{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T04:34:32Z","timestamp":1779338072631,"version":"3.51.4"},"reference-count":14,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,3,6]]},"abstract":"<jats:p><jats:italic>Leishmania major<\/jats:italic> is the only species of <jats:italic>Leishmania<\/jats:italic> known to cause cutaneous leishmanisis (CL) in Mali. We amplified <jats:italic>Leishmania<\/jats:italic> DNA stored on archived Giemsa-stained dermal scraping slides obtained from self-referral patients with clinically suspected CL seen in the Center National d'Appui A La Lutte Contre La Maladie (CNAM) in Bamako, Mali, to determine if any other <jats:italic>Leishmania<\/jats:italic> species were responsible for CL in Mali and evaluate its geographic distribution. Polymerase chain reaction (PCR) amplification was performed using a <jats:italic>Leishmania<\/jats:italic> species-specific primer pair that can amplify DNA from <jats:italic>L. major<\/jats:italic>, <jats:italic>L. tropica<\/jats:italic>, <jats:italic>L. infantum<\/jats:italic>, and <jats:italic>L. donovani<\/jats:italic> parasites, possible causative agents of CL in Mali. <jats:italic>L. major<\/jats:italic> was the only species detected in 41 microscopically confirmed cases of CL from five regions of Mali (Kayes, Koulikoro, S\u00e9gou, Mopti, and Tombouctou). These results implicate <jats:italic>L. major<\/jats:italic> as the predominant, possibly exclusive species responsible for CL in Mali.<\/jats:p>","DOI":"10.4269\/ajtmh.12-0434","type":"journal-article","created":{"date-parts":[[2013,1,17]],"date-time":"2013-01-17T05:43:24Z","timestamp":1358401404000},"page":"583-585","source":"Crossref","is-referenced-by-count":14,"title":["Leishmania major, the Predominant Leishmania Species Responsible for Cutaneous Leishmaniasis in Mali"],"prefix":"10.4269","volume":"88","author":[{"given":"Carlos","family":"Paz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sibiry","family":"Samake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer M.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ousmane","family":"Faye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Traore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koureishi","family":"Tall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moumine","family":"Cisse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somita","family":"Keita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesus G.","family":"Valenzuela","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seydou","family":"Doumbia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"R1","series-title":"PLoS One","first-page":"e35671","article-title":"Leishmaniasis worldwide and global estimates of its incidence","volume":"7","author":"Alvar","year":"2012"},{"key":"R2","series-title":"Trans R Soc Trop Med Hyg","first-page":"236","article-title":"Leishmaniasis in the Republic of Mali","volume":"63","author":"Imperato","year":"1969"},{"key":"R3","series-title":"Acta Trop","first-page":"260","article-title":"Leishmanin skin sensitivity in northwestern Mali","volume":"27","author":"Imperato","year":"1970"},{"key":"R4","series-title":"Trop Geogr Med","first-page":"303","article-title":"Leishmanin skin sensitivity in the inland delta of the Niger","volume":"26","author":"Imperato","year":"1974"},{"key":"R5","series-title":"Mali Med","first-page":"29","article-title":"Epidemiology and clinical polymorphism of cutaneous leishmaniasis diagnosed at CNAM (Farmerly Marchoux Institute), Bamako (Mali)","volume":"18","author":"Keita","year":"2003"},{"key":"R6","series-title":"PLoS Negl Trop Dis","first-page":"e565","article-title":"Discrepant prevalence and incidence of Leishmania infection between two neighboring villages in central Mali based on leishmanin skin test surveys","volume":"3","author":"Oliveira","year":"2009"},{"key":"R7","series-title":"Ann Parasitol Hum Comp","first-page":"93","article-title":"Presence of Leishmania major Yakimoff and Schokhor, 1914 in Mali. Enzymatic identification of a strain of human origin","volume":"60","author":"Garin","year":"1985"},{"key":"R8","series-title":"Ann Parasitol Hum Comp","first-page":"510","article-title":"Presence of Leishmania major MON-26 in Mali","volume":"64","author":"Izri","year":"1989"},{"key":"R9","series-title":"Trop Med Int Health","first-page":"1071","article-title":"Geographical distribution and epidemiological features of Old World cutaneous leishmaniasis foci, based on the isoenzyme analysis of 1048 strains","volume":"14","author":"Pratlong","year":"2009"},{"key":"R10","series-title":"Ann Trop Med Parasitol","first-page":"31","article-title":"DNA extraction and amplification of Leishmania from archived, Giemsa-stained slides, for the diagnosis of cutaneous leishmaniasis by PCR","volume":"96","author":"Motazedian","year":"2002"},{"key":"R11","series-title":"Clin Microbiol Infect","first-page":"815","article-title":"Leishmania identification by PCR of Giemsa-stained lesion imprint slides stored for up to 36 years","volume":"12","author":"Volpini","year":"2006"},{"issue":"(Suppl 1)","key":"R12","series-title":"Trans R Soc Trop Med Hyg","first-page":"S87","article-title":"Distinguishing Leishmania tropica and Leishmania major in the Middle East using the polymerase chain reaction with kinetoplast DNA-specific primers","volume":"96","author":"Anders","year":"2002"},{"key":"R13","series-title":"PLoS Negl Trop Dis","first-page":"e1139","article-title":"Seasonality and prevalence of Leishmania major infection in Phlebotomus duboscqi Neveu-Lemaire from two neighboring villages in central Mali","volume":"10","author":"Anderson","year":"2011"},{"key":"R14","series-title":"J Clin Microbiol","first-page":"1435","article-title":"Comparison of PCR assays for diagnosis of cutaneous leishmaniasis","volume":"44","author":"Bensoussan","year":"2006"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/88\/3\/article-p583.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/88\/3\/article-p583.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/88\/3\/article-p583.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T18:39:14Z","timestamp":1723055954000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/88\/3\/article-p583.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3,6]]},"references-count":14,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.12-0434","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,6]]}}}