{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T14:43:04Z","timestamp":1781016184835,"version":"3.54.1"},"reference-count":44,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,9,4]]},"abstract":"<jats:p>Rickettsioses caused by <jats:italic>Rickettsia felis<\/jats:italic> are an emergent global threat. Historically, the northern region of the province of Caldas in Colombia has reported murine typhus cases, and recently, serological studies confirmed high seroprevalence for both <jats:italic>R. felis<\/jats:italic> and <jats:italic>R. typhi<\/jats:italic>. In the present study, fleas from seven municipalities were collected from dogs, cats, and mice. DNA was extracted and amplified by polymerase chain reaction (PCR) to identify <jats:italic>gltA<\/jats:italic>, <jats:italic>ompB<\/jats:italic>, and <jats:italic>17kD<\/jats:italic> genes. Positive samples were sequenced to identify the species of <jats:italic>Rickettsia<\/jats:italic>. Of 1,341 fleas, <jats:italic>Ctenocephalides felis<\/jats:italic> was the most prevalent (76.7%). Positive PCR results in the three genes were evidenced in <jats:italic>C. felis<\/jats:italic> (minimum infection rates; 5.3%), <jats:italic>C. canis<\/jats:italic> (9.2%), and <jats:italic>Pulex irritans<\/jats:italic> (10.0%). Basic Local Alignment Search Tool (BLAST) analyses of sequences showed high identity values (&gt; 98%) with <jats:italic>R. felis<\/jats:italic>, and all were highly related by phylogenetic analyses. This work shows the first detection of <jats:italic>R. felis<\/jats:italic> in fleas collected from animals in Colombia.<\/jats:p>","DOI":"10.4269\/ajtmh.12-0698","type":"journal-article","created":{"date-parts":[[2013,7,23]],"date-time":"2013-07-23T04:16:59Z","timestamp":1374553019000},"page":"453-459","source":"Crossref","is-referenced-by-count":22,"title":["Molecular Detection of Rickettsia felis in Different Flea Species from Caldas, Colombia"],"prefix":"10.4269","volume":"89","author":[{"given":"Alejandro","family":"Ram\u00edrez-Hern\u00e1ndez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"Montoya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge E.","family":"P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Mercado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"de la Ossa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"V\u00e9lez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria","family":"Estrada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria I.","family":"Correa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Duque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan S.","family":"Ariza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesar","family":"Henao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Valbuena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marylin","family":"Hidalgo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Clin Microbiol Rev","first-page":"694","article-title":"Rickettsioses as paradigms of new or emerging infectious diseases","volume":"10","author":"Raoult","year":"1997"},{"key":"R2","series-title":"Emerg Infect Dis","first-page":"1019","article-title":"Rickettsia felis as emergent global threat for humans","volume":"14","author":"Perez-Osorio","year":"2008"},{"key":"R3","series-title":"Emerg Health Threats 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