-page":"6022","article-title":"Identification of T and B cell epitopes recognized by humans in the C-terminal 42-kDa domain of the Plasmodium falciparum merozoite surface protein (MSP)-1","volume":"154","author":"Udhayakumar","year":"1995"},{"key":"R30","series-title":"J Chromatogr B Analyt Technol Biomed Life Sci","first-page":"61","article-title":"Expression and purification of Plasmodium falciparum MSP-1(42): a malaria vaccine candidate","volume":"786","author":"Epp","year":"2003"},{"key":"R31","series-title":"Nucleic Acids Res","first-page":"1094","article-title":"Vaccine candidate MSP-1 from Plasmodium falciparum: a redesigned 4917 bp polynucleotide enables synthesis and isolation of full-length protein from Escherichia coli and mammalian cells","volume":"27","author":"Pan","year":"1999"},{"key":"R32","series-title":"J Gen Virol","first-page":"2577","article-title":"Comparison of ELISA and Western blotting for human papillomavirus type 16 E7 antibody determination","volume":"72","author":"Suchankova","year":"1991"},{"key":"R33","series-title":"Am J Trop Med Hyg","first-page":"16","article-title":"Antigenicity, immunogenicity, and protective efficacy of Plasmodium vivax MSP1 PV200l: a potential malaria vaccine subunit","volume":"73","author":"Valderrama-Aguirre","year":"2005"},{"key":"R34","series-title":"Infect Immun","first-page":"5936","article-title":"Merozoite surface protein 1 of Plasmodium vivax induces a protective response against Plasmodium cynomolgi challenge in rhesus monkeys","volume":"73","author":"Dutta","year":"2005"},{"key":"R35","series-title":"J Parasitol","first-page":"403","article-title":"Characterization of cross-reactive blood-stage antigens of the Plasmodium cynomolgi complex using anti-Plasmodium vivax monoclonal antibodies","volume":"74","author":"Kamboj","year":"1988"},{"key":"R36","series-title":"Am J Trop Med Hyg","first-page":"365","article-title":"Immunologic characterization of Plasmodium vivax antigens using Plasmodium cynomolgi liver stage-primed immune sera","volume":"51","author":"Yang","year":"1994"},{"key":"R37","series-title":"Korean J Parasitol","first-page":"15","article-title":"A recombinant Plasmodium vivax apical membrane antigen-1 to detect human infection in Iran","volume":"50","author":"Haghi","year":"2012"},{"key":"R38","series-title":"PLoS Pathog","first-page":"e1000770","article-title":"Long-lived antibody and B cell memory responses to the human malaria parasites, Plasmodium falciparum and Plasmodium vivax","volume":"6","author":"Wipasa","year":"2010"},{"key":"R39","series-title":"Korean J Parasitol","first-page":"171","article-title":"Western blot diagnosis of vivax malaria with multiple stage-specific antigens of the parasite","volume":"39","author":"Son","year":"2001"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/88\/5\/article-p835.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/88\/5\/article-p835.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/88\/5\/article-p835.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T18:34:47Z","timestamp":1723055687000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/88\/5\/article-p835.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,1]]},"references-count":39,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.12-0250","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"type":"print","value":"0002-9637"},{"type":"electronic","value":"1476-1645"}],"subject":[],"published":{"date-parts":[[2013,5,1]]}}}