{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:23:36Z","timestamp":1792661016959,"version":"4.5.0"},"reference-count":60,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,12,4]]},"abstract":"<jats:p>We produced three highly purified recombinant antigens rLipL32, rLipL41, and rLigA-Rep (leptospiral immunoglobulin-like A repeat region) for the detection of <jats:italic>Leptospira<\/jats:italic>-specific antibodies in an enzyme-linked immunosorbent assay (ELISA). The performance of these recombinant antigens was evaluated using 121 human sera. Among them, 63 sera were microscopic agglutination test (MAT)-confirmed positive sera from febrile patients in Peru, 22 sera were indigenous MAT-negative febrile patient sera, and 36 sera were from patients with other febrile diseases from Southeast Asia, where leptospirosis is also endemic. Combining the results of immunoglobulin M (IgM) and IgG detection from these three antigens, the overall sensitivity is close to 90% based on the MAT. These results suggest that an ELISA using multiple recombinant antigens may be used as an alternative method for the detection of <jats:italic>Leptospira<\/jats:italic>-specific antibodies.<\/jats:p>","DOI":"10.4269\/ajtmh.13-0041","type":"journal-article","created":{"date-parts":[[2013,10,29]],"date-time":"2013-10-29T00:15:45Z","timestamp":1383005745000},"page":"1088-1094","source":"Crossref","is-referenced-by-count":18,"title":["Detection of Leptospira-Specific Antibodies Using a Recombinant Antigen-Based Enzyme-Linked Immunosorbent Assay"],"prefix":"10.4269","volume":"89","author":[{"given":"Hua-Wei","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric 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nations","volume":"19","author":"Cruz","year":"2009"},{"key":"R5","series-title":"PLoS Negl Trop Dis","first-page":"579","article-title":"Increasing trends of leptospirosis in northern India: a clinico-epidemiological study","volume":"4","author":"Sethi","year":"2010"},{"key":"R6","series-title":"BMC Infect Dis","first-page":"147","article-title":"Leptospirosis in the Asia Pacific region","volume":"9","author":"Victoriano","year":"2009"},{"key":"R7","series-title":"Malays J Pathol","first-page":"1","article-title":"Leptospirosis: a re-emerging infection","volume":"33","author":"Lim","year":"2011"},{"key":"R8","series-title":"Emerg Infect Dis","first-page":"1678","article-title":"Leptospirosis as frequent cause of acute febrile illness in southern Sri Lanka","volume":"17","author":"Reller","year":"2011"},{"key":"R9","series-title":"J Clin Microbiol","first-page":"307","article-title":"Human leptospira isolates circulating in Mayotte (Indian Ocean) have unique serological and molecular features","volume":"50","author":"Bourhy","year":"2012"},{"key":"R10","series-title":"Rev Soc Bras Med Trop","first-page":"100","article-title":"Geographic analysis on the occurrence of human and canine leptospirosis in the city of Maring\u00e1, state of Paran\u00e1, Brazil","volume":"45","author":"Fonzar","year":"2012"},{"key":"R11","series-title":"Am J Trop Med Hyg","first-page":"309","article-title":"Leptospirosis in American Samoa 2010: epidemiology, environmental drivers, and the management of emergence","volume":"86","author":"Lau","year":"2012"},{"key":"R12","series-title":"Microbes Infect","first-page":"317","article-title":"Leptospirosis prevalence in Chinese populations in the last two decades","volume":"14","author":"Zhang","year":"2012"},{"key":"R13","series-title":"Ann Intern Med","first-page":"794","article-title":"Sporadic urban leptospirosis","volume":"125","author":"Vinetz","year":"1996"},{"key":"R14","series-title":"Emerg Infect Dis","first-page":"702","article-title":"Leptospirosis in \u201cEco-Challenge\u201d atheletes, Malaysian Borneo, 2000","volume":"9","author":"Sejvar","year":"2003"},{"key":"R15","series-title":"Clin Infect Dis","first-page":"1593","article-title":"Outbreak of leptospirosis among triathlon participants and community residents in Springfield, Illinois, 1998","volume":"34","author":"Morgan","year":"2002"},{"key":"R16","series-title":"MMWR Morb Mortal Wkly Rep","first-page":"577","article-title":"Outbreak of leptospirosis among white-water rafters\u2014Costa Rica, 1996","volume":"46","author":"Centers for Disease Control and Prevention","year":"1997"},{"key":"R17","series-title":"Epidemiol Infect","first-page":"195","article-title":"A cluster of leptospirosis cases in canoeists following a competition on the River Liffey","volume":"132","author":"Boland","year":"2004"},{"key":"R18","series-title":"Wien Klin Wochenschr","first-page":"751","article-title":"Outbreak of leptospirosis among triathlon participants in Langau, Austria, 2010","volume":"123","author":"Radl","year":"2011"},{"key":"R19","series-title":"Expert Rev Anti Infect Ther","first-page":"95","article-title":"Infectious diseases following natural disasters: prevention and control measures","volume":"10","author":"Kouadio","year":"2012"},{"key":"R20","series-title":"Emerg Infect Dis","first-page":"91","article-title":"Outbreak of leptospirosis after flood, the Philippines, 2009","volume":"18","author":"Amilasan","year":"2012"},{"key":"R21","series-title":"Am J Trop Med Hyg","first-page":"187","article-title":"Leptospirosis after typhoon","volume":"86","author":"Lin","year":"2012"},{"key":"R22","series-title":"Epidemiol Infect","first-page":"1","article-title":"Leptospirosis following a major flood in Central Queensland, Australia","volume":"25","author":"Smith","year":"2012"},{"key":"R23","series-title":"Leptospira and Leptospirosis","author":"Faine","year":"1999"},{"key":"R24","series-title":"Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control","author":"WHO","year":"2003"},{"key":"R25","series-title":"Manual of Clinical Microbiology","first-page":"963","article-title":"Leptospira","author":"Levett","year":"2007"},{"key":"R26","series-title":"Guidelines for the Control of Leptospirosis","author":"Faine","year":"1982"},{"key":"R27","series-title":"Am J Trop Med Hyg","first-page":"731","article-title":"Assessment of the efficacy of the IgM enzyme-linked immunosorbent assay (ELISA) and microscopic agglutination test (MAT) in the diagnosis of acute leptospirosis","volume":"61","author":"Cumberland","year":"1999"},{"key":"R28","series-title":"Clin Diagn Lab Immunol","first-page":"166","article-title":"Lateral-flow assay for rapid serodiagnosis of human leptospirosis","volume":"8","author":"Smits","year":"2001"},{"key":"R29","series-title":"J Clin Microbiol","first-page":"1464","article-title":"Evaluation of eight rapid screening tests for acute leptospirosis in Hawaii","volume":"40","author":"Effler","year":"2002"},{"key":"R30","series-title":"Am J Trop Med Hyg","first-page":"745","article-title":"Evaluation of two enzyme-linked immunosorbent assay methods for detection of immunoglobulin M antibodies in acute leptospirosis","volume":"66","author":"Levett","year":"2002"},{"key":"R31","series-title":"Epidemiol Infect","first-page":"617","article-title":"Evaluation of Lepto Dri Dot as a rapid test for the diagnosis of leptospirosis","volume":"129","author":"Vijayachari","year":"2002"},{"key":"R32","series-title":"J Clin Microbiol","first-page":"803","article-title":"Evaluation of four commercially available rapid serologic tests for diagnosis of leptospirosis","volume":"41","author":"Bajani","year":"2003"},{"key":"R33","series-title":"J Med Microbiol","first-page":"897","article-title":"Field application of Lepto lateral flow for rapid diagnosis of leptospirosis","volume":"52","author":"Sehgal","year":"2003"},{"key":"R34","series-title":"Curr Opin Infect Dis","first-page":"376","article-title":"Leptospirosis","volume":"18","author":"McBride","year":"2005"},{"key":"R35","series-title":"Clin Vaccine Immunol","first-page":"1166","article-title":"Limited diagnostic capacity of two commercial assays for the detection of leptospira immunoglobulin M antibodies in Laos","volume":"3","author":"Blacksell","year":"2006"},{"key":"R36","series-title":"Am J Trop Med Hyg","first-page":"524","article-title":"Accuracy of a commercial IgM ELISA for the diagnosis of human leptospirosis in Thailand","volume":"86","author":"Desakorn","year":"2012"},{"key":"R37","series-title":"Nature","first-page":"888","article-title":"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing","volume":"422","author":"Ren","year":"2003"},{"key":"R38","series-title":"J Bacteriol","first-page":"2164","article-title":"Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis","volume":"186","author":"Nascimento","year":"2004"},{"key":"R39","series-title":"Proc Natl Acad Sci USA","first-page":"14560","article-title":"Genome reduction in Leptospira borgpetersenii reflects limited transmission potential","volume":"103","author":"Bulach","year":"2006"},{"key":"R40","series-title":"PLoS One","first-page":"e1607","article-title":"Genome sequence of the saprophyte leptospira biflexa provided insights into the evolution of Leptospira and the pathogenesis of leptospirosis","volume":"3","author":"Picardeau","year":"2008"},{"key":"R41","series-title":"Lancet Infect Dis","first-page":"757","article-title":"Leptospirosis: a zoonotic disease of global importance","volume":"3","author":"Bharti","year":"2003"},{"key":"R42","series-title":"Infect Immun","first-page":"2276","article-title":"The leptospiral major outer membrane protein LipL32 is a lipoprotein expressed during mammalian infection","volume":"68","author":"Haake","year":"2000"},{"key":"R43","series-title":"Infect Immun","first-page":"2322","article-title":"Molecular cloning and sequence analysis of the gene coding LipL41, a surface-exposed lipoprotein of pathogenic Leptospira species","volume":"64","author":"Shang","year":"1996"},{"key":"R44","series-title":"Mol Microbiol","first-page":"929","article-title":"Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily","volume":"49","author":"Matsunaga","year":"2003"},{"key":"R45","series-title":"Infect Immun","first-page":"4853","article-title":"Surfaceome of Leptospira spp","volume":"73","author":"Cullen","year":"2005"},{"key":"R46","series-title":"Infect Genet Evol","first-page":"196","article-title":"Genetic diversity of the leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp","volume":"9","author":"Mcbride","year":"2009"},{"key":"R47","series-title":"Infect 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