{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T16:02:10Z","timestamp":1757779330636},"reference-count":44,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,8,7]]},"abstract":"<jats:p>In 2012, Texas experienced the largest outbreak of human West Nile encephalitis (WNE) since the introduction of West Nile virus (WNV) in 2002. Despite the large number of WNV infections, data indicated the rate of reported WNE among human cases was no higher than in previous years. To determine whether the increase in WNV human cases could have been caused by viral genetic changes, the complete genomes of 17 isolates made from mosquito pools in Dallas and Montgomery Counties in 2012 were sequenced. The 2012 Texas isolates were found to be composed of two distinct clades, both circulating in Dallas and Montgomery Counties despite a 5-fold higher disease incidence in the former. Although minor genetic differences existed between Dallas and Montgomery WNV populations, there was weak support for population subdivision or adaptive changes. On the basis of these data, alternative explanations for increased WNV disease incidence in 2012 are proposed.<\/jats:p>","DOI":"10.4269\/ajtmh.13-0140","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T01:52:53Z","timestamp":1372729973000},"page":"205-210","source":"Crossref","is-referenced-by-count":22,"title":["Sequence Analyses of 2012 West Nile Virus Isolates from Texas Fail to Associate Viral Genetic Factors with Outbreak Magnitude"],"prefix":"10.4269","volume":"89","author":[{"given":"Nisha K.","family":"Duggal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary","family":"D'Anton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeannie","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robyn","family":"Seiferth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanne","family":"Day","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Nasci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron C.","family":"Brault","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"R1","series-title":"MMWR Morb Mortal Wkly Rep","first-page":"845","article-title":"Outbreak of West Nile-like viral encephalitis\u2013New York, 1999","volume":"48","author":"CDC","year":"1999"},{"key":"R2","series-title":"MMWR Morb Mortal Wkly Rep","first-page":"1129","article-title":"Provisional surveillance summary of the West Nile virus epidemic\u2013United States, January\u2013November 2002","volume":"51","author":"CDC","year":"2002"},{"key":"R3","series-title":"MMWR Morb Mortal Wkly Rep","first-page":"769","article-title":"West Nile virus activity\u2013United States, 2009","volume":"59","author":"CDC","year":"2010"},{"key":"R4","series-title":"MMWR Morb Mortal Wkly Rep","first-page":"1009","article-title":"West Nile virus disease and other arboviral diseases\u2013United States, 2010","volume":"60","author":"CDC","year":"2011"},{"key":"R5","series-title":"MMWR Morb Mortal Wkly Rep","first-page":"510","article-title":"West Nile virus disease and other arboviral diseases\u2013United States, 2011","volume":"61","author":"CDC","year":"2012"},{"key":"R6","series-title":"MMWR Surveill Summ","first-page":"1","article-title":"Surveillance for human West Nile virus disease\u2013United States, 1999\u20132008","volume":"59","author":"Lindsey","year":"2010"},{"key":"R7","series-title":"Epidemiol Infect","first-page":"1","article-title":"Estimated cumulative incidence of West Nile virus infection in US adults, 1999\u20132010","volume":"28","author":"Petersen","year":"2012"},{"key":"R8","series-title":"JAMA","first-page":"1846","article-title":"Record heat may have contributed to a banner year for West Nile virus. Interview with Lyle Petersen","volume":"308","author":"Petersen","year":"2012"},{"key":"R9","series-title":"J Gen Virol","first-page":"2175","article-title":"Genetic variation in West Nile virus from naturally infected mosquitoes and birds suggests quasispecies structure and strong purifying selection","volume":"86","author":"Jerzak","year":"2005"},{"key":"R10","series-title":"Virology","first-page":"143","article-title":"Diversity and evolution of West Nile virus in Illinois and the United States, 2002\u20132005","volume":"360","author":"Bertolotti","year":"2007"},{"key":"R11","series-title":"Proc Natl Acad Sci USA","first-page":"15066","article-title":"Unifying the spatial epidemiology and molecular evolution of emerging epidemics","volume":"109","author":"Pybus","year":"2012"},{"key":"R12","series-title":"Mol Phylogenet Evol","first-page":"195","article-title":"Evolutionary characterization of the West Nile Virus complete genome","volume":"56","author":"Gray","year":"2010"},{"key":"R13","series-title":"PLoS Pathog","first-page":"e1000092","article-title":"Temperature, viral genetics, and the transmission of West Nile virus by Culex pipiens mosquitoes","volume":"4","author":"Kilpatrick","year":"2008"},{"key":"R14","series-title":"Am J Trop Med Hyg","first-page":"365","article-title":"A newly emergent genotype of West Nile virus is transmitted earlier and more efficiently by Culex mosquitoes","volume":"77","author":"Moudy","year":"2007"},{"key":"R15","series-title":"Virology","first-page":"252","article-title":"Phylogenetic analysis of North American West Nile virus isolates, 2001\u20132004: evidence for the emergence of a dominant genotype","volume":"342","author":"Davis","year":"2005"},{"key":"R16","series-title":"Am J Trop Med Hyg","first-page":"493","article-title":"Genetic and phenotypic variation of West Nile virus in New York, 2000\u20132003","volume":"71","author":"Ebel","year":"2004"},{"key":"R17","series-title":"Nat Genet","first-page":"1162","article-title":"A single positively selected West Nile viral mutation confers increased virogenesis in American crows","volume":"39","author":"Brault","year":"2007"},{"key":"R18","series-title":"Mol Syst Biol","first-page":"539","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"Sievers","year":"2011"},{"key":"R19","series-title":"Mol Biol Evol","first-page":"1891","article-title":"Automated phylogenetic detection of recombination using a genetic algorithm","volume":"23","author":"Kosakovsky Pond","year":"2006"},{"key":"R20","series-title":"Nat Methods","first-page":"772","article-title":"jModelTest 2: more models, new heuristics and parallel computing","volume":"9","author":"Darriba","year":"2012"},{"key":"R21","series-title":"Syst Biol","first-page":"696","article-title":"A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood","volume":"52","author":"Guindon","year":"2003"},{"key":"R22","series-title":"Syst Biol","first-page":"307","article-title":"New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0","volume":"59","author":"Guindon","year":"2010"},{"key":"R23","series-title":"Proc Natl Acad Sci USA","first-page":"11030","article-title":"Prospects for inferring very large phylogenies by using the neighbor-joining method","volume":"101","author":"Tamura","year":"2004"},{"key":"R24","series-title":"Mol Biol Evol","first-page":"2731","article-title":"MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods","volume":"28","author":"Tamura","year":"2011"},{"key":"R25","series-title":"Bioinformatics","first-page":"2531","article-title":"Datamonkey: rapid detection of selective pressure on individual sites of codon alignments","volume":"21","author":"Pond","year":"2005"},{"key":"R26","series-title":"Mol Biol Evol","first-page":"1196","article-title":"FUBAR: A Fast, Unconstrained Bayesian AppRoximation for inferring selection","volume":"30","author":"Murrell","year":"2013"},{"key":"R27","series-title":"Emerg Infect Dis","first-page":"137","article-title":"West Nile virus infection among humans, Texas, USA, 2002\u20132011","volume":"19","author":"Nolan","year":"2013"},{"key":"R28","series-title":"Nat Rev Immunol","first-page":"687","article-title":"Evolutionary conflicts between viruses and restriction factors shape immunity.","volume":"12","author":"Duggal","year":"2012"},{"key":"R29","series-title":"Emerg Infect Dis","first-page":"785","article-title":"Evolution of new genotype of West Nile virus in North America","volume":"17","author":"McMullen","year":"2011"},{"key":"R30","series-title":"Emerg Infect Dis","first-page":"2161","article-title":"Differential virulence of West Nile strains for American crows","volume":"10","author":"Brault","year":"2004"},{"key":"R31","series-title":"PLoS Pathog","first-page":"e1000502","article-title":"RNAi targeting of West Nile virus in mosquito midguts promotes virus diversification","volume":"5","author":"Brackney","year":"2009"},{"key":"R32","series-title":"PLoS ONE","first-page":"e24466","article-title":"West Nile virus genetic diversity is maintained during transmission by Culex pipiens quinquefasciatus mosquitoes","volume":"6","author":"Brackney","year":"2011"},{"key":"R33","series-title":"Philos Trans R Soc Lond B Biol Sci","first-page":"1871","article-title":"Multi-year evolutionary dynamics of West Nile virus in suburban Chicago, USA, 2005\u20132007","volume":"365","author":"Amore","year":"2010"},{"key":"R34","series-title":"Virology","first-page":"203","article-title":"Molecular evolution of West Nile virus in a northern temperate region: Connecticut, USA 1999\u20132008","volume":"417","author":"Armstrong","year":"2011"},{"key":"R35","series-title":"Virology","first-page":"75","article-title":"Dynamic transmission of West Nile virus across the United States-Mexican border","volume":"436","author":"Mann","year":"2013"},{"key":"R36","series-title":"J Med Entomol","first-page":"309","article-title":"Effects of temperature on the transmission of West Nile virus by Culex tarsalis (Diptera: Culicidae)","volume":"43","author":"Reisen","year":"2006"},{"key":"R37","series-title":"Am J Trop Med Hyg","first-page":"884","article-title":"Effects of temperature on emergence and seasonality of West Nile virus in California","volume":"86","author":"Hartley","year":"2012"},{"key":"R38","series-title":"Science","first-page":"2333","article-title":"Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States","volume":"286","author":"Lanciotti","year":"1999"},{"key":"R39","series-title":"Science","first-page":"2331","article-title":"Isolation of West Nile virus from mosquitoes, crows, and a Cooper's hawk in Connecticut","volume":"286","author":"Anderson","year":"1999"},{"key":"R40","series-title":"Virology","first-page":"96","article-title":"Complete genome sequences and phylogenetic analysis of West Nile virus strains isolated from the United States, Europe, and the Middle East","volume":"298","author":"Lanciotti","year":"2002"},{"key":"R41","series-title":"Emerg Infect Dis","first-page":"314","article-title":"Introductions of West Nile virus strains to Mexico","volume":"12","author":"Deardorff","year":"2006"},{"key":"R42","series-title":"Virology","first-page":"220","article-title":"Phylogenetic analysis of WNV in North American blood donors during the 2003\u20132004 epidemic seasons","volume":"363","author":"Herring","year":"2007"},{"key":"R43","series-title":"Virology","first-page":"8","article-title":"Multiple pathways to the attenuation of West Nile virus in south-east Texas in 2003","volume":"405","author":"May","year":"2010"},{"key":"R44","series-title":"J Gen Virol","first-page":"2523","article-title":"North American West Nile virus genotype isolates demonstrate differential replicative capacities in response to temperature","volume":"92","author":"Andrade","year":"2011"}],"container-title":["The American Society of Tropical Medicine and 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