{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:18:21Z","timestamp":1788931101279,"version":"build-2803163510"},"reference-count":14,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,7,10]]},"abstract":"<jats:p>The diagnosis of melioidosis depends on the culture of <jats:italic>Burkholderia pseudomallei,<\/jats:italic> which takes at least 48 hours. We used a polyclonal-FITC-based immunofluorescence microscopic assay (Pab-IFA) on clinical samples to provide a rapid presumptive diagnosis. This has limitations including photobleaching and batch-to-batch variability. This study evaluated an IFA based on a monoclonal antibody specific to <jats:italic>B. pseudomallei<\/jats:italic> (Mab-IFA) and Alexa Fluor 488. A diagnostic evaluation was performed on a prospective cohort of 951 consecutive patients with suspected melioidosis. A total of 1,407 samples were tested. Test accuracy was defined against culture as the gold standard, and was also compared against Pab-IFA. A total of 88 samples from 64 patients were culture positive for <jats:italic>B. pseudomallei<\/jats:italic>. The diagnostic sensitivity and specificity of the Mab-IFA was comparable to the Pab-IFA (48.4% versus 45.3% for sensitivity, and 99.8% versus 98.8% for specificity). We have incorporated the Mab-IFA into our routine practice.<\/jats:p>","DOI":"10.4269\/ajtmh.13-0066","type":"journal-article","created":{"date-parts":[[2013,5,29]],"date-time":"2013-05-29T06:38:16Z","timestamp":1369809496000},"page":"165-168","source":"Crossref","is-referenced-by-count":35,"title":["Monoclonal Antibody-Based Immunofluorescence Microscopy for the Rapid Identification of Burkholderia pseudomallei in Clinical Specimens"],"prefix":"10.4269","volume":"89","author":[{"given":"Sarunporn","family":"Tandhavanant","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gumphol","family":"Wongsuvan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanaporn","family":"Wuthiekanun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nittaya","family":"Teerawattanasook","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas P. 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