{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:19:51Z","timestamp":1790745591150,"version":"4.1.0"},"reference-count":12,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,6,4]]},"abstract":"<jats:p>Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (<jats:italic>Burkholderia pseudomallei<\/jats:italic> and <jats:italic>Burkholderia mallei<\/jats:italic>, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify <jats:italic>B. pseudomallei<\/jats:italic> and <jats:italic>B. mallei<\/jats:italic> isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by <jats:italic>B. pseudomallei<\/jats:italic> and <jats:italic>B. mallei<\/jats:italic>, but is absent in closely related <jats:italic>Burkholderia<\/jats:italic> species. A total of 110 <jats:italic>B. pseudomallei<\/jats:italic> and <jats:italic>B. mallei<\/jats:italic> were tested, and 36 closely related <jats:italic>Burkholderia<\/jats:italic> species. The latex agglutination assay was positive for 109 of 110 (99.1% sensitivity) <jats:italic>B. pseudomallei<\/jats:italic> and <jats:italic>B. mallei<\/jats:italic> isolates tested.<\/jats:p>","DOI":"10.4269\/ajtmh.14-0025","type":"journal-article","created":{"date-parts":[[2014,4,8]],"date-time":"2014-04-08T06:06:30Z","timestamp":1396937190000},"page":"1043-1046","source":"Crossref","is-referenced-by-count":63,"title":["Evaluation of a Latex Agglutination Assay for the Identification of Burkholderia pseudomallei and Burkholderia mallei"],"prefix":"10.4269","volume":"90","author":[{"given":"Brea D.","family":"Duval","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mindy G.","family":"Elrod","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay E.","family":"Gee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narisara","family":"Chantratita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarunporn","family":"Tandhavanant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Direk","family":"Limmathurotsakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex R.","family":"Hoffmaster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"J Clin Microbiol","first-page":"3774","article-title":"Accuracy of Burkholderia pseudomallei identification using the API 20NE system and a latex agglutination test","volume":"45","author":"Amornchai","year":"2007"},{"key":"R2","series-title":"J Med Microbiol","first-page":"1075","article-title":"Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia","volume":"49","author":"Anuntagool","year":"2000"},{"key":"R3","series-title":"Am J Trop Med Hyg","first-page":"735","article-title":"Short report: evaluation of a monoclonal antibody-based latex agglutination test for rapid diagnosis of septicemic melioidosis","volume":"61","author":"Samosornsuk","year":"1999"},{"key":"R4","series-title":"Am J Trop Med Hyg","first-page":"759","article-title":"Short report: a rapid method for the differentiation of Burkholderia pseudomallei and Burkholderia thailandensis","volume":"66","author":"Wuthiekanun","year":"2002"},{"key":"R5","series-title":"J Infect Dis","first-page":"103","article-title":"Wound infection by an indigenous Pseudomonas pseudomallei-like organism isolated from the soil: case report and epidemiologic study","volume":"135","author":"McCormick","year":"1977"},{"key":"R6","series-title":"Arch Ophthalmol","first-page":"1224","article-title":"Pseudomonas pseudomallei in an anopthalmic orbit","volume":"98","author":"Nussbaum","year":"1980"},{"key":"R7","series-title":"Biomed Environ Sci","first-page":"101","article-title":"Studies on fermented corn flour poisoning in rural areas of China. I. Epidemiology, clinical manifestations, and pathology","volume":"1","author":"Meng","year":"1988"},{"key":"R8","series-title":"Eur J Pediatr","first-page":"1503","article-title":"Burkholderia gladioli sepsis in newborns","volume":"171","author":"Dursun","year":"2012"},{"key":"R9","series-title":"Clin Infect Dis","first-page":"1291","article-title":"Severe Burkholderia (Pseudomonas) gladioli infection in chronic granulomatous disease: report of two successfully treated cases","volume":"21","author":"Ross","year":"1995"},{"key":"R10","series-title":"MBio","first-page":"e00388","article-title":"Within-host evolution of Burkholderia pseudomallei over a twelve-year chronic carriage infection","volume":"4","author":"Price","year":"2013"},{"key":"R11","series-title":"Infect Immun","first-page":"1209","article-title":"Characterization of the Burkholderia pseudomallei K96243 capsular polysaccharide I coding region","volume":"80","author":"Cuccui","year":"2012"},{"key":"R12","series-title":"Genome Biol","first-page":"R89","article-title":"Genomic acquisition of a capsular polysaccharide virulence cluster by non-pathogenic Burkholderia isolates","volume":"11","author":"Sim","year":"2010"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/90\/6\/article-p1043.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/90\/6\/article-p1043.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/90\/6\/article-p1043.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T21:25:28Z","timestamp":1723065928000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/90\/6\/article-p1043.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,4]]},"references-count":12,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.14-0025","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,4]]}}}