{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T19:59:18Z","timestamp":1762459158544},"reference-count":49,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,7,2]]},"abstract":"<jats:p>The immune response to dengue virus (DENV) infection generates high levels of antibodies (Abs) against the DENV non-structural protein 1 (NS1), particularly in cases of secondary infection. Therefore, anti-NS1 Abs may play a role in severe dengue infections, possibly by interacting (directly or indirectly) with host factors or regulating virus production. If it does play a role, NS1 may contain epitopes that mimic those epitopes of host molecules. Previous attempts to map immunogenic regions within DENV-NS1 were undertaken using mouse monoclonal Abs (MAbs). The aim of this study was to characterize the epitope regions of nine anti-NS1 human monoclonal Abs (HuMAbs) derived from six patients secondarily infected with DENV-2. These anti-NS1 HuMAbs were cross-reactive with DENV-1, -2, and -3 but not DENV-4. All HuMAbs bound a common epitope region located between amino acids 221 and 266 of NS1. This study is the first report to map a DENV-NS1 epitope region using anti-DENV MAbs derived from patients.<\/jats:p>","DOI":"10.4269\/ajtmh.13-0624","type":"journal-article","created":{"date-parts":[[2014,4,29]],"date-time":"2014-04-29T07:38:13Z","timestamp":1398757093000},"page":"146-155","source":"Crossref","is-referenced-by-count":17,"title":["A Highly Conserved Region Between Amino Acids 221 and 266 of Dengue Virus Non-Structural Protein 1 is a Major Epitope Region in Infected Patients"],"prefix":"10.4269","volume":"91","author":[{"given":"Magot Diata","family":"Omokoko","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabar","family":"Pambudi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Supranee","family":"Phanthanawiboon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Promsin","family":"Masrinoul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chayanee","family":"Setthapramote","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadahiro","family":"Sasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Motoki","family":"Kuhara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pongrama","family":"Ramasoota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akifumi","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itaru","family":"Hirai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyoshi","family":"Ikuta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Kurosu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Arch Med Res","first-page":"330","article-title":"The global emergence\/resurgence of arboviral diseases as public health problems","volume":"33","author":"Gubler","year":"2002"},{"key":"R2","series-title":"J Gen Virol","first-page":"37","article-title":"Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera","volume":"70","author":"Calisher","year":"1989"},{"key":"R3","series-title":"Annu Rev Microbiol","first-page":"649","article-title":"Flavivirus: genome organization, expression, and replication","volume":"44","author":"Chambers","year":"1990"},{"key":"R4","series-title":"Yale J Biol Med","first-page":"311","article-title":"Observations related to pathogenesis of dengue hemorrhagic fever. IV. Relation of disease severity to antibody response and virus recovered","volume":"42","author":"Halstead","year":"1970"},{"key":"R5","series-title":"Bull World Health Organ","first-page":"375","article-title":"Dengue haemorrhagic fever\/dengue shock syndrome: lessons from the Cuban epidemic","volume":"67","author":"Kouri","year":"1981"},{"key":"R6","series-title":"J Virol","first-page":"6104","article-title":"Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion","volume":"73","author":"Flamand","year":"1999"},{"key":"R7","series-title":"J Virol Methods","first-page":"67","article-title":"Characterization of dengue virus NS1 stably expressed in 293T cell lines","volume":"142","author":"Noisakran","year":"2007"},{"key":"R8","series-title":"J Gen Virol","first-page":"1183","article-title":"Glycosylation mutants of dengue virus NS1 protein","volume":"75","author":"Pryor","year":"1994"},{"key":"R9","series-title":"Proc Natl Acad Sci USA","first-page":"8003","article-title":"Secreted dengue virus nonstructural protein NS1 is an atypical barrel-shaped high-density lipoprotein","volume":"108","author":"Gutsche","year":"2011"},{"key":"R10","series-title":"J Gen Virol","first-page":"2107","article-title":"The antigenic structure of dengue type 1 virus envelope and NS1 proteins expressed in Escherichia coli","volume":"71","author":"Mason","year":"1990"},{"key":"R11","series-title":"J Biol Chem","first-page":"20729","article-title":"Determination of the disulfide bond arrangement of dengue virus NS1 protein","volume":"279","author":"Wallis","year":"2004"},{"key":"R12","series-title":"Virology","first-page":"769","article-title":"The effects of site-directed mutagenesis on the dimerization and secretion of the NS1 protein specified by dengue virus","volume":"194","author":"Pryor","year":"1993"},{"key":"R13","series-title":"J Immunol","first-page":"838","article-title":"Immunoprecipitation analysis of soluble complement-fixing antigens of dengue viruses","volume":"105","author":"Russell","year":"1970"},{"key":"R14","series-title":"J Infect Dis","first-page":"1078","article-title":"Vascular leakage in severe dengue virus infections: a potential role for the nonstructural viral protein NS1 and complement","volume":"193","author":"Avirutnan","year":"2006"},{"key":"R15","series-title":"Curr Opin Infect Dis","first-page":"429","article-title":"Immunopathological mechanisms in dengue and dengue hemorrhagic fever","volume":"19","author":"Green","year":"2006"},{"key":"R16","series-title":"J Clin Microbiol","first-page":"376","article-title":"Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 Reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections","volume":"40","author":"Alcon","year":"2002"},{"key":"R17","series-title":"Virol J","first-page":"47","article-title":"Kinetics of non-structural protein 1, IgM and IgG antibodies in dengue type 1 primary infection","volume":"8","author":"Hu","year":"2011"},{"key":"R18","series-title":"J Infect Dis","first-page":"1165","article-title":"High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever","volume":"186","author":"Libraty","year":"2002"},{"key":"R19","series-title":"J Clin Microbiol","first-page":"1053","article-title":"An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients","volume":"38","author":"Young","year":"2000"},{"key":"R20","series-title":"Clin Diagn Lab Immunol","first-page":"856","article-title":"Human dengue antibodies against structural and nonstructural proteins","volume":"7","author":"Vald\u00e9s","year":"2000"},{"key":"R21","series-title":"J Exp Med","first-page":"793","article-title":"Antagonism of the complement component C4 by flavivirus nonstructural protein NS1","volume":"207","author":"Avirutnan","year":"2010"},{"key":"R22","series-title":"J Immunol","first-page":"424","article-title":"Binding of flavivirus nonstructural protein NS1 to C4b binding protein modulates complement activation","volume":"187","author":"Avirutnan","year":"2011"},{"key":"R23","series-title":"Mol Immunol","first-page":"398","article-title":"Anti-dengue virus nonstructural protein 1 antibodies recognize protein disulfide isomerase on platelets and inhibit platelet aggregation","volume":"47","author":"Cheng","year":"2009"},{"key":"R24","series-title":"J Med Virol","first-page":"82","article-title":"Antibodies from dengue patient sera cross-react with endothelial cells and induce damage","volume":"69","author":"Lin","year":"2003"},{"key":"R25","series-title":"J Biol Chem","first-page":"9726","article-title":"Molecular mimicry of human endothelial cell antigen by autoantibodies to nonstructural protein 1 of dengue virus","volume":"286","author":"Liu","year":"2011"},{"key":"R26","series-title":"Biochem Biophys Res Commun","first-page":"867","article-title":"Human monoclonal antibodies to neutralize all dengue virus serotypes using lymphocytes from patients at acute phase of the secondary infection","volume":"423","author":"Setthapramote","year":"2012"},{"key":"R27","series-title":"Biochem Biophys Res Commun","first-page":"1051","article-title":"Secreted complement regulatory protein clusterin interacts with dengue virus nonstructural protein 1","volume":"362","author":"Kurosu","year":"2007"},{"key":"R28","series-title":"Immunol Lett","first-page":"57","article-title":"Interaction sites on human IgG-Fc for FcgammaR: current models","volume":"82","author":"Jefferis","year":"2002"},{"key":"R29","series-title":"Virology","first-page":"290","article-title":"Comprehensive mapping of immunodominant and conserved serotype- and group-specific B-cell epitopes of nonstructural protein 1 from dengue virus type 1","volume":"398","author":"Chen","year":"2010"},{"key":"R30","series-title":"Clin Vaccine Immunol","first-page":"549","article-title":"Monoclonal antibodies that bind to common epitopes on the dengue virus type 2 nonstructural-1 and envelope glycoproteins display weak neutralizing activity and differentiated responses to virulent strains: implications for pathogenesis and vaccines","volume":"15","author":"Falconar","year":"2008"},{"key":"R31","series-title":"Arch Virol","first-page":"315","article-title":"Precise location of sequential dengue virus subcomplex and complex B cell epitopes on the nonstructural-1 glycoprotein","volume":"137","author":"Falconar","year":"1994"},{"key":"R32","series-title":"Virus Res","first-page":"49","article-title":"Selection and identification of B-cell epitope on NS1 protein of dengue virus type 2","volume":"150","author":"Jiang","year":"2010"},{"key":"R33","series-title":"Jpn J Infect Dis","first-page":"109","article-title":"Highly conserved region 141\u2013168 of the NS1 protein is a new common epitope region of dengue virus","volume":"64","author":"Masrinoul","year":"2011"},{"key":"R34","series-title":"Virology","first-page":"93","article-title":"Functional and antigenic domains of the dengue-2 virus nonstructural glycoprotein NS-1","volume":"163","author":"Putnak","year":"1988"},{"key":"R35","series-title":"J Med Virol","first-page":"490","article-title":"Nonstructural protein NS1 immunodominant epitope detected specifically in dengue virus infected material by a SELDI-TOF\/MS based assay","volume":"84","author":"Steidel","year":"2012"},{"key":"R36","series-title":"J Clin Microbiol","first-page":"977","article-title":"Identification of B-cell epitope of dengue virus type 1 and its application in diagnosis of patients","volume":"39","author":"Wu","year":"2001"},{"key":"R37","series-title":"FEMS Microbiol Lett","first-page":"93","article-title":"A serotype-specific epitope of dengue virus 1 identified by phage displayed random peptide library","volume":"127","author":"Yao","year":"1995"},{"key":"R38","series-title":"J Infect Dis","first-page":"743","article-title":"Facilitation of cell adhesion by immobilized dengue viral nonstructural protein 1 (NS1): arginine-glycine-aspartic acid structural mimicry within the dengue viral NS1 antigen","volume":"186","author":"Chang","year":"2002"},{"key":"R39","series-title":"Arch Virol","first-page":"897","article-title":"The dengue virus nonstructural-1 protein (NS1) generates antibodies to common epitopes on human blood clotting, integrin\/adhesin proteins and binds to human endothelial cells: potential implications in haemorrhagic fever pathogenesis","volume":"142","author":"Falconar","year":"1997"},{"key":"R40","series-title":"Free Radic Biol Med","first-page":"85","article-title":"Antibodies against dengue virus nonstructural protein-1 induce heme oxygenase-1 via a redox-dependent pathway in human endothelial cells","volume":"54","author":"Immenschuh","year":"2013"},{"key":"R41","series-title":"J Immunol","first-page":"657","article-title":"Endothelial cell apoptosis induced by antibodies against dengue virus nonstructural protein 1 via production of nitric oxide","volume":"169","author":"Lin","year":"2002"},{"issue":"(Suppl 5)","key":"R42","series-title":"BMC Bioinformatics","first-page":"S4","article-title":"Large-scale analysis of antigenic diversity of T-cell epitopes in dengue virus","volume":"7","author":"Khan","year":"2006"},{"key":"R43","series-title":"J Virol","first-page":"1340","article-title":"Antibodies against West Nile Virus nonstructural protein NS1 prevent lethal infection through Fc \u03b3 receptor-dependent and -independent mechanisms","volume":"80","author":"Chung","year":"2006"},{"key":"R44","series-title":"Antiviral Res","first-page":"192","article-title":"The flavivirus NS1 protein: molecular and structural biology, immunology, role in pathogenesis and application as a diagnostic biomarker","volume":"98","author":"Muller","year":"2013"},{"key":"R45","series-title":"Science","first-page":"881","article-title":"Flavivirus NS1 structures reveal surfaces for associations with membranes and the immune system","volume":"343","author":"Akey","year":"2014"},{"key":"R46","series-title":"Ric Clin Lab","first-page":"463","article-title":"Human IgG subclasses in health and disease. 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