{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T18:52:54Z","timestamp":1793127174421,"version":"4.5.0"},"reference-count":15,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,2,5]]},"abstract":"<jats:p><jats:italic>Sarcocystis nesbitti<\/jats:italic> is an intracellular protozoan parasite found as sarcocysts within muscle fibers of intermediate hosts (monkey and baboon). The definitive host is suspected to be the snake. We report two cases from a larger cohort of 89 patients who had fever, headache, and generalized myalgia after a trip to Pangkor Island, Malaysia. Sarcocysts were detected in skeletal muscle biopsy specimens by light and electron microscopy from these two patients. DNA sequencing based on the 18S ribosomal DNA region identified the <jats:italic>Sarcocystis<\/jats:italic> species as <jats:italic>S. nesbitti<\/jats:italic>. We also identified <jats:italic>S. nesbitti<\/jats:italic> sequences in the stools of a snake (<jats:italic>Naja naja<\/jats:italic>). Phylogenetic analysis showed that these sequences form a cluster with most of the other known <jats:italic>Sarcocystis<\/jats:italic> species for which the snake is a definitive host. We believe these two patients were likely to have symptomatic acute muscular sarcocystosis after <jats:italic>S. nesbitti<\/jats:italic> infection that may have originated from snakes.<\/jats:p>","DOI":"10.4269\/ajtmh.12-0678","type":"journal-article","created":{"date-parts":[[2014,1,14]],"date-time":"2014-01-14T05:33:27Z","timestamp":1389677607000},"page":"361-364","source":"Crossref","is-referenced-by-count":60,"title":["Sarcocystis nesbitti Infection in Human Skeletal Muscle: Possible Transmission from Snakes"],"prefix":"10.4269","volume":"90","author":[{"given":"Yee Ling","family":"Lau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phooi Yee","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong Tin","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mun Yik","family":"Fong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohela","family":"Mahmud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kum Thong","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Clin Microbiol Rev","first-page":"894","article-title":"Sarcocystis spp. in human infections","volume":"17","author":"Fayer","year":"2004"},{"key":"R2","series-title":"Am J Trop Med Hyg","first-page":"634","article-title":"Light and electron microstructure of a Sarcocystis sp. from the Malaysian long-tailed monkey, Macaca fascicularis","volume":"28","author":"Kan","year":"1979"},{"key":"R3","series-title":"Parasitol Res","first-page":"138","article-title":"Light microscopic and three-dimensional morphology of the human muscular 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Prevention","year":"2012"},{"key":"R13","series-title":"Mol Biol Evol","first-page":"1596","article-title":"MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0","volume":"24","author":"Tamura","year":"2007"},{"key":"R14","series-title":"Southeast Asian J Public Health Trop Med","first-page":"247","article-title":"Human Sarcocystis infection in Malaysia","volume":"12","author":"Pathmanathan","year":"1981"},{"key":"R15","series-title":"Am J Trop Med Hyg","first-page":"819","article-title":"Sarcocystis in man: a review and report of five cases","volume":"28","author":"Beaver","year":"1979"}],"container-title":["The American Society of Tropical Medicine and 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