{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:39:36Z","timestamp":1791196776560,"version":"4.1.0"},"reference-count":42,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,8]]},"abstract":"<jats:p>The <jats:italic>Giardia<\/jats:italic> and <jats:italic>Cryptosporidium<\/jats:italic> species are widespread and frequent diarrhea-related parasites affecting humans and other mammalian species. The prevalence of these parasites in Mongolia is currently unknown. Therefore, we performed molecular analyses of <jats:italic>G. duodenalis<\/jats:italic> and <jats:italic>C. parvum<\/jats:italic> in stool samples from 138 patients hospitalized with diarrhea in Mongolia using nested polymerase chain reaction (PCR). A total of 5 (3.62%) and 7 (5.07%) fecal samples were positive for <jats:italic>G. duodenalis<\/jats:italic> and <jats:italic>C. parvum<\/jats:italic>, respectively. <jats:italic>Giardia duodenalis<\/jats:italic> and <jats:italic>C. parvum<\/jats:italic> infections were prevalent in children &lt; 9 years of age. The assemblage-specific fragment patterns for the \u03b2-giardin gene of <jats:italic>G. duodenalis<\/jats:italic> revealed that all five samples testing positive belonged to Assemblage A by the PCR-restriction fragment polymorphism method. For sequencing and phylogenetic analysis of the 18S rDNA and HSP70 genes of all seven patients testing positive the genes were further identified to be of the <jats:italic>C. parvum<\/jats:italic> bovine genotype. This study is the first to report the prevalence of <jats:italic>G. duodenalis<\/jats:italic> and <jats:italic>C. parvum<\/jats:italic> and its molecular characterization of fecal samples from individuals with diarrhea in Mongolia.<\/jats:p>","DOI":"10.4269\/ajtmh.13-0271","type":"journal-article","created":{"date-parts":[[2013,11,19]],"date-time":"2013-11-19T04:59:52Z","timestamp":1384837192000},"page":"43-47","source":"Crossref","is-referenced-by-count":12,"title":["Molecular Characterization of Giardia duodenalis and Cryptosporidium parvum in Fecal Samples of Individuals in Mongolia"],"prefix":"10.4269","volume":"90","author":[{"given":"Sung-Hee","family":"Hong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davaasuren","family":"Anu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Il","family":"Jeong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davaajav","family":"Abmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin-Hyeong","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won-Ja","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang-Eun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Int J Parasitol","first-page":"1239","article-title":"Molecular characterization of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission","volume":"38","author":"Xiao","year":"2008"},{"key":"R2","series-title":"Exp Parasitol","first-page":"80","article-title":"Molecular epidemiology of cryptosporidiosis: an update","volume":"30","author":"Xiao","year":"2000"},{"key":"R3","series-title":"Int J Parasitol","first-page":"1259","article-title":"Giardiasis as a re-emerging infectious disease and its zoonotic potential.","volume":"30","author":"Thompson","year":"2000"},{"key":"R4","series-title":"Parasitic Protozoa","first-page":"225","article-title":"Giardia in humans and other animals","author":"Kulda","year":"1995"},{"key":"R5","series-title":"Clin Microbiol Rev","first-page":"447","article-title":"Biology of Giardia lamblia","volume":"14","author":"Adam","year":"2001"},{"key":"R6","series-title":"J Clin Microbiol","first-page":"446","article-title":"Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human feces","volume":"40","author":"Amar","year":"2002"},{"key":"R7","series-title":"Int J Parasitol","first-page":"229","article-title":"Correlation between genotype of Giardia duodenalis and diarrhoea","volume":"32","author":"Read","year":"2002"},{"key":"R8","series-title":"PLoS Negl Trop Dis","first-page":"e558","article-title":"ZOOPNET network and partners: identification of zoonotic genotypes of Giardia duodenalis","volume":"3","author":"Sprong","year":"2009"},{"key":"R9","series-title":"Infect Genet Evol","first-page":"29","article-title":"Genetic diversity within the morphological species Giardia intestinalis and its relationship to host origin","volume":"3","author":"Monis","year":"2003"},{"key":"R10","series-title":"Mol Biochem Parasitol","first-page":"75","article-title":"Molecular epidemiology of giardiasis","volume":"40","author":"Cacci\u00f2","year":"2010"},{"key":"R11","series-title":"Vet Parasitol","first-page":"197","article-title":"High prevalence of Giardia detected in cats by PCR","volume":"110","author":"McGlade","year":"2003"},{"key":"R12","series-title":"Int J Parasitol","first-page":"1063","article-title":"The identification of a new Giardia duodenalis assemblage in marine vertebrates and a preliminary analysis of G. duodenalis population biology in marine systems","volume":"40","author":"Lasek-Nesselquist","year":"2010"},{"key":"R13","series-title":"J Water Health","first-page":"1","article-title":"Waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt","volume":"5","author":"Karanis","year":"2007"},{"key":"R14","series-title":"Clin Microbiol Rev","first-page":"110","article-title":"Zoonotic potential and molecular epidemiology of Giardia species and giardiasis","volume":"24","author":"Feng","year":"2011"},{"key":"R15","series-title":"Int J Parasitol","first-page":"1305","article-title":"Epidemiology of Cryptosporidium: transmission, detection and identification","volume":"30","author":"Fayer","year":"2000"},{"key":"R16","series-title":"The World Health Report","author":"WHO","year":"1996"},{"key":"R17","series-title":"Emerg Infect Dis","first-page":"607","article-title":"Food-related illness and death in the United States","volume":"5","author":"Mead","year":"1999"},{"key":"R18","series-title":"Environ Res","first-page":"260","article-title":"Occurrence of Giardia and Cryptosporidium in water supplies of Russia and Bulgaria","volume":"102","author":"Karanis","year":"2006"},{"key":"R19","series-title":"Exp Parasitol","first-page":"42","article-title":"Genetic characterizations of Cryptosporidium spp. and Giardia duodenalis in humans in Henan, China","volume":"127","author":"Wang","year":"2011"},{"key":"R20","series-title":"Int J Parasitol","first-page":"1023","article-title":"Sequence analysis of the \u03b2-giardin gene and development of a polymerase chain reaction\u2013restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human fecal samples","volume":"32","author":"Cacci\u00f2","year":"2002"},{"key":"R21","series-title":"Pharmacogenomics","first-page":"353","article-title":"A capillary electrophoresis method for genotyping the 9-bp exon 1 insertion\/deletion in BDKRB2","volume":"13","author":"Talameh","year":"2012"},{"key":"R22","series-title":"Int J Parasitol","first-page":"207","article-title":"Genetic heterogeneity at the \u03b2-giardin locus among human and animal isolates of Giardia duodenalis and identification of potentially zoonotic subgenotypes","volume":"35","author":"Lalle","year":"2005"},{"key":"R23","series-title":"Int J Parasitol","first-page":"289","article-title":"Molecular and phylogenetic characterization of Cryptosporidium from birds","volume":"31","author":"Morgan","year":"2001"},{"key":"R24","series-title":"J Eukaryot Microbiol","first-page":"416","article-title":"Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form Hsp70","volume":"42","author":"Khramtsov","year":"1995"},{"key":"R25","series-title":"Mol Biol Evol","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987"},{"key":"R26","series-title":"Brief Bioinform","first-page":"299","article-title":"MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences","volume":"9","author":"Kumar","year":"2008"},{"key":"R27","series-title":"Evolution","first-page":"783","article-title":"Confidence limits on phylogenies: an approach using the bootstrap","volume":"39","author":"Felsenstein","year":"1985"},{"key":"R28","series-title":"Southeast Asian J Trop Med Public Health","first-page":"991","article-title":"Prevalence rates of Giardia and Cryptosporidium among diarrheic patients in the Philippines","volume":"39","author":"Natividad","year":"2008"},{"key":"R29","series-title":"Med J Malaysia","first-page":"296","article-title":"Intestinal parasitic infections in man: a review","volume":"58","author":"Norhayati","year":"2003"},{"key":"R30","series-title":"Pol J Microbiol","first-page":"259","article-title":"Detection of Giardia intestinalis assemblages A, B and D in domestic cats from Warsaw, Poland","volume":"60","author":"Jaros","year":"2011"},{"key":"R31","series-title":"Euro Surveill","first-page":"19642","article-title":"Laboratory-based surveillance for Cryptosporidium in France, 2006\u20132009","volume":"15","author":"ANOFEL Cryptosporidium National Network","year":"2010"},{"key":"R32","series-title":"Clin Microbiol Rev","first-page":"35","article-title":"Immune Response to Giardia duodenalis","volume":"13","author":"Faubert","year":"2000"},{"key":"R33","series-title":"Mol Biochem Parasitol","first-page":"73","article-title":"Classification of subgroups of Giardia lamblia based upon ribosomal RNA gene sequence using the polymerase chain reaction","volume":"54","author":"Weiss","year":"1992"},{"key":"R34","series-title":"Mol Biochem Parasitol","first-page":"75","article-title":"Molecular epidemiology of giardiasis","volume":"160","author":"Cacci\u00f2","year":"2008"},{"key":"R35","series-title":"Southeast Asian J Trop Med Public Health","first-page":"764","article-title":"Determination of discriminatory power of genetic markers used for genotyping Giardia duodenalis","volume":"42","author":"Siripattanapipong","year":"2011"},{"key":"R36","series-title":"Parasitol Res","first-page":"681","article-title":"Genotyping of Giardia duodenalis isolates among residents of slum area in Manila, Philippines","volume":"101","author":"Yason","year":"2007"},{"key":"R37","series-title":"J Parasitol","first-page":"887","article-title":"Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA Sequences","volume":"86","author":"Yong","year":"2000"},{"key":"R38","series-title":"J Vet Med Sci","first-page":"337","article-title":"Identification of genotypes of Giardia intestinalis isolates from a human and calf in Japan","volume":"67","author":"Matsubayashi","year":"2005"},{"key":"R39","series-title":"J Egypt Soc Parasitol","first-page":"547","article-title":"Genetic characterization of Giardia lamblia isolates from Egyptian patients with relation to clinical giardiasis","volume":"38","author":"Abdel-Moneim","year":"2008"},{"key":"R40","series-title":"Acta Trop","first-page":"10","article-title":"Genotyping of Giardia duodenalis from human and animal samples from Brazil using \u03b2-giardin gene: a phylogenetic analysis","volume":"102","author":"Volot\u00e3o","year":"2007"},{"key":"R41","series-title":"Int J Parasitol","first-page":"1007","article-title":"Cryptosporidium molnari n. sp. 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