{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T17:10:28Z","timestamp":1790442628810,"version":"4.1.0"},"reference-count":26,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,6,4]]},"abstract":"<jats:p>This study describes our investigation on the prevalence and molecular identification of bartonellae from <jats:italic>Rattus diardii<\/jats:italic> and <jats:italic>R. norvegicus<\/jats:italic> in the urban areas of Malaysia. Of 95 rats investigated, <jats:italic>Bartonella tribocorum<\/jats:italic>, <jats:italic>B. rattimassiliensis<\/jats:italic>, <jats:italic>B. coopersplainsensis<\/jats:italic>, <jats:italic>B. elizabethae<\/jats:italic>, and <jats:italic>B. queenslandensis<\/jats:italic> were isolated from kidney and spleen homogenates of four rats. Bartonellae DNA was amplified from the rat organ tissues by using primers specific for the bartonellae RNA polymerase beta subunit (<jats:italic>rpo<\/jats:italic>B) gene in nine other rats. Sequence analysis of the <jats:italic>rpo<\/jats:italic>B gene fragments shows the identification of <jats:italic>B. queenslandensis<\/jats:italic> in five rats, <jats:italic>B. elizabethae<\/jats:italic> in three rats, and <jats:italic>B. tribocorum<\/jats:italic> in one rat. Combining the results of isolation and molecular detection of bartonellae, we found that the prevalence of <jats:italic>Bartonella<\/jats:italic> infection in the <jats:italic>Rattus<\/jats:italic> spp. investigated in this study was 13.7%. Implementation of effective rat control program in the urban areas is necessary to prevent the spillover of bartonellosis from rats to humans.<\/jats:p>","DOI":"10.4269\/ajtmh.13-0273","type":"journal-article","created":{"date-parts":[[2014,4,15]],"date-time":"2014-04-15T04:20:10Z","timestamp":1397535610000},"page":"1039-1042","source":"Crossref","is-referenced-by-count":16,"title":["Isolation and Molecular Identification of Bartonellae from Wild Rats (Rattus Species) in Malaysia"],"prefix":"10.4269","volume":"90","author":[{"given":"Sun Tee","family":"Tay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aida Syafinaz","family":"Mokhtar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siti Nursheena Mohd","family":"Zain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiat 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infections","volume":"301","author":"Kaiser","year":"2011"},{"key":"R5","series-title":"Am J Trop Med Hyg","first-page":"1140","article-title":"Identification of Bartonella infections in febrile human patients from Thailand and their potential animal reservoirs","volume":"82","author":"Kosoy","year":"2010"},{"key":"R6","series-title":"Am J Trop Med Hyg","first-page":"567","article-title":"Bartonella strains in small mammals from Dhaka, Bangladesh, related to Bartonella in America and Europe","volume":"77","author":"Bai","year":"2007"},{"key":"R7","series-title":"Am J Trop Med Hyg","first-page":"622","article-title":"Genetic and ecologic characteristics of Bartonella communities in rodents in southern China","volume":"66","author":"Ying","year":"2002"},{"key":"R8","series-title":"Southeast Asian J Trop Med Public Health","first-page":"1523","article-title":"Bartonella species in rodents and shrews in the greater Jakarta area","volume":"36","author":"Winoto","year":"2005"},{"key":"R9","series-title":"Appl Environ Microbiol","first-page":"5086","article-title":"Prevalence and genetic diversity of Bartonella species isolated from wild rodents in Japan","volume":"74","author":"Inoue","year":"2008"},{"issue":"(Suppl 2)","key":"R10","series-title":"Clin Microbiol Infect","first-page":"95","article-title":"Molecular detection of Bartonella species in rodents from the Lao PDR","volume":"15","author":"Angelakis","year":"2009"},{"key":"R11","series-title":"Appl Environ Microbiol","first-page":"8463","article-title":"Prevalence and genetic diversity of Bartonella spp. in small mammals from Southeastern Asia","volume":"78","author":"Jiyipong","year":"2012"},{"key":"R12","series-title":"J Med Microbiol","first-page":"1496","article-title":"Isolation of Bartonella species from rodents in Taiwan including a strain closely related to \u2018Bartonella rochalimae\u2019 from Rattus norvegicus","volume":"57","author":"Lin","year":"2008"},{"key":"R13","series-title":"Appl Environ Microbiol","first-page":"8247","article-title":"Prevalence and genetic diversity of Bartonella species detected in different tissues of small mammals in Nepal","volume":"76","author":"Gundi","year":"2010"},{"key":"R14","series-title":"Am J Trop Med Hyg","first-page":"811","article-title":"Prevalence and genetic heterogeneity of Bartonella strains cultured from rodents from 17 provinces in Thailand","volume":"81","author":"Bai","year":"2009"},{"key":"R15","series-title":"Am J Trop Med Hyg","first-page":"429","article-title":"Prevalence and diversity of Bartonella in rodents of northern Thailand: a comparison with Bartonella in rodents from southern China","volume":"70","author":"Castle","year":"2004"},{"key":"R16","series-title":"J Med Assoc Thai","first-page":"707","article-title":"Emerging Bartonella in humans and animals in Asia and 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