{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T19:13:25Z","timestamp":1768590805928,"version":"3.49.0"},"reference-count":24,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,6,4]]},"abstract":"<jats:p><jats:italic>Dirofilaria immitis<\/jats:italic> (heartworm) is the causative agent of an important zoonotic disease that is spread by mosquitoes. In this study, molecular and phylogenetic characterization of <jats:italic>D. immitis<\/jats:italic> were performed based on complete ND1 and 16S rDNA gene sequences, which provided the foundation for more advanced molecular diagnosis, prevention, and control of heartworm diseases. The mutation rate and evolutionary divergence in adult heartworm samples from seven dogs in western China were analyzed to obtain information on genetic diversity and variability. Phylogenetic relationships were inferred using both maximum parsimony (MP) and Bayes methods based on the complete gene sequences. The results suggest that <jats:italic>D. immitis<\/jats:italic> formed an independent monophyletic group in which the 16S rDNA gene has mutated more rapidly than has ND1.<\/jats:p>","DOI":"10.4269\/ajtmh.13-0579","type":"journal-article","created":{"date-parts":[[2014,3,18]],"date-time":"2014-03-18T06:17:58Z","timestamp":1395123478000},"page":"1176-1179","source":"Crossref","is-referenced-by-count":5,"title":["Intraspecific Genetic Variation and Phylogenetic Analysis of Dirofilaria immitis Samples from Western China Using Complete ND1 and 16S rDNA Gene Sequences"],"prefix":"10.4269","volume":"90","author":[{"given":"Tianyu","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinan","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuqin","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dandan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaobin","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuerong","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangyou","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Trends Parasitol","first-page":"168","article-title":"Public health issues concerning the widespread distribution of canine heartworm disease","volume":"26","author":"Lee","year":"2010"},{"key":"R2","series-title":"Textbook of Small Animal Veterinary Medicine","author":"Ettinger","year":"2005"},{"key":"R3","series-title":"Canine heartworm disease. 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