{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:28:49Z","timestamp":1790717329094,"version":"4.1.0"},"reference-count":62,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,9,3]]},"abstract":"<jats:p>\n                    During 2010 and 2011, the Loreto region of Peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. This outbreak coincided with the reappearance of dengue virus-2 (DENV-2) in Loreto after almost 8 years. Whole-genome sequence indicated that DENV-2 from the outbreak belonged to lineage II of the southeast Asian\/American genotype and was most closely related to viruses circulating in Brazil during 2007 and 2008, whereas DENV-2 previously circulating in Loreto grouped with lineage I (DENV-2 strains circulating in South America since 1990). One amino acid substitution (NS5 A811V) in the 2010 and 2011 isolates resulted from positive selection. However, the 2010 and 2011 DENV-2 did not replicate to higher titers in monocyte-derived dendritic cells and did not infect or disseminate in a higher proportion of\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    than DENV-2 isolates previously circulating in Loreto. These results suggest that factors other than enhanced viral replication played a role in the severity of this outbreak.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.13-0600","type":"journal-article","created":{"date-parts":[[2014,7,8]],"date-time":"2014-07-08T01:00:12Z","timestamp":1404781212000},"page":"611-620","source":"Crossref","is-referenced-by-count":61,"title":["Lineage II of Southeast Asian\/American DENV-2 is Associated with a Severe Dengue Outbreak in the Peruvian Amazon"],"prefix":"10.4269","volume":"91","author":[{"given":"Maya","family":"Williams","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra V.","family":"Mayer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William 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