{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:40:31Z","timestamp":1790019631912,"version":"4.0.1"},"reference-count":10,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,8,7]]},"abstract":"<jats:p>Leptospirosis may be caused by &gt; 250 <jats:italic>Leptospira<\/jats:italic> serovars. Serovar classification is a complex task that most laboratories cannot perform. We assessed the interlaboratory reproducibility of a pulsed-field gel electrophoresis (PFGE) identification technique developed by the Centers for Disease Control and Prevention (CDC). Blinded exchange of 93 Leptospiraceae strains occurred between San Antonio Military Medical Center (SAMMC) and the CDC. PFGE was performed and gel images were analyzed and compared with patterns present in each laboratory's database (CDC database: &gt; 800 strain patterns; SAMMC database: &gt; 300 strain patterns). Overall, 93.7% (74 of 79) of strains present in each receiving laboratory's database were correctly identified. Five isolates were misidentified, and two isolates did not match serovar PFGE patterns in the receiving laboratory's database. Patterns for these seven isolates were identical between laboratories; four serovars represented misidentified reference strains. The PFGE methodology studied showed excellent interlaboratory reproducibility, enabling standardization and data sharing between laboratories.<\/jats:p>","DOI":"10.4269\/ajtmh.12-0768","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T01:52:53Z","timestamp":1372729973000},"page":"380-384","source":"Crossref","is-referenced-by-count":6,"title":["Interlaboratory Agreement of Pulsed-Field Gel Electrophoresis Identification of Leptospira Serovars"],"prefix":"10.4269","volume":"89","author":[{"given":"Katrin","family":"Mende","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renee L.","family":"Galloway","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara J.","family":"Becker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam L.","family":"Beckius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clinton K.","family":"Murray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duane R.","family":"Hospenthal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Rev Soc Bras Med Trop","first-page":"166","article-title":"Characterization of Leptospira spp. reference strains using the pulsed-field gel electrophoresis technique","volume":"43","author":"Machry","year":"2010"},{"key":"R2","series-title":"Am J Trop Med Hyg","first-page":"628","article-title":"Evaluation of a modified pulsed-field gel electrophoresis approach for the identification of Leptospira serovars","volume":"78","author":"Galloway","year":"2008"},{"key":"R3","series-title":"PLoS Negl Trop Dis","first-page":"e824","article-title":"Application and validation of PFGE for serovar identification of Leptospira clinical isolates","volume":"4","author":"Galloway","year":"2010"},{"key":"R4","series-title":"J Clin Microbiol","first-page":"1696","article-title":"Pulsed-field gel electrophoresis of NotI digests of leptospiral DNA: a new rapid method of serovar identification","volume":"30","author":"Herrmann","year":"1992"},{"key":"R5","series-title":"Lett Appl Microbiol","first-page":"623","article-title":"Application of pulsed-field gel electrophoresis for the discrimination of leptospiral isolates in Brazil","volume":"48","author":"Romero","year":"2009"},{"key":"R6","series-title":"FEMS Microbiol Lett","first-page":"174","article-title":"Molecular analysis of Leptospira spp. isolated from humans by restriction fragment length polymorphism, real-time PCR and pulsed-field gel electrophoresis","volume":"300","author":"Turk","year":"2009"},{"key":"R7","series-title":"Am J Trop Med Hyg","first-page":"889","article-title":"Serologic and molecular studies of Leptospira and leptospirosis among rats in the Philippines","volume":"82","author":"Villanueva","year":"2010"},{"key":"R8","series-title":"Am J Trop Med Hyg","first-page":"83","article-title":"Monitoring Leptospira strain collections: the need for quality control","volume":"82","author":"Cerqueira","year":"2010"},{"key":"R9","series-title":"J Clin Microbiol","first-page":"581","article-title":"Multilocus sequence analysis for typing Leptospira interrogans and Leptospira kirschneri","volume":"48","author":"Leon","year":"2010"},{"key":"R10","series-title":"Southeast Asian J Trop Med Public Health","first-page":"97","article-title":"Identification of clinical isolates of Leptospira spp. by pulsed-field gel electrophoresis and microscopic agglutination test","volume":"38","author":"Naigowit","year":"2007"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/89\/2\/article-p380.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/89\/2\/article-p380.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/89\/2\/article-p380.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T18:26:47Z","timestamp":1723055207000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/89\/2\/article-p380.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,7]]},"references-count":10,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.12-0768","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,7]]}}}