{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T10:36:49Z","timestamp":1785148609215,"version":"3.55.0"},"reference-count":33,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,7,10]]},"abstract":"<jats:p>Molecular methods have been proposed as an alternative tool for the diagnosis of visceral leishmaniasis (VL), but no data are available regarding use for monitoring clinical outcome. A prospective cohort study of human immunodeficiency virus-(HIV) and VL-coinfected patients was conducted in a university-affiliated hospital in Barcelona, Spain. <jats:italic>Leishmania<\/jats:italic> parasite load was monitored using a real-time polymerase chain reaction (PCR) at baseline and every 3 months. Cutoff values for PCR were determined using receiver operating characteristic (ROC) curves. Overall, 37 episodes were analyzed, and 25 of these episodes were considered as relapsing episodes. A significant decrease of parasite load measured 3 months after treatment could predict the clinical evolution of VL. A parasite load over 0.9 parasites\/mL measured 12 months after treatment could predicts relapse with a sensitivity of 100% and a specificity of 90.9%. Monitoring parasite load by an ultrasensitive quantitative <jats:italic>Leishmania<\/jats:italic> PCR is useful to predict the risk of relapse after a VL episode in HIV-infected patients.<\/jats:p>","DOI":"10.4269\/ajtmh.12-0527","type":"journal-article","created":{"date-parts":[[2013,4,30]],"date-time":"2013-04-30T02:53:57Z","timestamp":1367290437000},"page":"105-110","source":"Crossref","is-referenced-by-count":37,"title":["Ultrasensitive Real-Time PCR for the Clinical Management of Visceral Leishmaniasis in HIV-Infected Patients"],"prefix":"10.4269","volume":"89","author":[{"given":"Israel","family":"Molina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roser","family":"Fisa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Riera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicen\u00e7","family":"Falc\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleix","family":"Elizalde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Salvador","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Crespo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Curran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo","family":"L\u00f3pez-Chejade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Tebar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago","family":"P\u00e9rez-Hoyos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban","family":"Ribera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Pahissa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Am 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Chemother","first-page":"837","article-title":"Efficacy of liposomal amphotericin B for secondary prophylaxis of visceral leishmaniasis in HIV-infected patients","volume":"60","author":"Molina","year":"2007"},{"key":"R6","series-title":"Am J Trop Med Hyg","first-page":"276","article-title":"The value of a new microculture method for diagnosis of visceral leishmaniasis by using bone marrow and peripheral blood","volume":"73","author":"Allahverdiyev","year":"2005"},{"key":"R7","series-title":"J Clin Microbiol","first-page":"1145","article-title":"PCR and in vitro cultivation for detection of Leishmania spp. in diagnostic samples from human and dogs","volume":"33","author":"Mathis","year":"1995"},{"key":"R8","series-title":"AIDS","first-page":"452","article-title":"Interest and reliability of a polymerase chain reaction on bone marrow samples in the diagnosis of visceral leishmaniasis in AIDS","volume":"10","author":"Piarroux","year":"1996"},{"key":"R9","series-title":"J Clin Microbiol","first-page":"1831","article-title":"PCR enzyme-linked immunosorbent assay for diagnosis of leishmaniasis in human immunodeficiency virus\u2013infected patients","volume":"34","author":"Costa","year":"1996"},{"key":"R10","series-title":"Eur J Clin Microbiol Infect Dis","first-page":"396","article-title":"Detection of Leishmania in immunocompromised patients using peripheral blood spots on filter paper and the polymerase chain reaction","volume":"19","author":"Campino","year":"2000"},{"key":"R11","series-title":"J Clin Microbiol","first-page":"357","article-title":"Role of PCR in diagnosis and prognosis of visceral leishmaniasis in patients coinfected with human immunodeficiency virus type 1","volume":"39","author":"Pizzuto","year":"2001"},{"issue":"(Suppl 1)","key":"R12","series-title":"Trans R Soc Trop Med Hyg","first-page":"S191","article-title":"A nested polymerase chain reaction for diagnosis and follow-up of human visceral leishmaniasis patients using blood samples","volume":"96","author":"Fisa","year":"2002"},{"issue":"(Suppl 1)","key":"R13","series-title":"Trans R Soc Trop Med Hyg","first-page":"S185","article-title":"A nested polymerase chain reaction (Ln-PCR) for diagnosing and monitoring Leishmania infantum in patients coinfected with human immunodeficiency virus","volume":"96","author":"Cruz","year":"2002"},{"key":"R14","series-title":"Ann Trop Med Parasitol","first-page":"669","article-title":"The high sensitivity of a PCR-ELISA in the diagnosis of cutaneous and visceral leishmaniasis caused by Leishmania infantum","volume":"96","author":"Martin-Sanchez","year":"2002"},{"issue":"(Suppl 1)","key":"R15","series-title":"Ann Trop Med Parasitol","first-page":"S115","article-title":"The biological diagnosis of leishmaniasis in HIV-infected patients","volume":"97","author":"Deniau","year":"2003"},{"key":"R16","series-title":"J Clin Microbiol","first-page":"5080","article-title":"Real-time PCR assay for clinical management of human immunodeficiency virus\u2013infected patients with visceral leishmaniasis","volume":"41","author":"Bossolasco","year":"2003"},{"key":"R17","series-title":"J Clin Microbiol","first-page":"5332","article-title":"Convenience of serum for visceral leishmaniasis diagnosis by PCR","volume":"42","author":"Fissore","year":"2004"},{"key":"R18","series-title":"Ann Trop Med Parasitol","first-page":"667","article-title":"Diagnosis of visceral leishmaniasis: the sensitivities and specificities of traditional methods and a nested PCR assay","volume":"98","author":"Gatti","year":"2004"},{"key":"R19","series-title":"J Clin Microbiol","first-page":"236","article-title":"Optimized PCR using patient blood samples for diagnosis and follow-up of visceral Leishmaniasis, with special reference to AIDS patients","volume":"38","author":"Lachaud","year":"2000"},{"key":"R20","series-title":"Am J Trop Med Hyg","first-page":"46","article-title":"Is real-time polymerase chain reaction (PCR) more useful than a conventional PCR for the clinical management of leishmaniasis?","volume":"81","author":"Antinori","year":"2009"},{"key":"R21","series-title":"J Clin Microbiol","first-page":"5249","article-title":"Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity","volume":"42","author":"Mary","year":"2004"},{"key":"R22","series-title":"Am J Trop Med Hyg","first-page":"1012","article-title":"Value of culture and nested PCR of blood in the prediction of relapses in patients co-infected with Leishmania and HIV","volume":"73","author":"Riera","year":"2005"},{"key":"R23","series-title":"Am J Trop Med Hyg","first-page":"858","article-title":"Reference values for Leishmania infantum parasitemia in different clinical presentations: quantitative polymerase chain reaction for therapeutic monitoring and patient follow-up","volume":"75","author":"Mary","year":"2006"},{"key":"R24","series-title":"Clin Infect Dis","first-page":"1602","article-title":"Clinical use of polymerase 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