{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T01:45:11Z","timestamp":1770342311846,"version":"3.49.0"},"reference-count":26,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,5,1]]},"abstract":"<jats:p>Toxoplasmosis in humans and other animals is caused by the protozoan parasite <jats:italic>Toxoplasma gondii<\/jats:italic>. During the process of host cell invasion and parasitophorous vacuole formation by the tachyzoites, the parasite secretes Rhoptry protein 8 (<jats:italic>ROP8<\/jats:italic>), an apical secretory organelle. Thus, <jats:italic>ROP8<\/jats:italic> is an important protein for the pathogenesis of <jats:italic>T. gondii<\/jats:italic>. The <jats:italic>ROP8<\/jats:italic> DNA was constructed into a pVAX-1 vaccine vector and used for immunizing BALB\/c mice. Immunized mice developed immune response characterized by significant antibody responses, antigen-specific proliferation of spleen cells, and production of high levels of IFN-\u03b3 (816 \u00b1 26.3 pg\/mL). Challenge experiments showed significant levels of increase in the survival period (29 days compared with 9 days in control) in <jats:italic>ROP8<\/jats:italic> DNA vaccinated mice after a lethal challenge with <jats:italic>T. gondii<\/jats:italic>. Results presented in this study suggest that <jats:italic>ROP8<\/jats:italic> DNA is a promising and potential vaccine candidate against toxoplasmosis.<\/jats:p>","DOI":"10.4269\/ajtmh.12-0727","type":"journal-article","created":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T07:15:38Z","timestamp":1363677338000},"page":"883-887","source":"Crossref","is-referenced-by-count":34,"title":["Protective Immune Response in BALB\/c Mice Induced by DNA Vaccine of the ROP8 gene of Toxoplasma gondii"],"prefix":"10.4269","volume":"88","author":[{"given":"Sonaimuthu","family":"Parthasarathy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mun Yik","family":"Fong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kalyanasundaram","family":"Ramaswamy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yee 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