{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T12:55:13Z","timestamp":1791809713823,"version":"4.3.4"},"reference-count":14,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,8,7]]},"abstract":"\n One hundred and eighty febrile patients were analyzed in a prospective evaluation of\n Orientia tsutsugamushi<\/jats:italic>\n and\n Rickettsia<\/jats:italic>\n spp. real-time polymerase chain reaction (PCR) assays for early diagnosis of rickettsial infections. By paired serology, 3.9% (7 of 180) and 6.1% (11 of 180) of patients were confirmed to have acute scrub or murine typhus, respectively. The PCR assays for the detection of\n O. tsutsugamushi<\/jats:italic>\n and\n Rickettsia<\/jats:italic>\n spp. had high specificity (99.4% [95% confidence interval (CI): 96.8\u2013100] and 100% [95% CI: 97.8\u2013100], respectively). The PCR results were also compared with immunoglobulin M (IgM) immunofluorescence assay (IFA) on acute sera. For\n O. tsutsugamushi<\/jats:italic>\n , PCR sensitivity was twice that of acute specimen IgM IFA (28.6% versus 14.3%; McNemar's\n P<\/jats:italic>\n = 0.3). For\n Rickettsia<\/jats:italic>\n spp., PCR was four times as sensitive as acute specimen IgM IFA (36.4% versus 9.1%;\n P<\/jats:italic>\n = 0.08), although this was not statistically significant. Whole blood and buffy coat, but not serum, were acceptable specimens for these PCRs. Further evaluation of these assays in a larger prospective study is warranted.\n <\/jats:p>","DOI":"10.4269\/ajtmh.12-0600","type":"journal-article","created":{"date-parts":[[2013,6,4]],"date-time":"2013-06-04T01:42:47Z","timestamp":1370310167000},"page":"308-310","source":"Crossref","is-referenced-by-count":45,"title":["A Prospective Evaluation of Real-Time PCR Assays for the Detection of Orientia tsutsugamushi and Rickettsia spp. for Early Diagnosis of Rickettsial Infections during the Acute Phase of Undifferentiated Febrile Illness"],"prefix":"10.4269","volume":"89","author":[{"given":"Wanitda","family":"Watthanaworawit","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Turner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Turner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ampai","family":"Tanganuchitcharnchai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allen L.","family":"Richards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin M.","family":"Bourzac","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart D.","family":"Blacksell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Nosten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Ann Trop Med Parasitol","first-page":"363","article-title":"Causes of acute, undifferentiated, febrile illness in rural Thailand: results of a prospective observational study","volume":"100","author":"Suttinont","year":"2006"},{"key":"R2","series-title":"PLoS Negl Trop Dis","first-page":"e888","article-title":"Arthropod borne disease: the leading cause of fever in pregnancy on the Thai-Burmese border","volume":"4","author":"McGready","year":"2010"},{"key":"R3","series-title":"Am J Trop Med Hyg","first-page":"108","article-title":"Causes of fever in adults on the Thai-Myanmar border","volume":"74","author":"Ellis","year":"2006"},{"key":"R4","series-title":"Southeast Asian J Trop 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Hyg","first-page":"497","article-title":"Comparative evaluation of selected diagnostic assays for the detection of IgG and IgM antibody to Orientia tsutsugamushi in Thailand","volume":"67","author":"Coleman","year":"2002"},{"key":"R13","series-title":"PLoS Negl Trop Dis","first-page":"e1307","article-title":"Diagnostic accuracy of a loop-mediated isothermal PCR assay for detection of Orientia tsutsugamushi during acute scrub typhus infection","volume":"5","author":"Paris","year":"2011"},{"key":"R14","series-title":"Am J Trop Med Hyg","first-page":"368","article-title":"Diagnosis of scrub typhus","volume":"82","author":"Koh","year":"2010"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/89\/2\/article-p308.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/89\/2\/article-p308.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/89\/2\/article-p308.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T14:27:11Z","timestamp":1723040831000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/89\/2\/article-p308.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,7]]},"references-count":14,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.12-0600","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,7]]}}}