{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:38:13Z","timestamp":1791088693561,"version":"4.1.0"},"reference-count":14,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,9,4]]},"abstract":"<jats:p>Q fever is a widespread zoonosis caused by <jats:italic>Coxiella burnetii<\/jats:italic>, an obligate intracellular gram-negative bacterium. The investigation of <jats:italic>C. burnetii<\/jats:italic> infection in Zambian livestock was carried out using molecular detection techniques. A total of 489 cattle and 53 goat blood samples were collected from Chama, Chongwe, Monze, and Petauke districts in Zambia. Molecular screening by polymerase chain reaction was performed using <jats:italic>C. burnetii<\/jats:italic>-species-specific primers. In total, 38 cattle and 4 goat samples were positive. The prevalence of <jats:italic>C. burnetii<\/jats:italic> differed among the four sites, with Chama (Eastern province) recording the highest, although Monze (Southern province) did not record any case of the bacteria. This study reports the first genetic detection of <jats:italic>C. burnetii<\/jats:italic> in Zambia.<\/jats:p>","DOI":"10.4269\/ajtmh.13-0162","type":"journal-article","created":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T04:56:02Z","timestamp":1373950562000},"page":"518-519","source":"Crossref","is-referenced-by-count":12,"title":["First Genetic Detection of Coxiella burnetii in Zambian Livestock"],"prefix":"10.4269","volume":"89","author":[{"given":"Yongjin","family":"Qiu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Nakao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boniface","family":"Namangala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chihiro","family":"Sugimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Can Vet J","first-page":"555","article-title":"Q fever\u2014a review","volume":"31","author":"Marrie","year":"1990"},{"key":"R2","series-title":"Clin Microbiol Rev","first-page":"518","article-title":"Q fever","volume":"12","author":"Maurin","year":"1999"},{"key":"R3","series-title":"BMC Infect Dis","first-page":"280","article-title":"Assessing the long-term health impact of Q-fever in The Netherlands: a prospective cohort study started in 2007 on the largest documented Q-fever outbreak to date","volume":"12","author":"van Loenhout","year":"2012"},{"key":"R4","series-title":"Tick-Borne Diseases of Humans","first-page":"328","article-title":"Q fever","author":"Thompson","year":"2005"},{"key":"R5","series-title":"Res Vet Sci","first-page":"93","article-title":"Q fever (coxiellosis): epidemiology and pathogenesis","volume":"77","author":"Woldehiwet","year":"2004"},{"key":"R6","series-title":"S Afr Med J","first-page":"21","article-title":"Q fever in Zimbabwe. A review of the disease and the results of a serosurvey of humans, cattle, goats and dogs","volume":"83","author":"Kelly","year":"1993"},{"key":"R7","series-title":"Clin Infect Dis","first-page":"1126","article-title":"Prevalence of antibodies to Coxiella burnetii, Rickettsia conorii, and Rickettsia typhi in seven African countries","volume":"21","author":"Dupont","year":"1995"},{"key":"R8","series-title":"Vet Res Commun","first-page":"25","article-title":"Sero-prevalences of selected cattle diseases in the Kafue flats of Zambia","volume":"15","author":"Ghirotti","year":"1991"},{"key":"R9","series-title":"Am J Trop Med Hyg","first-page":"70","article-title":"Prevalence of antibodies against spotted fever, murine typhus, and Q fever rickettsiae in humans living in Zambia","volume":"61","author":"Okabayashi","year":"1999"},{"key":"R10","series-title":"Vector Borne Zoonotic Dis","first-page":"70","article-title":"The correlation of Q fever and Coxiella burnetii DNA in household environments in rural Senegal","volume":"13","author":"Ratmanov","year":"2013"},{"key":"R11","series-title":"Zentralbl Veterinarmed B","first-page":"580","article-title":"Detection of Coxiella burnetii in cow's milk using the polymerase chain reaction (PCR)","volume":"41","author":"Willems","year":"1994"},{"key":"R12","series-title":"Vet Microbiol","first-page":"285","article-title":"The detection of Coxiella burnetii from ovine genital swabs, milk and fecal samples by the use of a single touchdown polymerase chain reaction","volume":"72","author":"Berri","year":"2000"},{"key":"R13","series-title":"J Clin Microbiol","first-page":"2038","article-title":"Comparison of PCR, immunofluorescence assay, and pathogen isolation for diagnosis of Q fever in humans with spontaneous abortions","volume":"46","author":"Vaidya","year":"2008"},{"key":"R14","series-title":"Am J Trop Med Hyg","first-page":"35","article-title":"A cluster of Coxiella burnetii infections associated with exposure to vaccinated goats and their unpasteurized dairy products","volume":"47","author":"Fishbein","year":"1992"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/89\/3\/article-p518.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/89\/3\/article-p518.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/89\/3\/article-p518.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T18:24:00Z","timestamp":1723055040000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/89\/3\/article-p518.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,4]]},"references-count":14,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.13-0162","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,4]]}}}