{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T08:08:19Z","timestamp":1771661299255,"version":"3.50.1"},"reference-count":33,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,3,6]]},"abstract":"<jats:p>The analysis of promastigote excreted\u2013secreted antigen (ESA) reactivity with 53 visceral leishmaniasis (VL) cases showed that each sample reacted regardless of the antigen or the <jats:italic>Leishmania<\/jats:italic> species used in enzyme-linked immunosorbent assay (ELISA) displayed 100% positivity with the <jats:italic>L.<\/jats:italic> (<jats:italic>L.<\/jats:italic>) <jats:italic>chagasi<\/jats:italic> ESA-blot recognizing bands of molecular weight ranging from 26.5 to 31.5 kDa. The analysis of 160 non-visceral cases showed that 5% of the samples cross-reacted with the <jats:italic>L.<\/jats:italic> (<jats:italic>L.<\/jats:italic>) <jats:italic>chagasi<\/jats:italic> ESA-ELISA and 9.4% reacted with the ESA isolated from <jats:italic>L.<\/jats:italic> (<jats:italic>L.<\/jats:italic>) <jats:italic>amazonensis<\/jats:italic> and <jats:italic>L.<\/jats:italic> (<jats:italic>V.<\/jats:italic>) <jats:italic>braziliensis<\/jats:italic>, whereas a high cross-reaction ranging from 24.4% to 25% was observed with total crude promastigote antigens (PRO-ELISA). The ESA-blot of <jats:italic>L.<\/jats:italic> (<jats:italic>L.<\/jats:italic>) <jats:italic>chagasi<\/jats:italic> tested with non-visceral sera samples showed a cross-reaction with 8.8% of cases; most of these cases represented tegumentary leishmaniasis and only one acute chagasic case. These data lead us to recommend the use of ESA as an alternative antigen in VL diagnosis.<\/jats:p>","DOI":"10.4269\/ajtmh.12-0587","type":"journal-article","created":{"date-parts":[[2013,1,17]],"date-time":"2013-01-17T05:43:24Z","timestamp":1358401404000},"page":"559-565","source":"Crossref","is-referenced-by-count":10,"title":["The Efficacy of L. 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