{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T18:47:14Z","timestamp":1782845234185,"version":"3.54.5"},"reference-count":38,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,5]]},"abstract":"<jats:p>In endemic countries with soil-transmitted helminths mass drug administration with albendazole or mebendazole are being implemented as a control strategy. However, it is well known in veterinary helminths that the use of the same benzimidazole drugs can place selection on the \u03b2-tubulin gene, leading to resistance. Given the concern that resistance could arise in human soil-transmitted helminths, there is an urgent need to develop accurate diagnostic tools for monitoring resistance. In this study, we developed molecular assays to detect putative resistance genetic changes in <jats:italic>Ascaris lumbricoides<\/jats:italic>, <jats:italic>Trichuris trichiura<\/jats:italic>, and hookworms, and we optimized an egg hatch assay for the canine hookworm <jats:italic>Ancylostoma caninum<\/jats:italic> and applied it to <jats:italic>Necator americanus<\/jats:italic>. Both assays were tested on field samples. The molecular assays demonstrated their reproducibility and capacity to detect the presence of worms carrying putative resistance-associated genetic changes. However, further investigations are needed to validate our molecular and biological tests on additional field isolates.<\/jats:p>","DOI":"10.4269\/ajtmh.12-0484","type":"journal-article","created":{"date-parts":[[2013,3,5]],"date-time":"2013-03-05T11:32:46Z","timestamp":1362483166000},"page":"1052-1061","source":"Crossref","is-referenced-by-count":74,"title":["Molecular and Biological Diagnostic Tests for Monitoring Benzimidazole Resistance in Human Soil-Transmitted Helminths"],"prefix":"10.4269","volume":"88","author":[{"given":"A\u00efssatou","family":"Diawara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan M.","family":"Schwenkenbecher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ray M.","family":"Kaplan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger 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