{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T14:42:24Z","timestamp":1771166544885,"version":"3.50.1"},"reference-count":17,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,5]]},"abstract":"<jats:p>Recent studies using an internal transcribed spacer (ITS)-based real-time polymerase chain reaction (PCR) for the detection of <jats:italic>Schistosoma<\/jats:italic> DNA in urine samples has shown high sensitivity and specificity when performed on controls and known microscopy-positive samples. In this study, using 730 urine samples collected from children in five primary schools from different communities in the Greater Accra region of Ghana, specific detection of <jats:italic>Schistosoma<\/jats:italic> DNA showed excellent sensitivity of 100% and 85.2% in urines with &gt; 50 eggs\/10 mL urine and \u2264 50 eggs\/10 mL of urine, respectively. Additionally, <jats:italic>Schistosoma<\/jats:italic>-specific DNA was amplified in 102 of 673 samples in which <jats:italic>Schistosoma<\/jats:italic> eggs could not be detected with microscopy. Taking microscopy and\/or PCR-positive samples as true positives, the negative predictive value calculated was 94.6\u2013100% for each school sampled as compared with 54.3\u201395.7% using microscopy. This ITS-based real-time PCR proves to be a powerful tool in epidemiological surveys of schistosomiasis providing more precise and sensitive results than microscopy.<\/jats:p>","DOI":"10.4269\/ajtmh.12-0571","type":"journal-article","created":{"date-parts":[[2013,3,26]],"date-time":"2013-03-26T01:38:40Z","timestamp":1364261920000},"page":"1028-1031","source":"Crossref","is-referenced-by-count":49,"title":["Molecular Diagnosis of Schistosoma Infections in Urine Samples of School Children in Ghana"],"prefix":"10.4269","volume":"88","author":[{"given":"Yvonne A.","family":"Aryeetey","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Essien-Baidoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene 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