{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T16:19:40Z","timestamp":1787415580174,"version":"build-2736575974"},"reference-count":11,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,5,1]]},"abstract":"<jats:p>Inter- and intra-observer variation was examined among six microscopists who read 50 scrub typhus (ST) and murine typhus (MT) indirect immunofluorescence assay (IFA) immunoglobulin M (IgM) slides. Inter-observer agreement was moderate (\u03ba = 0.45) for MT and fair (\u03ba = 0.32) for ST, and was significantly correlated with experience (<jats:italic>P<\/jats:italic> = 0.03 and <jats:italic>P<\/jats:italic> = 0.004, respectively); \u03ba-scores for intra-observer agreement between morning and afternoon readings (range = 0.35\u20130.86) were not correlated between years of experience for ST and MT IFAs (Spearman's \u03c1 = 0.31, <jats:italic>P<\/jats:italic> = 0.54 and <jats:italic>P<\/jats:italic> = 0.14, respectively; <jats:italic>P<\/jats:italic> = 0.78). Storage at 4\u00b0C for 2 days showed a change from positive to negative in 20\u201332% of slides. Although the titers did not dramatically change after 14 days of storage, the final interpretation (positive to negative) did change in 36\u201350% of samples, and it, therefore, recommended that slides should be read as soon as possible after processing.<\/jats:p>","DOI":"10.4269\/ajtmh.12-0325","type":"journal-article","created":{"date-parts":[[2013,3,12]],"date-time":"2013-03-12T02:20:34Z","timestamp":1363054834000},"page":"932-936","source":"Crossref","is-referenced-by-count":43,"title":["Inter- and Intra-Operator Variability in the Reading of Indirect Immunofluorescence Assays for the Serological Diagnosis of Scrub Typhus and Murine Typhus"],"prefix":"10.4269","volume":"88","author":[{"given":"Rattanaphone","family":"Phetsouvanh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaksinaporn","family":"Thojaikong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phonlavanh","family":"Phoumin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bountoy","family":"Sibounheuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koukeo","family":"Phommasone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vilada","family":"Chansamouth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue 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counting","volume":"134","author":"Parkash","year":"2010"}],"container-title":["The American Society of Tropical Medicine and 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