{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T03:56:15Z","timestamp":1790567775113,"version":"4.1.0"},"reference-count":58,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,5]]},"abstract":"<jats:p>Sensitive diagnostic tools are crucial for an accurate assessment of helminth infections in low-endemicity areas. We examined stool samples from Tanzanian individuals and compared the diagnostic accuracy of a real-time polymerase chain reaction (PCR) with the FLOTAC technique and the Kato\u2013Katz method for hookworm and the Baermann method for <jats:italic>Strongyloides stercoralis<\/jats:italic> detection. Only FLOTAC had a higher sensitivity than the Kato\u2013Katz method for hookworm diagnosis; the sensitivities of PCR and the Kato\u2013Katz method were equal. PCR had a very low sensitivity for <jats:italic>S. stercoralis<\/jats:italic> detection. The cycle threshold values of the PCR were negatively correlated with the logarithm of hookworm egg and <jats:italic>S. stercoralis<\/jats:italic> larvae counts. The median larvae count was significantly lower in PCR false negatives than true positives. All methods failed to detect very low-intensity infections. New diagnostic approaches are needed for monitoring of progressing helminth control programs, confirmation of elimination, or surveillance of disease recrudescence.<\/jats:p>","DOI":"10.4269\/ajtmh.13-0268","type":"journal-article","created":{"date-parts":[[2014,1,21]],"date-time":"2014-01-21T03:35:52Z","timestamp":1390275352000},"page":"535-545","source":"Crossref","is-referenced-by-count":135,"title":["Diagnostic Accuracy of Kato\u2013Katz, FLOTAC, Baermann, and PCR Methods for the Detection of Light-Intensity Hookworm and Strongyloides stercoralis Infections in Tanzania"],"prefix":"10.4269","volume":"90","author":[{"given":"Stefanie","family":"Knopp","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahya","family":"Salim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Schindler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios A.","family":"Karagiannis Voules","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian","family":"Rothen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"Lweno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alisa S.","family":"Mohammed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond","family":"Singo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myrna","family":"Benninghoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony A.","family":"Nsojo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blaise","family":"Genton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Daubenberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Lancet","first-page":"2197","article-title":"Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 regions, 1990\u20132010: a systematic analysis for the Global Burden of Disease Study 2010","volume":"380","author":"Murray","year":"2012"},{"key":"R2","series-title":"Lancet","first-page":"981","article-title":"Antenatal anthelmintic treatment, birthweight, and infant survival in rural Nepal","volume":"364","author":"Christian","year":"2004"},{"key":"R3","series-title":"Lancet","first-page":"1521","article-title":"Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm","volume":"367","author":"Bethony","year":"2006"},{"key":"R4","series-title":"PLoS Negl Trop Dis","first-page":"e2288","article-title":"Strongyloides stercoralis: global distribution and risk factors","volume":"7","author":"Sch\u00e4r","year":"2013"},{"key":"R5","series-title":"Trans R Soc Trop Med Hyg","first-page":"967","article-title":"Strongyloidiasis\u2014the most neglected of the neglected tropical diseases?","volume":"103","author":"Olsen","year":"2009"},{"key":"R6","series-title":"PLoS Negl Trop Dis","first-page":"e1292","article-title":"Diagnosis, clinical features, and self-reported morbidity of Strongyloides stercoralis and hookworm infection in a co-endemic setting","volume":"5","author":"Becker","year":"2011"},{"key":"R7","series-title":"Infect Dis Clin North Am","first-page":"341","article-title":"Nematode infections: soil-transmitted helminths and Trichinella","volume":"26","author":"Knopp","year":"2012"},{"key":"R8","series-title":"Clin Infect Dis","first-page":"1040","article-title":"Diagnosis of Strongyloides stercoralis infection","volume":"33","author":"Siddiqui","year":"2001"},{"key":"R9","series-title":"Trans R Soc Trop Med Hyg","first-page":"314","article-title":"Strongyloides hyperinfection syndrome: an emerging global infectious disease","volume":"102","author":"Marcos","year":"2008"},{"key":"R10","series-title":"Swiss Med Wkly","first-page":"w13727","article-title":"Neglected tropical diseases: diagnosis, clinical management, treatment and control","volume":"142","author":"Utzinger","year":"2012"},{"key":"R11","series-title":"Ann Pharmacother","first-page":"1992","article-title":"Manifestations, diagnosis, and treatment of Strongyloides stercoralis infection","volume":"41","author":"Segarra-Newnham","year":"2007"},{"key":"R12","series-title":"N Engl J Med","first-page":"e15","article-title":"Images in clinical medicine. Strongyloides stercoralis embryonated ova in the lung","volume":"368","author":"Schroeder","year":"2013"},{"key":"R13","series-title":"Accelerating Work to Overcome the Global Impact of Neglected Tropical Diseases\u2014A Roadmap for Implementation","first-page":"1","author":"WHO","year":"2012"},{"key":"R14","series-title":"The London Declaration on Neglected Tropical Diseases. Table of Commitments","author":"London Declaration","year":"2012"},{"key":"R15","series-title":"PLoS Negl Trop Dis","first-page":"e1582","article-title":"A research agenda for helminth diseases of humans: the problem of helminthiases","volume":"6","author":"Lustigman","year":"2012"},{"key":"R16","series-title":"PLoS Negl Trop Dis","first-page":"e1601","article-title":"A research agenda for helminth diseases of humans: diagnostics for control and elimination programmes","volume":"6","author":"McCarthy","year":"2012"},{"key":"R17","series-title":"Parasitology","first-page":"525","article-title":"The influence of sampling effort and the performance of the Kato-Katz technique in diagnosing Schistosoma mansoni and hookworm co-infections in rural C\u00f4te d'Ivoire","volume":"127","author":"Booth","year":"2003"},{"key":"R18","series-title":"PLoS Negl Trop Dis","first-page":"e331","article-title":"Diagnosis of soil-transmitted helminths in the era of preventive chemotherapy: effect of multiple stool sampling and use of different diagnostic techniques","volume":"2","author":"Knopp","year":"2008"},{"key":"R19","series-title":"Trans R Soc Trop Med Hyg","first-page":"347","article-title":"A single FLOTAC is more sensitive than triplicate Kato-Katz for the diagnosis of low-intensity soil-transmitted helminth infections","volume":"103","author":"Knopp","year":"2009"},{"key":"R20","series-title":"PLoS Negl Trop Dis","first-page":"e754","article-title":"Comparing diagnostic accuracy of Kato-Katz, Koga agar plate, ether-concentration, and FLOTAC for Schistosoma mansoni and soil-transmitted helminths","volume":"4","author":"Glinz","year":"2010"},{"key":"R21","series-title":"Acta Trop","first-page":"185","article-title":"Accuracy of the Kato-Katz, adhesive tape and FLOTAC techniques for helminth diagnosis among children in Kyrgyzstan","volume":"116","author":"Jeandron","year":"2010"},{"key":"R22","series-title":"Parasit Vectors","first-page":"61","article-title":"Self-rated quality of life and school performance in relation to helminth infections: casestudy from Yunnan, People's Republic of China","volume":"3","author":"Ziegelbauer","year":"2010"},{"key":"R23","series-title":"Parasitol Int","first-page":"398","article-title":"Comparison of the Kato-Katz and FLOTAC techniques for the diagnosis of soil-transmitted helminth infections","volume":"60","author":"Habtamu","year":"2011"},{"key":"R24","series-title":"Acta Trop","first-page":"196","article-title":"Intestinal parasites in immigrants in the city of Naples (southern Italy)","volume":"117","author":"Gualdieri","year":"2011"},{"key":"R25","series-title":"Trans R Soc Trop Med Hyg","first-page":"84","article-title":"FLOTAC: a new sensitive technique for the diagnosis of hookworm infections in humans","volume":"102","author":"Utzinger","year":"2008"},{"key":"R26","series-title":"J Parasitol","first-page":"277","article-title":"Evaluation of three methods for laboratory diagnosis of Strongyloides 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intestinal parasites","volume":"84","author":"Taniuchi","year":"2011"},{"key":"R35","series-title":"J Clin Microbiol","first-page":"472","article-title":"A laboratory-developed TaqMan array card for simultaneous detection of 19 enteropathogens","volume":"51","author":"Liu","year":"2012"},{"key":"R36","series-title":"Diagnostic Medical Parasitology","first-page":"1","author":"Garc\u00eda","year":"2001"},{"key":"R37","series-title":"Rev Inst Med Trop Sao Paulo","first-page":"397","article-title":"A simple device for quantitative stool thick-smear technique in schistosomiasis mansoni","volume":"14","author":"Katz","year":"1972"},{"key":"R38","series-title":"Nat Protoc","first-page":"503","article-title":"FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans","volume":"5","author":"Cringoli","year":"2010"},{"key":"R39","series-title":"Trop Med Int Health","first-page":"726","article-title":"Determining the prevalence of Oesophagostomum bifurcum and Necator americanus infections using specific PCR amplification of DNA from faecal samples","volume":"6","author":"Verweij","year":"2001"},{"key":"R40","series-title":"Ann Trop Med Parasitol","first-page":"625","article-title":"Application of a circulating-cathodic-antigen (CCA) strip test and real-time PCR, in comparison with microscopy, for the detection of Schistosoma haematobium in urine samples from Ghana","volume":"102","author":"Obeng","year":"2008"},{"key":"R41","series-title":"BMC Infect Dis","first-page":"77","article-title":"Does treatment of intestinal helminth infections influence malaria? 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patients","volume":"70","author":"Hawass","year":"1997"},{"key":"R46","series-title":"Am J Epidemiol","first-page":"263","article-title":"Bayesian estimation of disease prevalence and the parameters of diagnostic tests in the absence of a gold standard","volume":"141","author":"Joseph","year":"1995"},{"key":"R47","series-title":"Biometrics","first-page":"158","article-title":"Bayesian approaches to modeling the conditional dependence between multiple diagnostic tests","volume":"57","author":"Dendukuri","year":"2001"},{"key":"R48","series-title":"Prev Vet Med","first-page":"145","article-title":"Estimation of diagnostic-test sensitivity and specificity through Bayesian modeling","volume":"68","author":"Branscum","year":"2005"},{"key":"R49","series-title":"Stat Med","first-page":"3049","article-title":"The BUGS project: evolution, critique, and future directions","volume":"28","author":"Lunn","year":"2009"},{"key":"R50","series-title":"J Comput Graph Stat","first-page":"434","article-title":"General methods for monitoring convergence of iterative simulations","volume":"7","author":"Brooks","year":"1998"},{"key":"R51","series-title":"Stat Sci","first-page":"457","article-title":"Inference from iterative simulation using multiple sequences","volume":"7","author":"Gelman","year":"1992"},{"key":"R52","series-title":"Trends Parasitol","first-page":"151","article-title":"Diagnostic dilemmas in helminthology: what tools to use and when?","volume":"25","author":"Bergquist","year":"2009"},{"key":"R53","series-title":"Acta Trop","first-page":"89","article-title":"Evaluation of real-time PCR for Strongyloides stercoralis and hookworm as diagnostic tool in asymptomatic schoolchildren in Cambodia","volume":"126","author":"Sch\u00e4r","year":"2013"},{"key":"R54","series-title":"J Helminthol","first-page":"49","article-title":"Observations on the morphology of adults and larval stages of Oesophagostomum sp. isolated from man in northern Togo and Ghana","volume":"67","author":"Blotkamp","year":"1993"},{"key":"R55","series-title":"East Afr Med J","first-page":"430","article-title":"Hookworm studies in Uganda: investigations at Teboke in Lango District","volume":"43","author":"Sturrock","year":"1966"},{"key":"R56","series-title":"East Afr Med J","first-page":"315","article-title":"Hookworm studies in Tanganyika (Tanzania): investigations at Hombolo in the Dodoma region","volume":"43","author":"Sturrock","year":"1966"},{"key":"R57","series-title":"Ann Trop Med Parasitol","first-page":"147","article-title":"Hookworm species in patients from Kenyatta National Hospital Nairobi","volume":"80","author":"Chunge","year":"1986"},{"key":"R58","series-title":"PLoS Negl Trop Dis","first-page":"e1036","article-title":"Diagnostic accuracy of Kato-Katz and FLOTAC for assessing anthelmintic drug efficacy","volume":"5","author":"Knopp","year":"2011"}],"container-title":["The American Society of Tropical Medicine and 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