{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:45:12Z","timestamp":1791852312122,"version":"4.3.4"},"reference-count":21,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,5]]},"abstract":"<jats:p>The use of real-time polymerase chain reaction (PCR) for detection of <jats:italic>Strongyloides stercoralis<\/jats:italic> in stool has recently been described. We compared five DNA extraction methods by using normal human stool spiked with <jats:italic>Strongyloides ratti<\/jats:italic> and tested by using a real-time PCR. The PowerSoil kit was found to be the best technique in terms of sensitivity and ease of use. The PCR detected DNA extracted from one spiked <jats:italic>S. ratti<\/jats:italic> larva diluted 10<jats:sup>\u22122<\/jats:sup>. The PowerSoil kit was then used to extract DNA from 160 human survey samples. All culture positive specimens with a high and moderate larval load were identified by real-time PCR, but only 15% of specimens with low larval load were positive. Specificity was greater than 99%. The combination of the PowerSoil kit and real-time PCR reliably detected high to moderate larval numbers of <jats:italic>S. stercoralis<\/jats:italic> in stools but was less sensitive when the larval load was low.<\/jats:p>","DOI":"10.4269\/ajtmh.12-0437","type":"journal-article","created":{"date-parts":[[2013,4,9]],"date-time":"2013-04-09T01:35:47Z","timestamp":1365471347000},"page":"1048-1051","source":"Crossref","is-referenced-by-count":58,"title":["Real-Time Polymerase Chain Reaction for Detection of Strongyloides stercoralis in Stool"],"prefix":"10.4269","volume":"88","author":[{"given":"Yasmin","family":"Sultana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neisha","family":"Jeoffreys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew R.","family":"Watts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwendolyn 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