{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:29:47Z","timestamp":1790429387506,"version":"4.1.0"},"reference-count":45,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,5]]},"abstract":"<jats:p><jats:italic>Burkholderia pseudomallei<\/jats:italic> causes melioidosis, the third most common cause of death from infectious diseases in northeast Thailand. Four physicochemical factors were set so that their values covered the range of the northeast, which is an endemic area. The soil pH was set at pH 4\u201310, soil salinity was 0.0\u20135.0% NaCl, total iron was 50\u2013150 mg\/kg soil, and carbon to nitrogen ratio (C\/N) was 10:1 to 40:1. The experiments were carried out at 37\u00b0C, and soil moisture was maintained for 7 days. The number of viable bacterial cells was counted daily. Soil pH, salinity, Fe, and C\/N ratio affected the bacterial growth. The bacterial colony was significantly (<jats:italic>P<\/jats:italic> &lt; 0.05) reduced at soil pH &gt; 8, soil salinity &gt; 1% NaCl, and C\/N ratio &gt; 40:1. However, the growth of <jats:italic>B. pseudomallei<\/jats:italic> was enhanced by increasing the concentrations of iron significantly (<jats:italic>P<\/jats:italic> &lt; 0.05). We propose using these findings to control <jats:italic>B. pseudomallei in situ<\/jats:italic>.<\/jats:p>","DOI":"10.4269\/ajtmh.13-0446","type":"journal-article","created":{"date-parts":[[2014,1,21]],"date-time":"2014-01-21T03:35:52Z","timestamp":1390275352000},"page":"480-485","source":"Crossref","is-referenced-by-count":40,"title":["Physicochemical Factors Affecting the Growth of Burkholderia pseudomallei in Soil Microcosm"],"prefix":"10.4269","volume":"90","author":[{"given":"Supunnipa","family":"Wang-ngarm","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sorujsiri","family":"Chareonsudjai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pisit","family":"Chareonsudjai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Acta Trop","first-page":"129","article-title":"Melioidosis in Southeast Asia","volume":"74","author":"Leelarasamee","year":"2000"},{"key":"R2","series-title":"Lancet","first-page":"1715","article-title":"Melioidosis","volume":"361","author":"White","year":"2003"},{"key":"R3","series-title":"Bioterrorism Agents\/Diseases","author":"Centers for Disease Control and Prevention","year":"2013"},{"key":"R4","series-title":"Am J Trop Med Hyg","first-page":"177","article-title":"A cluster of melioidosis cases from an endemic region is clonal and is linked to the water supply using molecular typing of Burkholderia pseudomallei isolates","volume":"65","author":"Currie","year":"2001"},{"key":"R5","series-title":"PLoS Negl Trop Dis","first-page":"e900","article-title":"The epidemiology and clinical spectrum of melioidosis: 540 cases from the 20 year Darwin prospective study","volume":"4","author":"Currie","year":"2010"},{"key":"R6","series-title":"Epidemiol Infect","first-page":"437","article-title":"Acute melioidosis outbreak in Western Australia","volume":"123","author":"Inglis","year":"1999"},{"key":"R7","series-title":"Infect Immun","first-page":"3332","article-title":"Natural history of inhalation melioidosis in rhesus macaques (Macaca mulatta) and African green monkeys (Chlorocebus aethiops)","volume":"80","author":"Yeager","year":"2012"},{"key":"R8","series-title":"Med J Aust","first-page":"364","article-title":"Tsunami lung: a necrotising pneumonia in survivors of the Asian tsunami","volume":"182","author":"Allworth","year":"2005"},{"key":"R9","series-title":"Clin Infect Dis","first-page":"982","article-title":"Melioidosis in 6 tsunami survivors in southern Thailand","volume":"41","author":"Chierakul","year":"2005"},{"key":"R10","series-title":"Clin Infect Dis","first-page":"896","article-title":"Pneumonia associated with near-drowning","volume":"25","author":"Ender","year":"1997"},{"key":"R11","series-title":"Clin Microbiol Rev","first-page":"383","article-title":"Melioidosis: epidemiology, pathophysiology, and management","volume":"18","author":"Cheng","year":"2005"},{"key":"R12","series-title":"Clin Microbiol Rev","first-page":"52","article-title":"Melioidosis: the tip of the iceberg?","volume":"4","author":"Dance","year":"1991"},{"key":"R13","series-title":"Trends Microbiol","first-page":"138","article-title":"The many facets of melioidosis","volume":"7","author":"Dharakul","year":"1999"},{"key":"R14","series-title":"Trans R Soc Trop Med Hyg","first-page":"488","article-title":"Quantitative recovery of Burkholderia pseudomallei from soil in Thailand","volume":"89","author":"Smith","year":"1995"},{"key":"R15","series-title":"J Med Assoc Thai","first-page":"345","article-title":"Community-acquired pneumonia in adults at Srinagarind Hospital","volume":"73","author":"Boonsawat","year":"1990"},{"key":"R16","series-title":"J Infect Dis","first-page":"890","article-title":"Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand","volume":"159","author":"Chaowagul","year":"1989"},{"key":"R17","series-title":"Am J Trop Med Hyg","first-page":"458","article-title":"Epidemiology of Burkholderia pseudomallei in Thailand","volume":"60","author":"Vuddhakul","year":"1999"},{"key":"R18","series-title":"Southeast Asian J Trop Med Public Health","first-page":"107","article-title":"Isolation rates of Burkholderia pseudomallei among the four regions in Thailand","volume":"28","author":"Leelarasamee","year":"1997"},{"key":"R19","series-title":"Med J Aust","first-page":"314","article-title":"Meliodosis","volume":"1","author":"Ashdown","year":"1980"},{"key":"R20","series-title":"Acta Trop","first-page":"121","article-title":"The epidemiology of melioidosis in Australia and Papua New Guinea","volume":"74","author":"Currie","year":"2000"},{"key":"R21","series-title":"Commun Dis Intell","first-page":"444","article-title":"Melioidosis in north Queensland, 2000\u20132009","volume":"34","author":"Hanna","year":"2010"},{"key":"R22","series-title":"Trans R Soc Trop Med Hyg","first-page":"856","article-title":"Clinical presentation of melioidosis in Queensland, Australia","volume":"99","author":"Malczewski","year":"2005"},{"key":"R23","series-title":"Am J Trop Med Hyg","first-page":"1113","article-title":"Increasing incidence of human melioidosis in Northeast Thailand","volume":"82","author":"Limmathurotsakul","year":"2010"},{"key":"R24","series-title":"PLoS One","first-page":"e12485","article-title":"Defining the true sensitivity of culture for the diagnosis of melioidosis using Bayesian latent class models","volume":"5","author":"Limmathurotsakul","year":"2010"},{"issue":"(Suppl 1)","key":"R25","series-title":"Trans R Soc Trop Med Hyg","first-page":"S5","article-title":"Soil physicochemical properties related to the presence of Burkholderia pseudomallei","volume":"102","author":"Palasatien","year":"2008"},{"key":"R26","series-title":"PLoS Negl Trop Dis","first-page":"e364","article-title":"Landscape changes influence the occurrence of the melioidosis bacterium Burkholderia pseudomallei in soil in northern Australia","volume":"3","author":"Kaestli","year":"2009"},{"key":"R27","series-title":"Am J Trop Med Hyg","first-page":"759","article-title":"Short report: a rapid method for the differentiation of Burkholderia pseudomallei and Burkholderia thailandensis","volume":"66","author":"Wuthiekanun","year":"2002"},{"key":"R28","series-title":"Trans R Soc Trop Med Hyg","first-page":"346","article-title":"Improved culture-based detection and quantification of Burkholderia pseudomallei from soil","volume":"105","author":"Trung","year":"2011"},{"key":"R29","series-title":"Folia Microbiol (Praha)","first-page":"253","article-title":"Effects of soil pH, temperature and water content on the growth of Burkholderia pseudomallei","volume":"48","author":"Chen","year":"2003"},{"key":"R30","series-title":"J Microbiol Methods","first-page":"121","article-title":"How to optimize the drop plate method for enumerating bacteria","volume":"44","author":"Herigstad","year":"2001"},{"key":"R31","series-title":"Methods of Soil Analysis, Part 3: Chemical Methods","first-page":"475","article-title":"Soil pH and soil acidity","author":"Thomas","year":"1996"},{"key":"R32","series-title":"Methods of Soil Analysis, Part 3: Chemical Methods","first-page":"417","article-title":"Salinity: electrical conductivity and total dissolved solids","author":"Rhoades","year":"1996"},{"key":"R33","series-title":"Laboratory Guide for Conducting Soil Tests and Plant Analysis","author":"Jones","year":"1930"},{"key":"R34","series-title":"Soil Sci","first-page":"251","article-title":"An examination of the Degtjareff method for determining organic carbon in soils: effect of variations in digestion conditions and of inorganic soil constituents","volume":"63","author":"Walkley","year":"1934"},{"key":"R35","series-title":"Appl Environ Microbiol","first-page":"1246","article-title":"Comparison of the pour, spread, and drop plate methods for enumeration of Rhizobium spp. in inoculants made from presterilized peat","volume":"44","author":"Hoben","year":"1982"},{"key":"R36","series-title":"Appl Environ Microbiol","first-page":"6865","article-title":"Environmental factors that affect the survival and persistence of Burkholderia pseudomallei","volume":"72","author":"Inglis","year":"2006"},{"key":"R37","series-title":"Microbiol Immunol","first-page":"451","article-title":"Laboratory investigation of ecological factors influencing the environmental presence of Burkholderia pseudomallei","volume":"40","author":"Tong","year":"1996"},{"key":"R38","series-title":"Jpn J Med Sci Biol","first-page":"63","article-title":"Growth and survival of Pseudomonas pseudomallei in acidic environments","volume":"44","author":"Dejsirilert","year":"1991"},{"key":"R39","series-title":"Document for the Seminar on \u201cHelp Isan Fight Saline Soil.\u201d","author":"Department of Land Development","year":"1995"},{"key":"R40","series-title":"Agron J","first-page":"39","article-title":"Crop growth and salinity","volume":"17","author":"Bernstein","year":"1974"},{"key":"R41","series-title":"Field Crop Res","first-page":"289","article-title":"Yield of rice under water and soil salinity risks in farmers fields in northeast Thailand","volume":"118","author":"Clermont Dauphin","year":"2010"},{"key":"R42","series-title":"Soil Biol Biochem","first-page":"2075","article-title":"Gross N transformation rates and net N mineralisation rates related to the C and N contents of soil organic matter fractions in grassland soils of different age","volume":"36","author":"Accoe","year":"2004"},{"key":"R43","series-title":"Appl Environ Microbiol","first-page":"5305","article-title":"Association of the melioidosis agent Burkholderia pseudomallei with water parameters in rural water supplies in Northern Australia","volume":"76","author":"Draper","year":"2010"},{"key":"R44","series-title":"Infect Immun","first-page":"776","article-title":"Siderophore production by Pseudomonas pseudomallei","volume":"59","author":"Yang","year":"1991"},{"key":"R45","series-title":"Appl Environ Microbiol","first-page":"6891","article-title":"Sensitive and specific molecular detection of Burkholderia pseudomallei, the causative agent of melioidosis, in the soil of tropical northern Australia","volume":"73","author":"Kaestli","year":"2007"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/90\/3\/article-p480.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/90\/3\/article-p480.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/90\/3\/article-p480.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T21:32:00Z","timestamp":1723066320000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/90\/3\/article-p480.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,5]]},"references-count":45,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.13-0446","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,5]]}}}