{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T02:55:12Z","timestamp":1789354512650,"version":"build-2803163510"},"reference-count":26,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,11,6]]},"abstract":"<jats:p>We determined the value of culture and serological tests used to diagnose histoplasmosis. The medical records of 391 histoplasmosis patients were analyzed. Diagnosis of the mycosis was assessed by culture, complement fixation, and immunodiffusion tests; 310 patients (79.5%) were male, and 184 patients (47.1%) were infected with human immunodeficiency virus (HIV). Positivity value for cultures was 35.7% (74\/207), reactivity of serological tests was 95.2% (160\/168), and a combination of both methodologies was 16.9% (35\/207) for non-HIV patients. Positivity value for cultures was 75.0% (138\/184), reactivity of serological tests was 92.4% (85\/92), and a combination of both methodologies was 26.0% (48\/184) for HIV\/acquired immunodeficiency syndrome (AIDS) patients; 48.1% (102\/212) of extrapulmonary samples from HIV\/AIDS patients yielded positive cultures compared with 23.1% (49\/212) in non-HIV patients. Lymphocyte counts made for 33.1% (61\/184) of HIV\/AIDS patients showed a trend to low CD4+ numbers and higher proportion of positive cultures. These results indicate that culture is the most reliable fungal diagnostic method for HIV\/AIDS patients, and contrary to what is generally believed, serological assays are useful for diagnosing histoplasmosis in these patients.<\/jats:p>","DOI":"10.4269\/ajtmh.13-0117","type":"journal-article","created":{"date-parts":[[2013,9,17]],"date-time":"2013-09-17T04:35:59Z","timestamp":1379392559000},"page":"937-942","source":"Crossref","is-referenced-by-count":34,"title":["Diagnostic Value of Culture and Serological Tests in the Diagnosis of Histoplasmosis in HIV and non-HIV Colombian Patients"],"prefix":"10.4269","volume":"89","author":[{"given":"Karen","family":"Arango-Bustamante","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Restrepo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz Elena","family":"Cano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catalina","family":"de Bedout","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela Maria","family":"Tob\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Med Mycol","first-page":"785","article-title":"Epidemiology of endemic systemic fungal infections in Latin America","volume":"49","author":"Colombo","year":"2002"},{"key":"R2","series-title":"Medicina UPB","first-page":"57","article-title":"Histoplasmosis cr\u00f3nica diseminada en un paciente no inmunocomprometido","volume":"21","author":"Jim\u00e9nez","year":"2002"},{"key":"R3","series-title":"Clin Microbiol Rev","first-page":"115","article-title":"Histoplasmosis: a clinical and laboratory update","volume":"20","author":"Kauffman","year":"2007"},{"key":"R4","series-title":"Semin Respir Crit Care Med","first-page":"151","article-title":"Pulmonary histoplasmosis","volume":"29","author":"Hage","year":"2008"},{"key":"R5","series-title":"Curr Opin Infect Dis","first-page":"443","article-title":"American histoplasmosis in developing countries with a special focus on patients with HIV: diagnosis, treatment and prognosis","volume":"19","author":"Couppie","year":"2006"},{"key":"R6","series-title":"Rev Iberoam Micol","first-page":"97","article-title":"Utilidad diagn\u00f3stica de la prueba intrad\u00e9rmica con histoplasmina, en \u00e1reas no end\u00e9micas de histoplasmosis","volume":"17","author":"Torres","year":"2000"},{"key":"R7","series-title":"Informe Quincenal Epidemiol Nacional","first-page":"119","article-title":"Histoplasmosis en Colombia: Estudio Interinstitucional","volume":"5","author":"Arango","year":"2000"},{"key":"R8","series-title":"Respirology","first-page":"913","article-title":"Pulmonary fungal infectons","volume":"17","author":"Smith","year":"2012"},{"key":"R9","series-title":"Clin Dermatol","first-page":"616","article-title":"Systemic mycoses in immunodepressed patients (AIDS)","volume":"30","author":"Ramos-e-Silva","year":"2012"},{"key":"R10","series-title":"Clin Infect Dis","first-page":"822","article-title":"Hospitalizations for endemic mycoses: a population-based national study","volume":"42","author":"Chu","year":"2006"},{"key":"R11","series-title":"Curr Opin Infect Dis","first-page":"421","article-title":"Diagnosis of histoplasmosis in immunosuppressed patients","volume":"21","author":"Kauffman","year":"2008"},{"key":"R12","series-title":"J Infect Dis","first-page":"1655","article-title":"Differences in histoplasmosis in patients with acquired immunodeficiency syndrome in the United States and Brazil","volume":"186","author":"Karimi","year":"2002"},{"key":"R13","series-title":"Clin Infect Dis","first-page":"1215","article-title":"Multicenter case-control study of risk factors for histoplasmosis in human immunodeficiency virus-infected people","volume":"32","author":"Hajjeh","year":"2001"},{"key":"R14","series-title":"Braz J Microbiol","first-page":"1","article-title":"Diagnosis of histoplasmosis","volume":"37","author":"Guimaraes","year":"2006"},{"key":"R15","series-title":"Infect Dis Clin North Am","first-page":"1","article-title":"Histoplasmosis","volume":"17","author":"Wheat","year":"2003"},{"key":"R16","series-title":"Expert Opin Biol Ther","first-page":"1207","article-title":"Improvements in diagnosis of histoplasmosis","volume":"6","author":"Wheat","year":"2006"},{"key":"R17","series-title":"Biomedica","first-page":"344","article-title":"Histoplasmosis: results of the Colombian national survey, 1992\u20132008","volume":"31","author":"Arango","year":"2011"},{"key":"R18","series-title":"Biomedica","first-page":"105","article-title":"Brotes de histoplasmosis registrados en el \u00e1rea andina colombiana","volume":"17","author":"Ordo\u00f1ez","year":"1997"},{"key":"R19","series-title":"Med Mycol","first-page":"57","article-title":"Histoplasmosis in Europe: report on an epidemiological survey from the European Confederation of Medical Mycology Working Group","volume":"46","author":"Ashbee","year":"2008"},{"key":"R20","series-title":"Am J Trop Med Hyg","first-page":"576","article-title":"Disseminated histoplasmosis: a comparative study between patients with acquired immunodeficiency sindrome and non-human immunodeficieny virus-infected individuals","volume":"73","author":"Tob\u00f3n","year":"2005"},{"key":"R21","series-title":"Acta Med Colomb","first-page":"277","article-title":"Histoplasmosis en el adulto","volume":"22","author":"Tob\u00f3n","year":"1997"},{"key":"R22","series-title":"AIDS","first-page":"1047","article-title":"AIDS-related Histoplasma capsulatum var. capsulatum infection: 25 years experience of French Guiana","volume":"22","author":"Huber","year":"2008"},{"key":"R23","series-title":"Med Oral Patol Oral Cir Bucal","first-page":"33","article-title":"Prevalence of oral lesions in HIV patients related to CD4 cell count and viral load in a Venezuelan population","volume":"11","author":"Bravo","year":"2006"},{"key":"R24","series-title":"J Acquir Immune Defic Syndr","first-page":"49","article-title":"Cellular immune response in HIV-infected patients with histoplasmosis","volume":"29","author":"Vail","year":"2002"},{"key":"R25","series-title":"Trends Microbiol","first-page":"488","article-title":"Current diagnosis of histoplasmosis","volume":"11","author":"Wheat","year":"2003"},{"key":"R26","series-title":"Curr Top Med Mycol","first-page":"7","article-title":"Histoplasmosis in the acquired immunodeficiency syndrome","volume":"7","author":"Wheat","year":"1996"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/89\/5\/article-p937.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/89\/5\/article-p937.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/89\/5\/article-p937.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T18:17:28Z","timestamp":1723054648000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/89\/5\/article-p937.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,6]]},"references-count":26,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.13-0117","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,6]]}}}