{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:05:42Z","timestamp":1790741142715,"version":"4.1.0"},"reference-count":26,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,5]]},"abstract":"<jats:p>Diagnosis of gastrointestinal parasites has traditionally relied on stool microscopy, which has low diagnostic sensitivity and specificity. We have developed a novel, rapid, high-throughput quantitative multi-parallel real-time polymerase chain reaction (qPCR) platform. Species-specific primers\/probes were used for eight common gastrointestinal parasite pathogens: <jats:italic>Ascaris lumbricoides<\/jats:italic>, <jats:italic>Necator americanus<\/jats:italic>, <jats:italic>Ancylostoma duodenale<\/jats:italic>, <jats:italic>Giardia lamblia<\/jats:italic>, <jats:italic>Cryptosporidium<\/jats:italic> spp., <jats:italic>Entamoeba histolytica<\/jats:italic>, <jats:italic>Trichuris trichiura<\/jats:italic>, and <jats:italic>Strongyloides stercoralis<\/jats:italic>. Stool samples from 400 13-month-old children in rural Ecuador were analyzed and the qPCR was compared with a standard direct wet mount slide for stool microscopy, as were 125 8\u201314-year-old children before and after anthelmintic treatment. The qPCR showed higher detection rates for all parasites compared with direct microscopy, <jats:italic>Ascaris<\/jats:italic> (7.0% versus 5.5%) and for <jats:italic>Giardia<\/jats:italic> (31.5% versus 5.8%). Using an enhanced DNA extraction method, we were able to detect <jats:italic>T. trichiura<\/jats:italic> DNA. These assays will be useful to refine treatment options for affected populations, ultimately leading to better health outcomes.<\/jats:p>","DOI":"10.4269\/ajtmh.12-0726","type":"journal-article","created":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T07:15:38Z","timestamp":1363677338000},"page":"1041-1047","source":"Crossref","is-referenced-by-count":239,"title":["A Novel, Multi-Parallel, Real-Time Polymerase Chain Reaction Approach for Eight Gastrointestinal Parasites Provides Improved Diagnostic Capabilities to Resource-Limited At-Risk Populations"],"prefix":"10.4269","volume":"88","author":[{"given":"Rojelio","family":"Mejia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosselin","family":"Vicu\u00f1a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nely","family":"Broncano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Sandoval","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maritza","family":"Vaca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha","family":"Chico","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip J.","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas 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