{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T08:52:54Z","timestamp":1790153574043,"version":"4.0.1"},"reference-count":41,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,4,2]]},"abstract":"<jats:p>Haitian children were monitored longitudinally in a filariasis study. Included were stool samples examined for <jats:italic>Giardia intestinalis<\/jats:italic> and <jats:italic>Entamoeba histolytica<\/jats:italic> cysts, and serum specimens analyzed for immunoglobulin G (IgG) responses to eight recombinant antigens from <jats:italic>G. intestinalis<\/jats:italic> (variant-specific surface protein [VSP1\u2013VSP5]), <jats:italic>E. histolytica<\/jats:italic> (lectin adhesion molecule [LecA]), and <jats:italic>Cryptosporidium parvum<\/jats:italic> (17- and 27-kDa) using a multiplex bead assay. The IgG responses to VSP antigens peaked at 2 years of age and then diminished and were significantly lower (<jats:italic>P<\/jats:italic> &lt; 0.002) in children &gt; 4.5 years than in children &lt; 4.5 years. The IgG responses to <jats:italic>Cryptosporidium<\/jats:italic> tended to increase with age. The IgG responses to LecA and VSP antigens and the prevalence of stools positive for cysts were significantly higher (<jats:italic>P<\/jats:italic> &lt; 0.037 and <jats:italic>P<\/jats:italic> &lt; 0.035, respectively) in the rainy season than in the dry season. The multiplex bead assay provides a powerful tool for analyzing serologic responses to multiple pathogens.<\/jats:p>","DOI":"10.4269\/ajtmh.13-0545","type":"journal-article","created":{"date-parts":[[2014,3,4]],"date-time":"2014-03-04T04:42:51Z","timestamp":1393908171000},"page":"653-660","source":"Crossref","is-referenced-by-count":39,"title":["Longitudinal Evaluation of Enteric Protozoa in Haitian Children by Stool Exam and Multiplex Serologic Assay"],"prefix":"10.4269","volume":"90","author":[{"given":"Delynn M.","family":"Moss","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey 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mortality","volume":"8","author":"Walsh","year":"1986"},{"key":"R2","series-title":"Annu Rev Public Health","first-page":"135","article-title":"Environmental ecology of Cryptosporidium and public health implications","volume":"18","author":"Rose","year":"1997"},{"key":"R3","series-title":"Parasitol Res","first-page":"681","article-title":"Genotyping of Giardia duodenalis isolates among residents of slum area in Manila, Philippines","volume":"101","author":"Yason","year":"2007"},{"key":"R4","series-title":"Am J Trop Med Hyg","first-page":"6","article-title":"Seroepidemiology of giardiasis in Mexico","volume":"80","author":"Cedillo-Rivera","year":"2009"},{"key":"R5","series-title":"J Clin Microbiol","first-page":"3235","article-title":"Diagnosis of amebic liver abscess and intestinal infection with the TechLab Entamoeba histolytica II antigen detection and antibody tests","volume":"38","author":"Haque","year":"2000"},{"key":"R6","series-title":"J Clin Microbiol","first-page":"4569","article-title":"Evaluation of Entamoeba histolytica antigen and antibody point-of-care tests for the rapid diagnosis of amebiasis","volume":"44","author":"Leo","year":"2006"},{"key":"R7","series-title":"Am J Trop Med Hyg","first-page":"110","article-title":"Enzyme-linked immunoelectrotransfer blot analysis of a cryptosporidiosis outbreak on a United States Coast Guard cutter","volume":"58","author":"Moss","year":"1998"},{"key":"R8","series-title":"J Infect Dis","first-page":"827","article-title":"The antibody response to 27-, 17-, and 15-kDa Cryptosporidium antigens following experimental infection in humans","volume":"178","author":"Moss","year":"1998"},{"key":"R9","series-title":"J Parasitol","first-page":"397","article-title":"Detection of Cryptosporidium antibodies in sera and oral fluids using multiplex bead assay","volume":"90","author":"Moss","year":"2004"},{"key":"R10","series-title":"J Clin Microbiol","first-page":"5298","article-title":"Changes in serum immunoglobulin G levels as a marker for Cryptosporidium sp. infection in Peruvian children","volume":"43","author":"Priest","year":"2005"},{"key":"R11","series-title":"Clin Vaccine Immunol","first-page":"123","article-title":"Longitudinal analysis of Cryptosporidium species-specific immunoglobulin G antibody responses in Peruvian children","volume":"13","author":"Priest","year":"2006"},{"key":"R12","series-title":"J Clin Microbiol","first-page":"1385","article-title":"Detection by enzyme immunoassay of serum immunoglobulin G antibodies that recognize specific Cryptosporidium parvum antigens","volume":"37","author":"Priest","year":"1999"},{"key":"R13","series-title":"Clin Vaccine Immunol","first-page":"1695","article-title":"Multiplex assay detection of immunoglobulin G antibodies that recognize Giardia intestinalis and Cryptosporidium parvum antigens","volume":"17","author":"Priest","year":"2010"},{"key":"R14","series-title":"Trans R Soc Trop Med Hyg","first-page":"656","article-title":"Bancroftian filariasis: effect of repeated treatment with diethylcarbamazine and albendazole on microfilaraemia, antigenaemia and antifilarial antibodies","volume":"100","author":"Helmy","year":"2006"},{"key":"R15","series-title":"Am J Trop Med Hyg","first-page":"217","article-title":"Longitudinal analysis of the development of filarial infection and antifilarial immunity in a cohort of Haitian children","volume":"59","author":"Lammie","year":"1998"},{"key":"R16","series-title":"Am J Trop Med Hyg","first-page":"229","article-title":"Multiplex bead assay for serum samples from children in Haiti enrolled in a drug study for the treatment of lymphatic filariasis","volume":"85","author":"Moss","year":"2011"},{"key":"R17","series-title":"PLoS Negl Trop Dis","first-page":"e1941","article-title":"Longitudinal monitoring of the development of antifilarial antibodies and acquisition of Wuchereria bancrofti in a highly endemic area of Haiti","volume":"6","author":"Hamlin","year":"2012"},{"key":"R18","series-title":"J Clin Microbiol","first-page":"882","article-title":"Comparison of formalin-ethyl ether sedimentation, formalin-ethyl acetate sedimentation, and zinic sulfate flotation techniques for detection of intestinal parasites","volume":"13","author":"Truant","year":"1981"},{"key":"R19","series-title":"Infect Immun","first-page":"904","article-title":"Entamoeba histolytica infection in children and protection from subsequent amebiasis","volume":"74","author":"Haque","year":"2006"},{"key":"R20","series-title":"Vaccine","first-page":"611","article-title":"Prevention of intestinal amebiasis by vaccination with the Entamoeba histolytica Gal\/GalNac lectin","volume":"22","author":"Houpt","year":"2004"},{"key":"R21","series-title":"Am J Trop Med Hyg","first-page":"180","article-title":"Haiti: absence of dengue hemorrhagic fever despite hyperendemic dengue virus transmission","volume":"65","author":"Halstead","year":"2001"},{"key":"R22","series-title":"Biometrika","first-page":"13","article-title":"Longitudinal data analysis using generalized linear models","volume":"73","author":"Liang","year":"1986"},{"key":"R23","series-title":"Biometrics","first-page":"1049","article-title":"Models for longitudinal data: a generalized estimating equation approach","volume":"44","author":"Zeger","year":"1998"},{"key":"R24","series-title":"MMWR Surveill Summ","first-page":"1","article-title":"Cryptosporidiosis surveillance\u2013United States 1999\u20132002","volume":"54","author":"Hlavsa","year":"2005"},{"key":"R25","series-title":"MMWR Surveill Summ","first-page":"9","article-title":"Giardiasis surveillance\u2013United States, 1998\u20132002","volume":"54","author":"Hlavsa","year":"2005"},{"key":"R26","series-title":"MMWR","first-page":"1","article-title":"Summary of notifiable diseases\u2013United States, 2010","volume":"59","author":"Centers for Disease 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Wales","volume":"7","author":"Nichols","year":"2009"},{"key":"R36","series-title":"Curr Protoc Cytom","article-title":"Multiplexed microsphere-based flow cytometric immunoassays","author":"Kellar","year":"2006"},{"key":"R37","series-title":"Cytometry","first-page":"27","article-title":"Multiplexed fluorescent bead-based immunoassays for quantitation of human cytokines in serum and culture supernatants","volume":"45","author":"Kellar","year":"2001"},{"key":"R38","series-title":"Korean J Lab Med","first-page":"473","article-title":"Comparative evaluation of ELISA and Luminex panel reactive antibody assays for HLA alloantibody screening","volume":"29","author":"Jung","year":"2009"},{"key":"R39","series-title":"Vet Immunol Immunopathol","first-page":"242","article-title":"Development of a bead-based multiplex assay for simultaneous quantification of cytokines in horses","volume":"127","author":"Wagner","year":"2009"},{"key":"R40","series-title":"Clin Vaccine 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