{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T20:10:06Z","timestamp":1793909406280,"version":"4.8.0"},"reference-count":26,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,2,5]]},"abstract":"<jats:p>The zoonotic potential of <jats:italic>Cryptosporidium parvum<\/jats:italic> was studied in an extensive cattle farming region of northern Tunisia. Seventy fecal samples from pre-weaning calves and 403 fecal samples from children were examined by microscopy after modified Ziehl\u2013Neelsen (MZN) staining. Positive <jats:italic>Cryptosporidium<\/jats:italic> specimens were identified at a species level using an 18S rRNA nested polymerase chain reaction (PCR) followed by an Restriction Fragment Length Polymorphism (RFLP) analysis. <jats:italic>C. parvum<\/jats:italic> isolates were subgenotyped by sequence analysis of the glycoprotein 60 (gp60) gene. Among calf samples, 14 samples were positive by MZN method. <jats:italic>C. parvum<\/jats:italic> was identified in all cases. Twelve <jats:italic>parvum<\/jats:italic> isolates (85.7%) belonged to family subtype IIa. Subtype IIaA15G2R1 was more prevalent (50%). Two <jats:italic>C. parvum<\/jats:italic> isolates corresponded to the IIdA16G1 subtype. Seven human samples were positive by MZN method. <jats:italic>C. parvum<\/jats:italic> and <jats:italic>C. meleagridis<\/jats:italic> were identified in four and three cases, respectively. Intraspecific characterization of <jats:italic>C. parvum<\/jats:italic> identified two subtypes, the IIaA15G2R1 and the IIdA16G1, also found in calves.<\/jats:p>","DOI":"10.4269\/ajtmh.13-0522","type":"journal-article","created":{"date-parts":[[2013,12,17]],"date-time":"2013-12-17T04:51:49Z","timestamp":1387255909000},"page":"346-350","source":"Crossref","is-referenced-by-count":33,"title":["Glycoprotein 60 Diversity in Cryptosporidium parvum Causing Human and Cattle Cryptosporidiosis in the Rural Region of Northern Tunisia"],"prefix":"10.4269","volume":"90","author":[{"given":"Ikram","family":"Rahmouni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rym","family":"Essid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karim","family":"Aoun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A\u00efda","family":"Bouratbine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Parasitol Res","first-page":"287","article-title":"Distribution of Cryptosporidium subtypes in human and domestic and wild ruminants in Portugal","volume":"99","author":"Alves","year":"2006"},{"key":"R2","series-title":"Trends Parasitol","first-page":"237","article-title":"Looking for Cryptosporidium: the application of advances in detection and 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